125 results about "Lavage fluid" patented technology

The present invention relates to closed catheter suction systems used to aspirate secretions from the trachea of a patient and to rinse the catheter after such aspiration. The apparatus comprises a flexible suction catheter encased within a sheath that is fixed to a vacuum source at its proximal end and extendable through an adapter assembly at its distal end, near the patient. The adapter assembly connects the closed catheter suction system to a patient connector, and a suction control valve at the proximal end operates to aspirate secretions and to rinse the catheter. The closed catheter suction system also includes a lavage port and a rinse port, the latter of which is positioned near the distal end and is isolated from the ventilation circuit through the innovative use of a rinse chamber and a one-way valve. Advantageously, the lavage port is positioned near the proximal end, away from the patient, so as to prevent the accidental leakage of lavage into the ventilation circuit. Further, both the lavage port and the rinse port include a self-closing fill-valve that prevents the accidental loss of ventilator volume and which also prevents the discharge of infectious pathogens to the surrounding environment throughout the aspiration and rinse procedures.

A portable, lightweight bowel evacuation apparatus and method of delivering a colonic lavage is provided. The apparatus includes a liquid bag containing lavage liquid connected to a supply conduit that leads to a nozzle assembly. The apparatus also includes a drain conduit leading from the nozzle assembly to a waste collection. A pulsating member, such as a manual pump or squeeze bulb is provided to generate pulsed waves in the lavage liquid in the patient's colon so as to break up and remove fecal material.

A bronchoalveolar lavage instillation / aspiration system which includes (i) a needleless bronchoalveolar lavage instillation / aspiration device with a barrel member including a barrel lumen having a distal aperture and a self-sealing member configured for maintaining a fluid-disruptable, resealable barrier to the distal aperture; and (ii) a catheter assembly configured to provide a patent path of fluid communication between the instillation / aspiration device and a patient bronchial passage, where the catheter includes a wedging structure configured to engage a patient bronchial passage. In another aspect, embodiments of the present invention may include one or more methods of making and using a system or component described herein.

A tracheal tube has a sealing cuff towards its patient end and a suction aperture just above the cuff. The suction aperture connects via a lumen with a suction line terminated by a connector. One end of the connector is tapered and has a port for connection to a suction source. The connector also has a side port in which the nose of a syringe can be inserted to inject a lavage fluid out of the suction aperture to help loosen secretions. When not in use, the side port is closed by a valve or by a closure cap with formations for sealing the two ports.

Colorectal lavage device including a drain assembly, and a colonic tube assembly including an inflow tube, a flow control mechanism to control flow through the inflow tube, an outflow tube and a flow control mechanism to control flow through the outflow tube. The drain assembly has a tubular body defining an interior space and having a proximal portion insertable into an anus of a person to cause the anus to constrict around the proximal portion and thereby seal the proximal portion against the anal wall, a distal portion including a distal end having an opening in line with an opening in the proximal portion and a side tube arranged at an angle to the proximal portion. The inflow tube passes through the tubular body and has a tip with one or more apertures through which the lavage fluid operatively flows. The outflow tube is connected to the side tube.

A hand-held disposable body cavity irrigation device comprises a pump with an inlet and an outlet, a container for lavage fluid fastened removably to said pump, an applicator having an insertion tip, and divided longitudinally into an injection chamber with a first fitting and a drain chamber with a second fitting, and a first tube connecting said outlet to the first fitting wherein the first tube comprises at least one malleable element embedded longitudinally into an elastic wall, said inlet communicates with the lavage fluid within the container, and the drain chamber is disposed predominantly below the injection chamber and has at least one vent beyond the insertion tip.

The invention relates to a kit for detecting novel coronavirus N protein and an application of the kit. The kit comprises (1) one antibody capable of being bound to the novel coronavirus N protein, and (2) the other antibody marked by a marker and capable of being bound to the novel coronavirus N protein when the novel coronavirus N protein can be bound to (1) to limit the antibody. The using method of the kit comprises the step of determining whether a patient suffers from the novel coronavirus pneumonia or not by detecting the level of the novel coronavirus N protein in an individual sample.The kit for detecting the novel coronavirus N protein provided by the invention can be used for detecting the coronavirus N protein in nasopharynx swabs, oropharynx swabs, alveolar lavage fluids, serum, plasma, whole blood, sputum, oral swabs or saliva samples so as to be used for diagnosing novel coronavirus pneumonia.

The invention relates to an immunochromatographic kit for rapidly detecting novel coronavirus N protein as well as a preparation method and application of the immunochromatographic kit. The immunochromatographic kit comprises a test strip, the test strip comprises a detection line and a quality control line, the detection line is coated with an anti-novel coronavirus N protein antibody, and the quality control line is coated with a goat anti-rabbit polyclonal antibody. The immunochromatographic kit for rapidly detecting the novel coronavirus N protein provided by the invention can be used fordetecting the novel coronavirus N protein in a nasopharynx swab, an oropharynx swab, an alveolar lavage fluid, an oral swab or a saliva sample so as to be used for diagnosing novel coronavirus pneumonia.

A hand-held disposable body cavity irrigation device comprises a pump with an inlet and an outlet, a container for lavage fluid fastened removably to said pump, an applicator having an insertion tip, and divided longitudinally into an injection chamber with a first fitting and a drain chamber with a second fitting, and a first tube connecting said outlet to the first fitting wherein the first tube comprises at least one malleable element embedded longitudinally into an elastic wall, said inlet communicates with the lavage fluid within the container, and the drain chamber is disposed predominantly below the injection chamber and has at least one vent beyond the insertion tip.

The invention provides a method for extracting and cryopreserving hematopoietic stem cells taking a placenta as a source. The extraction method comprises the following steps: cleaning and detecting collected placenta and maternal blood; repeatedly performing lavage on the placenta detected to be qualified by using lavage fluid so as to obtain lavage fluid containing the hematopoietic stem cells; and concentrating and purifying the extracted lavage fluid containing the hematopoietic stem cells, thereby obtaining the hematopoietic stem cell population. The lavage method adopted in the hematopoietic stem cell extraction process in the invention is simple in equipment and convenient to operate, and the closed lavage system can effectively avoid infection of bacteria in air. According to the lavage method, the recovery rate of the extracted hematopoietic stem cells is extremely high, and waste of resources is effectively avoided. A programmed temperature control manner is adopted in the cryopreservation process of the hematopoietic stem cells, and the corresponding cryopreservation rate is set according to temperature rise and drop rules in the cryopreservation process of the hematopoietic stem cells, so that the hematopoietic stem cells can be prevented from being damaged to the greatest degree.

The invention discloses lactobacillus reuteri capable of alleviating allergic asthma and application thereof and belongs to the technical field of medicines. The lactobacillus reuteri has an effect ofrelieving allergic asthma, which is embodied by: (1) significantly reducing lung inflammation in mice with allergic asthma; (2) significantly inhibiting the generation of dust mite specific immunoglobulin IgG1 in serum of allergic asthma mice; (3) significantly reduced the contents of IL-5 and IL-13 in alveolar lavage fluid of mice with allergic asthma; (4) significantly reducing the content of IL-17A in alveolar lavage fluid of mice with allergic asthma, and thus the lactobacillus reuteri has great application prospects in the preparation of products for preventing and / or treating allergic asthma.

Compositions, methods and kits to be used as a lubricant and for treatment of a cavity pathology including joint pathology are provided. The composition comprises therapeutically effective amounts of at least one bioactive agent, such as magnesium compound, at least one lubricating agent, such as hyaluronic acid, and at least one cell membrane repairing agent, such as polyethylene glycol. The components of these compositions may be administered by a direct application, an application through a cannula, an intra-articular injection, as a flush fluid during an arthroscopy of the affected area, as a post-arthroscopy injection, or as part of a lavage of the area affected by the intra-articular pathology. In addition, the composition of the present invention may be administered to the patient from a pump or a depot.

Method for gentle separation of viable sporadic cells from body fluids such as blood, from malignant effusions, bronchoalveolar lavage fluid, peritoneal lavage fluid and amniotic fluid, based on a filter membrane which is in an intimate contact with an absorbent material. Using the present method it is possible to isolate for example circulating and disseminated tumor cells, endometrial cells and circulating trophoblast cells, allowing subsequent detection, quantification, characterization and especially culturing of said cells. An apparatus for carrying out the method is further disclosed.

A system for anal and / or stomal irrigation comprises a reservoir (102) for an irrigating liquid, a catheter (100) comprising a catheter tip for insertion into the rectum or stoma of a user and for expelling of the irrigating liquid from the catheter tip, and a tubing system (121) providing a conduit for the irrigating liquid between the reservoir and the catheter tip. A pump (101) is provided to pump the irrigating liquid from the reservoir to the catheter tip, and a control system (103) controls a flow condition, such as the amount of liquid expelled at the catheter tip. A sensor (153) determines a measure of pressure in at least one first predetermined position in the tubing system and / or the catheter during operation of the pump, on the basis of which the flow condition is estimated. The control system is configured to control the pumping operation of the pump in response to the measure of pressure.

A diagnostic test and method is provided comprising mixing blood or another biological fluid sample with a test compound and spotting the blood on filter paper for subsequent analysis of the effect of the test compound on the blood. The biological fluid can be a cerebrospinal fluid, a peritoneal fluid, a cyst fluid, an amniotic fluid, a lavage fluid, a saliva, a cell extract or a tissue extract. The compound is chosen among an amino acid, a peptide, a protein, a carbohydrate, an oligosaccharide, a polysaccharide, a glycoprotein, a lipid, a lipoprotein, a glycosaminoglycan, a hormone, a steroid, a vitamin, a low molecular weight synthetic or natural compound which influences the blood to cause an alteration of its composition, e.g., a toxin, allergen, autoantigen, bacterial protein or polysaccharide, viral protein, fungal protein or polysaccharide, parasitic protein or polysaccharide, bacterial lipopolysaccharide or any other compound relevant to diseases.

The invention provides a kit for detecting SARS-CoV-2 novel coronavirus nucleic acid at a constant temperature by using an enzyme digestion probe. The kit comprises an isothermal amplification primerfor detecting an ORF1ab gene of SARS-CoV-2 novel coronavirus and a corresponding RNA-containing base probe capable of being digested by RNase H, wherein the left end and the right end of an RNA base of the base probe are respectively marked with a reporter fluorophore and a quencher. By utilizing the efficient isothermal amplification primer and the probe, constant-temperature multiple detection can be carried out on the SARS-CoV-2 novel coronavirus, and the kit has high sensitivity and high specificity. The novel coronavirus nucleic acid detection kit disclosed by the invention has high sensitivity and specificity, can be used for detecting throat swabs, alveolar lavage fluid and sputum samples of suspected novel coronavirus pneumonia cases, suspected aggregated case patients and other patients needing novel coronavirus infection diagnosis or differential diagnosis, and has the characteristics of simplicity, convenience, quickness and accuracy.

A safe, simple, convenient, low-pollution and good-repeatability extracting method of alveolar lavage fluid metagenome DNAs is provided. The method includes steps of liquefying a sample, crushing cells, isolating target DNAs, removing impurity proteins and salt ions, performing DNA adsorption and performing DNA elution. The method can increase the yield and purity of the metagenome DNAs and can meet PCR, DNA library construction, gene sequencing, and other molecular biological experiments.

The invention discloses an allergen protein BA2 of Periplaneta americana and an expression method thereof. The expression method comprises the following steps: (1) extracting the RNAs of Periplaneta americana, subjecting the RNAs to inverse transcription to obtain cDNAs and carrying out PCR amplification with the cDNAs as a template so as to obtain a coding gene fragment of the allergen protein ofPeriplaneta americana; and (2) cloning an allergen gene into a prokaryotic expression vector to construct recombinant expression plasmid pET28a(+)-BA2, transferring the recombinant expression plasmidinto the host bacterium Escherichia coli BL21, inducing the expression of the recombinant expression plasmid with IPTG and carrying out purifying by using affinity chromatography so as to obtain therecombinant allergen protein BA2 of Periplaneta americana. The results of pharmacological experiments show that the allergen protein BA2 can obviously increase the IgE content (wherein P is less than0.05) of mouse serum, substantially increase the contents of IL-4, IL-5 and IL-13 inflammatory factors in mouse bronchoalveolar lavage fluid and mouse splenocyte supernatant, and result in obvious pathological changes of mouse lung tissue. The method provided by the invention can prepare the allergen protein with high purity and provides referential bases for subsequent research on the allergens of Periplaneta americana and potential allergen substances in other animals.

The invention discloses a new application of nucleotide and an application of the nucleotide in preparation of drugs for preventing and treating diseases such as allergic rhinitis or functional food for relieving symptoms of the allergic rhinitis. The nucleotide is a mixture of five nucleotides, namely CMP, AMP, UMP, GMP and IMP. The molecular weight of the nucleotide is small, absorption of the nucleotide taken by a human body is fast, and bioavailability of the nucleotide is high. It is found that the ingestion of the nucleotide can relieve the symptom of allergic rhinitis of mice, relieve splenomegaly caused by allergy, significantly reduce the histamine level in serum and nasal cavity lavage fluid, and effectively regulate inflammatory factors, thereby playing a role in treating allergic diseases. The nucleotide can be used for preparing medicines for preventing and treating diseases such as allergic rhinitis or functional foods for relieving symptoms of allergic rhinitis, and provides a new way for regulating the immune function in a diet intervention mode.

The invention discloses a multi-amplification and high-throughput sequencing method and kit for identifying pathogenic microorganisms, and the method comprises the following two steps: in the first-step amplification, an upstream long primer pool is used for single-end amplification; in the second-step amplification, a downstream long primer pool, a primer P1 and a primer P2 are added into a reaction system, the three primers form a primer for the second-step amplification, and then quality control, purification, high-throughput sequencing and data analysis are carried out. According to the method, the pathogenic microorganisms which cannot be identified in a traditional clinical method are detected in a human respiratory tract alveolar lavage fluid sample and a blood or plasma sample by using a two-step multiple amplification and next-generation sequencing method for the first time, the sensitivity is high, the detection limit is 50 pathogenic genome copies per milliliter, the method is rapid and simple, the sequencing data volume is small, and the detection cost is reduced.

The invention discloses a method for preparing a pneumonia rat model and a model evaluation method, and belongs to the technical field of biology. The method for establishing the pneumonia rat model is non-invasive and safe to rats, does not damage other organs of rats in the whole process, and effectively avoids the death of the rats. Besides, the method has simple operation and high success rate. According to the pneumonia rat model established by the method, pseudomonas aeruginosa in bronchoalveolar lavage fluid is obviously increased, inflammatory factors IL-4 and TNF-alpha are obviously increased, the level of adhesion factor IFN-gamma is obviously reduced, and the number of leukocytes is obviously increased. Meanwhile, the lung index is also obviously increased, which indicates thatthe model is established well.

The present invention provides a method of raising livestock, which can improve an efficiency of propagating or rearing without using a drug such as an antibiotic or a disinfectant. The method uses a lactic acid bacterium (such as Enterococcus faecium or Lactobacillus fermentum) proliferated and cultured in a medium containing a nutrient source derived from a plant. Specifically, a uterus of a domestic animal after delivery is washed with a lavage fluid containing the lactic acid bacterium as an active ingredient; the lactic acid bacterium is orally administered to a baby domestic animal suffering from diarrhea during the lactation period; and tap water containing the lactic acid bacterium is sprayed over the interior of the livestock house to condition the humidity. Thus, the lactic acid bacterium is totally used in breeding and growing, whereby healthy condition of a domestic animal is maintained, resulting in being capable of producing well-grown meet with good quality and good taste. The meat is safe because it has no adverse affect on the human body due to a residual harmful substance or the like.

The invention discloses a balloon catheter assembly for bronchoscope. The balloon catheter assembly comprises a hairbrush for brush biopsy, an inner catheter used for conveying lavage fluid, medicinefluid and the hairbrush from the external portion to a target bronchus, and an outer catheter which sleeves the inner catheter and is provided with a balloon. The outer catheter is in sealing connection with the inner catheter so that a sealed space can be formed between the inner catheter and the outer catheter, and the balloon can be inflated, wherein the outer catheter is communicated with an air inflation pipe. The inner catheter is provided with a lavage channel, a medicine injection channel and a hairbrush send-in channel, wherein two ends of the lavage channel are penetrating, the lavage channel is used for conveying the lavage fluid, the medicine injection channel is used for conveying the medicine fluid, and the hairbrush send-in channel is used for conveying the hairbrush. An anti-polluting plug used for blockwork is arranged at the outlet end of the hairbrush send-in channel. The balloon catheter assembly is used for bronchoalveolar lavage, medicine injection and hairbrush biopsy of the bronchus, improves the treatment efficiency and reduces the economical burdens of patients.

The invention provides a sample harvester of bronchoalveolar lavage fluid, which comprises a fluid collecting bottle, a first pipeline, a second pipeline, a first buckling cover and a second bucklingcover; there are multiple liquid collecting bottles, and each liquid collecting bottle is detachably and hermetically mounted at the bottom of the first buckling cover through a bottle opening of theliquid collecting bottle; the first pipeline comprises a main pipeline and a plurality of branch pipelines, and the main pipeline is communicated with a plurality of branch pipelines; the first buckling cover is provided with first through holes and a second through hole, the number of the first through holes and the liquid collecting bottles are the same; each branch pipeline is connected with one first through hole, and the second buckling cover is connected with the first buckling cover, and the second buckling cover can move relatively to the first buckling cover; a third through hole is formed in the second buckling cover, and when the second buckling cover and the first buckling cover move relatively, the third through hole can be communicated with each second through hole; one end of the second pipeline is connected with the third through hole. The sample harvester can collect multi-tube bronchoalveolar lavage fluid samples in one time without replacing tubes, the safety is extremely high, and meanwhile the operation is easy and convenient.

The invention relates to a real-time fluorescence RPA detection kit for aspergillus fumigatus as well as a special primer and a probe of thereof, and particularly provides a primer composition for RPAdetection, the primer composition comprises a forward primer and a reverse primer, the forward primer has a nucleotide sequence shown as SEQ ID NO: 1, and the reverse primer has a nucleotide sequenceshown as SEQ ID NO: 2. The primer composition for RPA detection can be used for rapidly, conveniently, efficiently, specifically and sensitively detecting aspergillus fumigatus components in samplesto-be-detected, such as thalli, sputum, blood and bronchoalveolar lavage fluid, under an isothermal condition.

The invention relates to application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction, and belongs to the technical field of microorganisms and medicines. According to the application disclosed by the invention, the Faecalibacterium prausnitzii A2-165 is applied to relieving of allergic asthma and rhinitis, and the application is specifically embodied in that(1) the infiltration effect of memory inflammatory cells with lung pathological symptoms of mice with allergic asthma and rhinitis is remarkably improved; (2) the contents of IL-4, IL-5 and IL-13 in alveolar lavage fluid for the mice with allergic asthma and rhinitis are remarkably reduced; (3) the generation of dust mite specific immunoglobulin IgG1 in serum of the mice with allergic asthma and rhinitis is remarkably inhibited; and (4) the ratio of Tregs in the spleen is remarkably increased, so that the Faecalibacterium prausnitzii A2-165 has a huge application prospect in the preparation ofa product for prevention and / or treatment.

The invention discloses a novel preservation solution for an alveolar lavage fluid microbial nucleic acid. The preservation solution comprises a treatment solution I and a treatment solution II, wherein the treatment solution I comprises a guanidine hydrochloride buffer solution, a nuclease inhibitor, a metabolism inhibitor, an alkali metal halide and glycerol, and the treatment solution II comprises an alkali metal hydroxide and a surfactant. The treatment solution I and the treatment solution II which are separately stored are used as the novel preservation solution for the alveolar lavage fluid microbial nucleic acid, so that the preservation solution can be prevented from generating precipitates, at the same time, different interference impurities can be treated respectively in a targeted manner, alveolar lavage fluid samples can be rapidly stabilized, the activity of nuclease is effectively inhibited, the cell lysis is prevented, at the same time, the free nucleic acid in the alveolar lavage fluid can be well protected, the preservation time is long, the accuracy and convenience of nucleic acid detection are improved, and thereby the preservation solution has great applicationvalue in the detection and diagnosis of tumor diseases, viruses and the like.

The invention belongs to the field of gene diagnosis, and relates to application of a detection reagent using methylation of HOXA7 as a detection object to preparation of a lung cancer diagnosis reagent. The detection reagent uses HOXA7 as a tumor marker for detecting the lung cancer, reaches high sensitivity of 88.6% and high specificity of 95% in sputa, reaches high sensitivity of 76.2% and highspecificity of 95% in lavage fluid, and is higher in detection sensitivity than existing reported tumor markers of the lung cancer; particularly for adenocarcinomas, sensitivity of the detection reagent is greatly promoted; and the detection reagent has great application value for detection and diagnosis on the lung adenocarcinomas.

The present invention relates to the technical field of Chinese medicine, in particular to a traditional Chinese medicine composition and the use thereof The traditional Chinese medicine composition comprises the following ingredients: pear juice, greenish lily bulb juice, fig juice, water chestnut juice, grosvenor momordica fruit, southern almond and northern almond. The traditional Chinese medicine composition has significant promotions on the phlegm-reducing and cough-relieving capabilities of the rats, and on the immune indices of bronchoalveolar lavage fluid of the rats, exhibiting that the traditional Chinese medicine composition provided in the present invention has good effects of clearing heat and moistening the lungs.