131results about How to "Maintain osmotic pressure" patented technology

The invention relates to the fields of medicine and biology, in particular to an organ preservation solution and a preparation method thereof. Every 1000mL of the organ preservation solution is prepared from the following components by weight: 3.0-15.0g of sodium citrate, 10.0-30.0g of potassium citrate, 5.0-15.0g of mannitol, 0.5-1.2g of potassium chloride, 0.5-1.8g of magnesium sulfate heptahydrate, 0.5-25.0g of a pH buffer pair, 0.2-25.0g of energy substrate and the balance of water for injection. The organ preservation solution also contains one or more of laminarin, trehalose, laminine and betaine. The organ preservation solution is low in price and efficient, and is suitable for preserving multiple organs.

The invention discloses a sleep-aiding probiotic solid beverage and a preparation method thereof in the field of food processing. The sleep-aiding probiotic solid beverage is prepared from gamma-aminobutyric acid, theanine, blueberry powder, mogroside, erythritol, probiotics and prebiotics. The probiotics comprise bifidobacterium lactis, lactobacillus acidophilus and lactobacillus fermentum; the prebiotics comprise fructo-oligosaccharide, resistant dextrin and lactitol; by adopting a sterile dry-mixing process under low-temperature and low-humidity conditions, the solid beverage is natural inraw materials, scientific in formula and fresh and cool in taste, can be directly eaten, can also be taken after being mixed with warm water, and is suitable for various people, especially people withpoor sleep, anxiety and the like. The gamma-aminobutyric acid, theanine, blueberry powder and various prebiotics and probiotics are combined for use, so that field planting and proliferation of the probiotics after intake can be guaranteed, intestinal health can be protected, the requirement for sleep improvement can be met, anxiety can be relieved, safety and mildness are achieved, and dependence is avoided.

The invention discloses a chicken feed, and belongs to the technical field of poultry feeds. The chicken feed is prepared from the following raw materials in parts by weight: 65 parts of corn, 24 parts of soybean meal, 3 parts of fish meal, 0.7 part of dicalcium phosphate, 0.3 part of phytate phosphorus, 0.715 part of shell powder, 0.185 part of an additive, 0.4 part of sodium sulfate, 0.3 part of table salt, 0.4 part of astragalus membranaceus multi vitamin, and 5 parts of a green feed, wherein the green feed is one or more of pasturage, pine needle powder and herba euphorbiae thymifoliae; the additive is the mixture of stone powder, choline chloride, methionine and lysine. The feed can meet various nutritional requirements for the growth and the development of chickens.

The invention discloses an improved formulation of blood agar culture medium, made from tryptone, beef extract powder, liver digest, yeast extract, starch, sodium chloride, defibered sheep blood and agar, wherein each 1000 ml of the culture medium contains 10-20 g of tryptone, 3-8 g of beef extract powder, 1-4 g of liver digest, 0.5-2 g of starch, 3-8 g of yeast powder, 3-8 parts of sodium chloride, 40-60 ml of defibered sheep blood, 10-20 g of agar, and has pH of 7.2+/-0.2. The improved formulation can provide complete nutrition, maintain osmotic pressure for bacteria, and repair the damaged bacteria. The formulation optimized herein may meet the complete demands of common bacteria and most fastidious bacteria, and is good for the cultivation and identification of bacteria.

The invention relates to a combined medicament for supplementing zinc and calcium and a preparation method thereof. Medicament components are released at a fixed part. The preparation method comprises the following steps of: taking zinc gluconate and vitamin D, adding medicinal starch and microcrystalline cellulose and mixing, adding crushed superfine silica gel powder and evenly mixing and sub-packaging into enteric capsules; and mixing water-soluble calcium acetate, the medicinal starch and the microcrystalline cellulose, adding the crushed superfine silica gel powder and evenly mixing and sub-packaging into Muslim capsules. The medicament and the preparation method thereof ensure that the vitamin D cannot be damaged in an acid environment, avoid the direct stimulation of the zinc gluconate to the stomach and block off the direct contact of vitamin E and oxygen. After patients take two kinds of capsules, effective components of the vitamin E and the calcium acetate are rapidly disintegrated and absorbed in the stomach; the vitamin D and the zinc gluconate are disintegrated and absorbed in the intestine; and the invention has the characteristics of taking effect of medicament, high bioavailability, small side effect and the like, and is specially suitable for children, pregnant women, adults and old people for supplement and administration for a long term.

The invention relates to a large-scale production method for clostridium butyricum. The method comprises the following steps: (1) preparing a clostridium butyricum suspension with clostridium butyricum colonies of slant culture; (2) inoculating the clostridium butyricum suspension into fermentation broth in a seed tank, and performing anaerobic culture to a logarithmic growth phase to obtain fermentation strains; and (3) inoculating fermentation strains into the fermentation broth in a fermentation tank, and performing large-scale anaerobic fermentation production to obtain a clostridium butyricum product with a living bacteria number greater than 10<8>CFU/ml. The method prepares the clostridium butyricum suspension with a clostridium butyricum lawn on a slant culture medium, and then inoculates the clostridium butyricum suspension into the seed tank for anaerobic fermentation, and experimental results show that the same fermentation effect as a clostridium butyricum fermentation seedsolution can be achieved; the method does not require secondary expanding culture, so that the fermentation cycle is shortened by at least one primary seed fermentation cycle in the entire process flow; and meanwhile, at least one strain passage is also reduced, thus reducing the risk of strain variation caused by excessive strain passage.

The invention discloses a separating mediumon solution for removing dead hepatocytes and a method for removing dead hepatocytes by utilizing the separating mediumon solution and belongs to the technical field of hepatocyte isolationliver cell separation and purification. The separating mediumon solution is prepared by adding the following components in a William's E culture medium: 0.1-1.5 percentv/v of ITS universal culture additives, 1.5-2.5 mmol/L of L-glutamine, 1-20 [mu]gmu g/L of an epidermal growth factor, 15-20mg/L of hydrocortisone, 35-45 [mu]gmu g/L of dexamethasone, 0.5-1 percent v/v of penicillin streptomycin, 1-10 percent v/v fetal calf serum and 0.25-0.5g/mL of Nycodenz. The separating medium tion solution has the advantages of maintaining normal osmotic pressure of hepatocytes, repairing partial damaged hepatocytes and providing nutritional requirement for hepatocytes.

The invention relates to a treating fluid used for the cultivation of nucleus pearls, and belongs to the technical field of pearl cultivation. The treating fluid comprises the following components: (a),a first treating fluid and (b), a second treating fluid, wherein the first treating fluid comprises the components as follows: a bamboo charcoal powder solution, a dry-chrysanthemum solution, a gold mudstone solution, a gobi stone solution, a safflower parasitic skin solution, a peach parasitic skin solution, a safflower loranthus parasiticus skin solution and a vitamin B solution; the second treating fluid comprises the components as follows: acid red, chondroitin, mafenide, taurine, sodium chloride, hyriopsis cumingii polysacchride, vitronectin and water. According to the invention, through using the treating fluid for cultivation, the technical problems that the peals cultivated by the traditional method are dim in luster, poor in quality and low in survival rate are solved.

The invention relates to the field of sperm freezing, and particularly relates to a sperm freezing medium as well as a preparation method and application thereof. The sperm freezing medium comprises the following components: potassium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, dipotassium phosphate, sodium pyruvate, calcium lactate, glucose, saccharose, glutamine, glycine,HEPES, gentamicin, glycerinum, human serum albumin, vitamin E, vitamin C and vitamin B12. According to the sperm freezing medium, all components are matched with each other to comprehensively maintainthe osmotic pressure, the straining capacity of sperm to resist the outside environment change; the freezing medium is used for storing sperm in low temperature; the sperm survive rate and the activity can be maintained for a long term.

The invention belongs to the field of waste water biological strengthening treatment, and concretely relates to an activation promoter capable of increasing microbiological treatment effect of daily chemical waste water. The daily chemical waste water has the characteristics of high organic matter, high salt and biological toxicity, and treatment by using a conventional biology method is difficultto reach the standard. The activation promoter comprises chitosan, glycine betaine, ferrous sulphate, trehalose, citric acid, proline, and composite vitamin, can effectively improve the survival breeding capability and metabolic activity of microbe under conditions of high organic matter and high salt, and can effectively increase the sewage processing effect. The activation promoter has the advantages of low cost and convenient making, and can be used for biological treatment of daily chemical waste water and other high-salt surfactant waste water.

The invention discloses a novel preservation solution for an alveolar lavage fluid microbial nucleic acid. The preservation solution comprises a treatment solution I and a treatment solution II, wherein the treatment solution I comprises a guanidine hydrochloride buffer solution, a nuclease inhibitor, a metabolism inhibitor, an alkali metal halide and glycerol, and the treatment solution II comprises an alkali metal hydroxide and a surfactant. The treatment solution I and the treatment solution II which are separately stored are used as the novel preservation solution for the alveolar lavage fluid microbial nucleic acid, so that the preservation solution can be prevented from generating precipitates, at the same time, different interference impurities can be treated respectively in a targeted manner, alveolar lavage fluid samples can be rapidly stabilized, the activity of nuclease is effectively inhibited, the cell lysis is prevented, at the same time, the free nucleic acid in the alveolar lavage fluid can be well protected, the preservation time is long, the accuracy and convenience of nucleic acid detection are improved, and thereby the preservation solution has great applicationvalue in the detection and diagnosis of tumor diseases, viruses and the like.

The invention discloses an industrial fermentation method of phytase. The method comprises the following steps: (1) fermentation in a first-level fermentation tank; (2) fermentation in a second-levelfermentation tank; (3) high-density fermentation; (4) fed-batch fermentation and starving; (5) induction and feed mixing. The industrial fermentation method of phytase disclosed by the invention has the characteristics of high tank enzyme activity and high fermentation efficiency.

The invention relates to the field of fresh keeping of seafoods, in particular to a fresh keeping method of seafoods. The fresh keeping method of the shellfish seafoods comprises the steps of placingfresh seafoods to a heat-insulation container loaded with an edible salt solution of minus 2 to 2 DEG C, and placing the heat-insulation container to be environment of minus 3 to 0 DEG C. According tothe fresh keeping method of the seafood, the fresh keeping time of the seafoods can be prolonged to 15 days or above, the economic value of the seafoods increased, and the fresh keeping method has high promotion value.