131results about How to "Maintain osmotic pressure" patented technology

The composite mycose nutriment with the health functions of repairing gastrointestinal mucous membrane, resisting tumor, protecting liver and regulating immunity consists of lentinan powder, ash tree flower polysaccharide powder, morel polysaccharide powder, wolfberry fruit polysaccharide, whey protein powder, vinegar, honey, haw portions, barley malt powder, composite vitamins, zinc lactate, calcium carbonate, oligoxylose and water. It has the nutrients and effective components of its ingredients well maintained and has the health functions of resisting chemical mutagensis, resisting virus, regulating blood fat, regulating immunity, resisting platelet aggregation, resisting tumor, etc.

A nutritive protein powder containing probiotics for improving immunity, preventing and treating constipation and diarrhea, and delaying samility contains proportionally whey protein powder, oligoxylose powder, guar gum, acidophilic latobacillus, bifidobacterium, beta-cyclodextrin, white sugar, milk powder essence, VB1, VB2, zinc lactate, and ferrous sulfate.

The invention discloses an inulin soya-bean milk beverage and a preparation method thereof, belonging to the technical field of food processing. The inulin soya-bean milk beverage disclosed by the invention mainly consists of the following components in parts by weight: 80-120 parts of soya-bean milk, 2-20 parts of inulin, 1-5 parts of honey and 0.1-0.4 parts of a stabilizer, wherein the inulin, which is also called as synanthrin, is linear straight-chain polysaccharide formed by D-fructose through key jointing of beta (1 to 2), the tail end of the inulin is provided with a glucose residue, and the degree of polymerization is 2-60. The inulin, as a soluble dietary fiber different from a starch structure, can selectively promote the growth of colon probiotics and improve the health condition of a host. The inulin soya-bean milk beverage disclosed by the invention is white, is exquisite and smooth in mouth feel, has a low caloric value and can promote the growth of human colon probiotics, inhibit toxic fermentation products, promote the absorption of mineral materials such as calcium, magnesium and iron and improve the physiological functions such as immunity of the organism.

The invention relates to the fields of medicine and biology, in particular to an organ preservation solution and a preparation method thereof. Every 1000mL of the organ preservation solution is prepared from the following components by weight: 3.0-15.0g of sodium citrate, 10.0-30.0g of potassium citrate, 5.0-15.0g of mannitol, 0.5-1.2g of potassium chloride, 0.5-1.8g of magnesium sulfate heptahydrate, 0.5-25.0g of a pH buffer pair, 0.2-25.0g of energy substrate and the balance of water for injection. The organ preservation solution also contains one or more of laminarin, trehalose, laminine and betaine. The organ preservation solution is low in price and efficient, and is suitable for preserving multiple organs.

The present invention relates to conjugates of hemoglobin and human serum albumin and the preparation process thereof. The conjugates have molecular weight in the range of 100-300 kD, comprising 1-3 intermolecularly or intramolecularly cross-linked hemoglobin molecules and 1-3 human serum albumin molecules. The conjugates are obtained by the following steps: preparing stroma-free hemoglobin, then coupling hemoglobin to human serum albumin and purifying the products using anion exchange chromatography. The hemoglobin and human serum albumin conjugates have characteristics suitable for being used as blood substitutes.

The invention discloses rhodomyrtus tomentosa liqueur and a preparation method thereof. According to the invention, vegetable fat is extracted through enzymes, oil and non-oil ingredients are separated by using an affinity difference of non-oil ingredients to oil and water and different specific gravity of oil and water, and the taste, color and luster and guarantee period of the final product are ensured.

The invention relates to an exosome preserving fluid and an exosome preserving method, which belong to the technical field of biology. The exosome preserving fluid comprises bovine serum albumin, trehalose, glycerol, dimethyl sulfoxide and a phosphate buffer solution. The invention also provides the exosome preserving method comprises the following steps: heating the preserving fluid at 85-95 DEGC for 10-30 minutes to form a colloidal solution, and preserving the exosome in a colloidal solution. The exosome preserving fluid provided by the invention can maintain the integrity, biological activity and inclusion stability of exosomes for a long time.

The invention discloses a sleep-aiding probiotic solid beverage and a preparation method thereof in the field of food processing. The sleep-aiding probiotic solid beverage is prepared from gamma-aminobutyric acid, theanine, blueberry powder, mogroside, erythritol, probiotics and prebiotics. The probiotics comprise bifidobacterium lactis, lactobacillus acidophilus and lactobacillus fermentum; the prebiotics comprise fructo-oligosaccharide, resistant dextrin and lactitol; by adopting a sterile dry-mixing process under low-temperature and low-humidity conditions, the solid beverage is natural inraw materials, scientific in formula and fresh and cool in taste, can be directly eaten, can also be taken after being mixed with warm water, and is suitable for various people, especially people withpoor sleep, anxiety and the like. The gamma-aminobutyric acid, theanine, blueberry powder and various prebiotics and probiotics are combined for use, so that field planting and proliferation of the probiotics after intake can be guaranteed, intestinal health can be protected, the requirement for sleep improvement can be met, anxiety can be relieved, safety and mildness are achieved, and dependence is avoided.

The invention belongs to the technical field of biological bacterial fertilizer, and particularly relates to a compound microbial agent. The compound microbial agent is prepared from five soil beneficial bacteria and three synergists, wherein bacillus licheniformis (TMQD01), bacillus megatherium (TMQJ05), silicate bacterium (TMQJ01), rhodopseudomonas palustris (TMQZ08) and lactobacillus cased (TMQR01) are mixed according to the effective viable counts being (0.8-1):(0.8-1):(2.5-3):(1.0-1.2):4, and it is ensured that the viable count is not smaller than 3 billion / ml; organic acid, water solubleamino acid, gamma-polyglutamic acid and other synergists, solid phosphorus and potassium in soil are dissolved, available phosphorus and potassium in soil are increased, the purpose of dissolving phosphorus and potassium is achieved, the photosynthetic effect rate is increased, plant fructification is promoted, crop disease resistance is improved, and the effect of reducing agricultural capital input is achieved.

The invention discloses a chicken feed, and belongs to the technical field of poultry feeds. The chicken feed is prepared from the following raw materials in parts by weight: 65 parts of corn, 24 parts of soybean meal, 3 parts of fish meal, 0.7 part of dicalcium phosphate, 0.3 part of phytate phosphorus, 0.715 part of shell powder, 0.185 part of an additive, 0.4 part of sodium sulfate, 0.3 part of table salt, 0.4 part of astragalus membranaceus multi vitamin, and 5 parts of a green feed, wherein the green feed is one or more of pasturage, pine needle powder and herba euphorbiae thymifoliae; the additive is the mixture of stone powder, choline chloride, methionine and lysine. The feed can meet various nutritional requirements for the growth and the development of chickens.

The invention discloses an improved formulation of blood agar culture medium, made from tryptone, beef extract powder, liver digest, yeast extract, starch, sodium chloride, defibered sheep blood and agar, wherein each 1000 ml of the culture medium contains 10-20 g of tryptone, 3-8 g of beef extract powder, 1-4 g of liver digest, 0.5-2 g of starch, 3-8 g of yeast powder, 3-8 parts of sodium chloride, 40-60 ml of defibered sheep blood, 10-20 g of agar, and has pH of 7.2+ / -0.2. The improved formulation can provide complete nutrition, maintain osmotic pressure for bacteria, and repair the damaged bacteria. The formulation optimized herein may meet the complete demands of common bacteria and most fastidious bacteria, and is good for the cultivation and identification of bacteria.

The invention relates to a composite pig blood anticoagulant and a use method thereof. The composite pig blood anticoagulant comprises sodium citrate, ethylene diamine tetraacetic acid, sodium acetate and sodium chloride at the weight ratio of 6:4:1:3. Before being used, the composite pig blood anticoagulant needs to be dissolved into water, and each 56g of the pig blood anticoagulant needs to be dissolved into 1L of water, and correspondingly the mixture is added into 12kg of pig whole blood. The composite pig blood anticoagulant provided by the invention can ensure that pig blood does not be subjected to obvious clotting phenomenon within four days at normal temperature. The composite pig blood anticoagulant is simple in formula and convenient in operation, is economical and applicable, and can be used for effectively preventing the pig blood from clotting with reduced environmental pollution.

The invention relates to a water-retaining agent and a preparation method thereof, in particular to a water-retaining agent for meat products and aquatic products and a preparation method thereof, belonging to the technical field of food additives. The water-retaining agent is formulated from the following raw materials in parts by weight: 20-50 parts of whey protein, 20-30 parts of potassium carbonate, 10-20 parts of potassium citrate, 10-15 parts of sodium bicarbonate and 10-15 parts of glucono-delta-lactone. The phosphorus-free water-retaining agent can be used for meat products and aquatic products. With the adoption of the phosphorus-free water-retaining agent, the weight and water binding capacity of the products during soaking, cold storage, thawing and the like can be significantly improved, the yield of the products is improved, and water loss of the products during processing, cold storage, thawing can be prevented. The phosphorus-free water-retaining agent is free of phosphorus, so that the problems that the content of phosphate exceeds a standard, unpleasant metal smell is produced after the products are cooked, the texture of the products is coarse and the like due to addition of phosphate water-retaining agents can be avoided.

The invention relates to a combined medicament for supplementing zinc and calcium and a preparation method thereof. Medicament components are released at a fixed part. The preparation method comprises the following steps of: taking zinc gluconate and vitamin D, adding medicinal starch and microcrystalline cellulose and mixing, adding crushed superfine silica gel powder and evenly mixing and sub-packaging into enteric capsules; and mixing water-soluble calcium acetate, the medicinal starch and the microcrystalline cellulose, adding the crushed superfine silica gel powder and evenly mixing and sub-packaging into Muslim capsules. The medicament and the preparation method thereof ensure that the vitamin D cannot be damaged in an acid environment, avoid the direct stimulation of the zinc gluconate to the stomach and block off the direct contact of vitamin E and oxygen. After patients take two kinds of capsules, effective components of the vitamin E and the calcium acetate are rapidly disintegrated and absorbed in the stomach; the vitamin D and the zinc gluconate are disintegrated and absorbed in the intestine; and the invention has the characteristics of taking effect of medicament, high bioavailability, small side effect and the like, and is specially suitable for children, pregnant women, adults and old people for supplement and administration for a long term.

A food for supplementing estrogen to human body and regulating the intenstinal bacilli is prepared from soybean isoflavone, bifidobacteria, lactolin and VC1.

The invention discloses an anti-stress premix for feeder cattle. The anti-stress premix comprises montmorillonite, manyprickle acanthopanax root, atractylodes, radix astragali, radix scrophulariae, radix bupleuri, liquorice and a stomach-invigorating component, wherein the stomach-invigorating component is a mixture of sodium sulfate, sodium bicarbonate, sodium chloride and potassium sulfate or a traditional Chinese medicine of stomach-invigorating powder. The premix provided by the invention is added in the feed for the feeder cattle without being destroyed, can be absorbed by the feeder cattle effectively, and can play a role of resisting stress.

The invention relates to a large-scale production method for clostridium butyricum. The method comprises the following steps: (1) preparing a clostridium butyricum suspension with clostridium butyricum colonies of slant culture; (2) inoculating the clostridium butyricum suspension into fermentation broth in a seed tank, and performing anaerobic culture to a logarithmic growth phase to obtain fermentation strains; and (3) inoculating fermentation strains into the fermentation broth in a fermentation tank, and performing large-scale anaerobic fermentation production to obtain a clostridium butyricum product with a living bacteria number greater than 10<8>CFU / ml. The method prepares the clostridium butyricum suspension with a clostridium butyricum lawn on a slant culture medium, and then inoculates the clostridium butyricum suspension into the seed tank for anaerobic fermentation, and experimental results show that the same fermentation effect as a clostridium butyricum fermentation seedsolution can be achieved; the method does not require secondary expanding culture, so that the fermentation cycle is shortened by at least one primary seed fermentation cycle in the entire process flow; and meanwhile, at least one strain passage is also reduced, thus reducing the risk of strain variation caused by excessive strain passage.

The invention discloses a separating mediumon solution for removing dead hepatocytes and a method for removing dead hepatocytes by utilizing the separating mediumon solution and belongs to the technical field of hepatocyte isolationliver cell separation and purification. The separating mediumon solution is prepared by adding the following components in a William's E culture medium: 0.1-1.5 percentv / v of ITS universal culture additives, 1.5-2.5 mmol / L of L-glutamine, 1-20 [mu]gmu g / L of an epidermal growth factor, 15-20mg / L of hydrocortisone, 35-45 [mu]gmu g / L of dexamethasone, 0.5-1 percent v / v of penicillin streptomycin, 1-10 percent v / v fetal calf serum and 0.25-0.5g / mL of Nycodenz. The separating medium tion solution has the advantages of maintaining normal osmotic pressure of hepatocytes, repairing partial damaged hepatocytes and providing nutritional requirement for hepatocytes.

The invention relates to a treating fluid used for the cultivation of nucleus pearls, and belongs to the technical field of pearl cultivation. The treating fluid comprises the following components: (a),a first treating fluid and (b), a second treating fluid, wherein the first treating fluid comprises the components as follows: a bamboo charcoal powder solution, a dry-chrysanthemum solution, a gold mudstone solution, a gobi stone solution, a safflower parasitic skin solution, a peach parasitic skin solution, a safflower loranthus parasiticus skin solution and a vitamin B solution; the second treating fluid comprises the components as follows: acid red, chondroitin, mafenide, taurine, sodium chloride, hyriopsis cumingii polysacchride, vitronectin and water. According to the invention, through using the treating fluid for cultivation, the technical problems that the peals cultivated by the traditional method are dim in luster, poor in quality and low in survival rate are solved.

The invention relates to a method for cultivating nucleus pearls, and belongs to the technical field of pearl cultivation. The method comprises the steps as follows: step one, taking out a pearl nucleus to be put into a first treating fluid for soaking and cultivation; step two, taking out a cell chip of the edge membrane of the rear half part of a mussel body to be put into a second treating fluid for soaking and cultivation; step three, enabling the cell chip to be adhered to the pearl nucleus and planted into the mussel for cultivation so as to form a nucleus pearl, wherein the first treating fluid comprises the components as follows: a bamboo charcoal powder solution, a dry chrysanthemum solution, a gold mudstone solution, a gobi stone solution, a safflower parasitic skin solution, a peach parasitic skin solution, a safflower loranthus parasiticus skin solution and a vitamin B solution; the second treating fluid comprises the components as follows: acid red, chondroitin, mafenide, taurine, sodium chloride, hyriopsis cumingii polysacchride, vitronectin and water. According to the method provided by the invention, the rejection of the mussel body to pearl nucleus is overcome, the vitality of the cell chip is promoted, and large-scale operation can be implemented.

The invention discloses a primary hepatocyte cryopreservation solution, a hepatocyte cryopreservation method and a hepatocyte recovery method, and belongs to the field of primary hepatocyte cryopreservation and recovery. The primary hepatocyte cryopreservation solution comprises, by volume, 45% of a solution A, 10-45% of fetal bovine serum, 10% of dimethyl sulfoxide, and 0-35% of water, and the solution A comprises D-glucose, hydroxyethyl starch, lactobionic acid, polyvinylpyrrolidone, D-raffinose pentahydrate, potassium hydroxide, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, adenosine, reduced glutathione, allopurinol, tauroursodeoxycholic acid, and diammonium glycyrrhizinate. The invention also discloses the hepatocyte cryopreservation method and the hepatocyte recovery method. The primary hepatocyte cryopreservation solution can reduce the damages of the primary hepatocytes in the cryopreservation process and improve the vitality and adherence efficiency of the primary hepatocytes after thawing, and can be widely used in basic research and new drug development for livers.

The invention provides a mesenchymal stem cell preparation. The preparation comprises mesenchymal stem cells, human serum albumin, low molecular heparin, compound amino acids, vitamin C and a compoundelectrolyte. The mesenchymal stem cells are processed by inflammatory cell factors. The mesenchymal stem cell preparation is suitable for relieving inflammation and repairing injured tissue in rheumatoid arthritis so as to realize the symptom relieving and function restoring of a patient.

The invention discloses a placental tissue preservation liquid and a preparation method thereof. The placental tissue preservation liquid includes glucose injection, a serum replacement, gentamicin, amphotericin, trehalose and heparin lithium. The preparation method of the placental tissue preservation liquid includes adding the serum replacement, gentamicin, amphotericin B, magnesium ascorbyl phosphate hydrate, trehalose and heparin lithium into the glucose injection, wherein final concentrations of the ingredients are 0.5-2%, 800 U / mL, 15-30 ug / mL, 0.25-0.5 mg / mL, 0.6-3 mg / mL, and 20-40 U / mL. The placental tissue preservation liquid has clear composition and balanced nutrition, has no evident toxic and side effects on cells, can well maintain activity of in-vitro placental cells, and iseffective in preventing contamination of exogenous microorganisms introduced in the acquisition and transport steps.

The invention relates to the field of sperm freezing, and particularly relates to a sperm freezing medium as well as a preparation method and application thereof. The sperm freezing medium comprises the following components: potassium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, dipotassium phosphate, sodium pyruvate, calcium lactate, glucose, saccharose, glutamine, glycine,HEPES, gentamicin, glycerinum, human serum albumin, vitamin E, vitamin C and vitamin B12. According to the sperm freezing medium, all components are matched with each other to comprehensively maintainthe osmotic pressure, the straining capacity of sperm to resist the outside environment change; the freezing medium is used for storing sperm in low temperature; the sperm survive rate and the activity can be maintained for a long term.

The invention discloses a later-stage compound feed capable of improving somatotype and meat quality of fattening pigs and a processing method of the later-stage compound feed, and belongs to the technical field of feeds. The later-stage feed is prepared from the following raw materials in percentage by mass: 0.2-0.4% of betaine, 0.02-0.05% of chromium picolinate, 0.01-0.02% of selenium yeast, 0.01-0.02% of vitamin E, 0.01-0.03% of vitamin C, 0.06-0.10% of magnesium sulphate, 0.1-0.2% of potassium chloride, 64.0-66.0% of corn, 20.0-22.0% of soybean meal, 5.0-6.0% of oil bran, 0.8-1.0% of palmoil, 4.0-6.0% of coarse bran, 0.38-0.50% of calcium hydrogen phosphate, 1.05-1.20% of stone powder, 0.40-0.45% of sodium chloride, 0.05-0.07% of an antioxidant and 1.0% of a premix.

The invention belongs to the field of waste water biological strengthening treatment, and concretely relates to an activation promoter capable of increasing microbiological treatment effect of daily chemical waste water. The daily chemical waste water has the characteristics of high organic matter, high salt and biological toxicity, and treatment by using a conventional biology method is difficultto reach the standard. The activation promoter comprises chitosan, glycine betaine, ferrous sulphate, trehalose, citric acid, proline, and composite vitamin, can effectively improve the survival breeding capability and metabolic activity of microbe under conditions of high organic matter and high salt, and can effectively increase the sewage processing effect. The activation promoter has the advantages of low cost and convenient making, and can be used for biological treatment of daily chemical waste water and other high-salt surfactant waste water.

The invention discloses a novel preservation solution for an alveolar lavage fluid microbial nucleic acid. The preservation solution comprises a treatment solution I and a treatment solution II, wherein the treatment solution I comprises a guanidine hydrochloride buffer solution, a nuclease inhibitor, a metabolism inhibitor, an alkali metal halide and glycerol, and the treatment solution II comprises an alkali metal hydroxide and a surfactant. The treatment solution I and the treatment solution II which are separately stored are used as the novel preservation solution for the alveolar lavage fluid microbial nucleic acid, so that the preservation solution can be prevented from generating precipitates, at the same time, different interference impurities can be treated respectively in a targeted manner, alveolar lavage fluid samples can be rapidly stabilized, the activity of nuclease is effectively inhibited, the cell lysis is prevented, at the same time, the free nucleic acid in the alveolar lavage fluid can be well protected, the preservation time is long, the accuracy and convenience of nucleic acid detection are improved, and thereby the preservation solution has great applicationvalue in the detection and diagnosis of tumor diseases, viruses and the like.

The invention discloses an industrial fermentation method of phytase. The method comprises the following steps: (1) fermentation in a first-level fermentation tank; (2) fermentation in a second-levelfermentation tank; (3) high-density fermentation; (4) fed-batch fermentation and starving; (5) induction and feed mixing. The industrial fermentation method of phytase disclosed by the invention has the characteristics of high tank enzyme activity and high fermentation efficiency.

The invention discloses a sperm diluent preparing method and belongs to the technical field of medical examination. A diluent prepared with the method contains substances such as glucose, citric acid, sodium citrate, yolk, glycerol, N-acetyl-L-cysteine, penicillin and the like. The diluent does not affect motility and quality of sperms and can shorten the semen liquidation time and the patient waiting time, detection of indexes such as the number, the motility, the malformation condition and the like of the sperms can also be performed on a patient whose semen is not liquidized, and the motility of the sperms can be kept for a long time. The detection method which is simple in pretreatment, quick, sensitive, cheap and capable of realizing long-acting preservation is applicable to conventional detection of the semen.

The invention relates to the field of fresh keeping of seafoods, in particular to a fresh keeping method of seafoods. The fresh keeping method of the shellfish seafoods comprises the steps of placingfresh seafoods to a heat-insulation container loaded with an edible salt solution of minus 2 to 2 DEG C, and placing the heat-insulation container to be environment of minus 3 to 0 DEG C. According tothe fresh keeping method of the seafood, the fresh keeping time of the seafoods can be prolonged to 15 days or above, the economic value of the seafoods increased, and the fresh keeping method has high promotion value.