Tibetan mastiff sperm freezing diluent

A technology of freezing diluents and diluents, which is applied in the preservation of microorganisms, human or animal bodies, biochemical equipment and methods, etc., and can solve problems such as the difference in conception rate and average number of litters, so as to reduce damage and improve freezing efficiency. The effect of the protective effect

Inactive Publication Date: 2009-01-28
张尤嘉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the current research level and natural reproduction, there is still a certain gap in the conception rate and the average number of litters.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1. A frozen diluent of Tibetan mastiff semen, the diluent is composed of liquid 1 and liquid 2, the components and weight of liquid 1 are: calcium chloride 0.30g, potassium chloride 0.25g, magnesium chloride 0.32g, sodium chloride 5.85g , sodium dihydrogen phosphate 0.45g, sodium bicarbonate 2.38g, sodium pyruvate 0.025g, add double distilled water to dilute to a certain volume, and heat to dissolve, filter and sterilize with the conventional method in this field, leave the filtrate as 1 solution; 2 The liquid is based on the 1 liquid, under aseptic conditions, take 1 liquid 50mL, mix with calf serum 25mL, egg yolk 20mL, glycerin 5mL, penicillin 1000IU / mL and mix to form the described dilution, and the osmotic pressure of this dilution is 325mosmo / L, with a pH value of 6.8, which has an excellent effect on cryopreservation of Tibetan mastiff semen. When used, dilute it with Tibetan mastiff semen at a ratio of 1:2 at the same temperature.

Embodiment 2

[0015] Example 2. A frozen diluent of Tibetan mastiff semen, the diluent is composed of liquid 1 and liquid 2, the components and weight of liquid 1 are: calcium chloride 0.20g, potassium chloride 0.18g, magnesium chloride 0.22g, sodium chloride 5.0g , sodium dihydrogen phosphate 0.3g, sodium bicarbonate 1.38g, sodium pyruvate 0.03g, add double distilled water to make up to 1L, and heat to dissolve, filter and sterilize with conventional methods in this field, and keep the filtrate as 1 liquid; 2 The solution is based on the 1 solution, under aseptic conditions, take 1 solution 35mL, mix with calf serum 30mL, egg yolk 25mL, glycerin 10mL, penicillin 1000IU / mL and mix to form the described dilution, and the osmotic pressure of the dilution is 310mosmo / L, pH value is 6.5. The prepared diluent has an excellent effect on cryopreservation of Tibetan mastiff semen. When used, dilute it with Tibetan mastiff semen at a ratio of 1:2 at the same temperature.

Embodiment 3

[0016] Example 3. A frozen diluent of Tibetan mastiff semen, the diluent is composed of liquid 1 and liquid 2, the components and weight of liquid 1 are: calcium chloride 0.50g, potassium chloride 0.36g, magnesium chloride 0.45g, sodium chloride 3.5g , sodium dihydrogen phosphate 0.55g, sodium bicarbonate 3.2g, sodium pyruvate 0.036g, add double distilled water to 1L, and heat to dissolve, filter and sterilize with conventional methods in this field, and keep the filtrate as 1 liquid; 2 The solution is based on the 1 solution, under aseptic conditions, take 1 solution 55mL, mix with calf serum 20mL, egg yolk 20mL, glycerin 5mL, penicillin 1000IU / mL and mix to form the described dilution solution, and the osmotic pressure of the dilution solution is 330mosmo / L, pH value is 7, it has an excellent effect on cryopreservation of Tibetan mastiff semen. When used, dilute it with Tibetan mastiff semen at a ratio of 1:2 at the same temperature.

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PUM

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Abstract

The invention relates to a cryogenic diluent of the semen of Tibetan mastiffs, which belongs to the technical field of animal reproduction and comprises 1 fluid and 2 fluid; wherein, the 1 fluid is prepared by the following steps: the raw materials comprise 0.20 to 0.50g of calcium chloride, 0.18 to 0.36g of potassium chloride, 0.22 to 0.45g of magnesium chloride, 3.5 to 5.85g of sodium chloride, 0.30 to 0.55g of monosodium phosphate, 1.38 to 3.20g of sodium bicarbonate and 0.025 to 0.036g of sodium pyruvate, and double distilled water is added to obtain constant volume of 1L; the 2 fluid is prepared by the following steps: 35 to 55ml of the 1 fluid, 20 to 30ml of calf serum, 20 to 25ml of yolk, 5 to 10ml of glycerin and 1000IU / ml of penicillin are mixed to prepare to the diluent with the penetrating pressure of 300 to 330mosmo / l and the pH value of 6.5 to 7.0; the diluent leads the activity of the cryogenic diluent of the semen of Tibetan mastiffs to reach 0.6 to 0.7, and the conception rate and the average number of litter size to respectively reach 77.3 percent to 84 percent and four to five so as to reach or near the selfing level, thus being a cryogenic diluent of the semen of Tibetan mastiff with practical application and promotion value.

Description

technical field [0001] The invention relates to a frozen diluent of Tibetan mastiff semen, which is used for freezing and artificial insemination of Tibetan mastiff semen, and belongs to the technical field of animal reproduction. Background technique [0002] Canine semen freezing has high application value, and the use of canine semen freezing and artificial insemination has many advantages over natural breeding. For example: it can highly utilize the utilization rate of excellent breeding dogs; reduce the spread of various diseases, especially the spread of reproductive system diseases; it is not limited by time and space, it is convenient and quick to introduce, and it is cost-effective and high-effective; germplasm resources can be effectively preserved, In particular, it has important practical significance for the protection and development of Tibetan mastiff species in my country. [0003] At present, there are few studies on semen freezing and artificial inseminatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A01N1/02
Inventor 张尤嘉张振韬
Owner 张尤嘉
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