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Application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction

A technology for allergic asthma and Clostridium prazines, applied in the field of microorganisms, can solve the problems of slow effect, unclear effect relationship, poor effect, etc.

Active Publication Date: 2021-02-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Probiotic preparations are safe and healthy for the human body, and the administration method is simple, but the currently discovered probiotics such as Lactobacillus adderiformis, Lactobacillus rhamnosus and Bifidobacterium animalis found to be effective in relieving symptoms of allergic asthma and rhinitis. The effect is not clear, and most of the probiotic preparations on the market also have the defect of slow effect, or the defect of unclear effect relationship
[0007]Therefore, there is an urgent need to find a kind of probiotics with strong pertinence to allergic asthma and rhinitis and strong ability to relieve allergic asthma and rhinitis to solve the existing problem of allergic asthma The probiotic preparations for rhinitis are slow to take effect and the relationship between the effects is not clear

Method used

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  • Application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction
  • Application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction
  • Application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the cultivation of Clostridium prausniformis

[0053] Specific steps are as follows:

[0054] After inserting Faecalibacterium prausnitzii A2-165 into YCFA solid medium (containing 0.05% cysteine) and culturing at 37°C for 48 hours, the colonies were observed and found to be convex, translucent, with complete edges, moist.

[0055] Insert Faecalibacterium prausnitzii A2-165 into YCFA liquid medium (containing 0.05% cysteine) and culture it anaerobically at 37°C for 18 h, then transfer to fresh YCFA liquid medium (containing 0.05% cysteine Cystine), cultivated under the same conditions for 18 hours, obtained the bacterial sludge by suction filtration in the anaerobic workstation, then washed and resuspended with 0.1M PBS (pH 7.2, containing 0.05% cysteine) to obtain the bacterial suspension, and used a sealed Store the bacterial suspension in a freeze-dried vial with a stopper and use it on the same day.

Embodiment 2

[0056] Example 2: Effects of Flostridium prausiticus on pulmonary pathology of allergic asthma and rhinitis mice

[0057] Specific steps are as follows:

[0058] 40 SPF grade female BALB / c mice (14-16g) were randomly divided into 5 groups, namely blank group, model group and experimental group, wherein the experimental group included supernatant group, dead bacteria group and live bacteria group, 8 in each group. The mice were fed with common feed in the Experimental Animal Center of Jiangnan University, with a constant temperature of 21-26°C, humidity of 40-70%, noise less than or equal to 60dB, and animal illumination of 15-20LX (all animal experiment procedures were managed by the animal welfare and ethics of Jiangnan University committee to review and approve).

[0059] The experiment lasted for four weeks: the mice were adaptively fed for 5 days, and from the 6th day, the mice in the model group and the experimental group (except the blank group) were anesthetized every...

Embodiment 3

[0063] Example 3: Effects of Clostridium praustrii on the number of eosinophils in the lungs of mice with allergic asthma and rhinitis

[0064] Specific steps are as follows:

[0065] 40 SPF grade female BALB / c mice (14-16g) were randomly divided into 5 groups, namely blank group, model group and experimental group, wherein the experimental group included supernatant group, dead bacteria group and live bacteria group, 8 in each group. The mice were fed with common feed in the Experimental Animal Center of Jiangnan University, with a constant temperature of 21-26°C, humidity of 40-70%, noise less than or equal to 60dB, and animal illumination of 15-20LX (all animal experiment procedures were managed by the animal welfare and ethics of Jiangnan University committee to review and approve).

[0066] The experiment lasted for four weeks: the mice were adaptively fed for 5 days, and from the 6th day, the mice in the model group and the experimental group (except the blank group) w...

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Abstract

The invention relates to application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction, and belongs to the technical field of microorganisms and medicines. According to the application disclosed by the invention, the Faecalibacterium prausnitzii A2-165 is applied to relieving of allergic asthma and rhinitis, and the application is specifically embodied in that(1) the infiltration effect of memory inflammatory cells with lung pathological symptoms of mice with allergic asthma and rhinitis is remarkably improved; (2) the contents of IL-4, IL-5 and IL-13 in alveolar lavage fluid for the mice with allergic asthma and rhinitis are remarkably reduced; (3) the generation of dust mite specific immunoglobulin IgG1 in serum of the mice with allergic asthma and rhinitis is remarkably inhibited; and (4) the ratio of Tregs in the spleen is remarkably increased, so that the Faecalibacterium prausnitzii A2-165 has a huge application prospect in the preparation ofa product for prevention and / or treatment.

Description

technical field [0001] The invention relates to the application of Clostridium praustrix in alleviating Th2 response of allergic asthma and rhinitis, and belongs to the technical field of microbes and the technical field of medicine. Background technique [0002] Allergic asthma and rhinitis (Allergic Asthma and Rhinitis) is a common clinical disease, refractory disease, the course of the disease is protracted and difficult to heal, and its main feature is the infiltration of various inflammatory cells (mast cells, eosinophils and T lymphocytes). Inflammatory mediators secreted by cells can amplify the inflammatory cascade, increase airway hyperresponsiveness (AHR) and stimulate mucus secretion, leading to airway obstruction and airway remodeling. In susceptible individuals, this inflammation can lead to recurrent episodes of wheezing, shortness of breath, chest tightness, and / or coughing, which can lead to life-threatening complications such as respiratory arrest and respir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P37/08A61P11/06A61Q11/02A23L33/135A23K10/18
CPCA61K35/741A61P37/08A61P11/06A61Q11/02A23L33/135A23K10/18A23V2002/00A23V2200/30A23V2200/314A23V2200/324A23V2200/32
Inventor 陈卫陆文伟胡文兵张秋香闫博文赵建新张灏
Owner JIANGNAN UNIV
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