Application of HOXA7 methylation detection reagent to preparation of lung cancer diagnosis reagent

Abstract

The invention belongs to the field of gene diagnosis, and relates to application of a detection reagent using methylation of HOXA7 as a detection object to preparation of a lung cancer diagnosis reagent. The detection reagent uses HOXA7 as a tumor marker for detecting the lung cancer, reaches high sensitivity of 88.6% and high specificity of 95% in sputa, reaches high sensitivity of 76.2% and highspecificity of 95% in lavage fluid, and is higher in detection sensitivity than existing reported tumor markers of the lung cancer; particularly for adenocarcinomas, sensitivity of the detection reagent is greatly promoted; and the detection reagent has great application value for detection and diagnosis on the lung adenocarcinomas.

Description

technical field [0001] The invention belongs to the field of gene diagnosis, and more specifically, the invention relates to the application of a human HOXA7 gene methylation detection reagent in the preparation of a lung cancer detection reagent, and a method for human HOXA7 gene methylation detection. [0002] technical background [0003] Lung cancer is a malignant tumor of the lung originating from the bronchial mucosa, glands or alveolar epithelium. According to the pathological type, it can be divided into: 1. Small cell lung cancer (SCLC): a special pathological type of lung cancer, with obvious tendency of distant metastasis and poor prognosis, but most patients are sensitive to radiotherapy and chemotherapy. 2. Non-small cell lung cancer (NSCLC): other pathological types of lung cancer other than small cell lung cancer, including squamous cell carcinoma, adenocarcinoma, large cell carcinoma, etc. There are certain differences in biological behavior and clinical cour...

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Problems solved by technology

(4) Bone scan: the sensitivity of detection of bone metastases of lung cancer is high, but there is a certain false positive rate

If an early tumor screening product cannot screen out all or most of the patients, those who miss the test will not be able to receive sufficient risk warnings, thus delaying the timing of treatment, which is a huge loss for patients loss

[0018] Although some tumor markers related to lung cancer have been found in the prior art, the sensitivity and specificity of these tumor markers cannot meet the demand due to the limitation of detection reagents or detection methods for these tumor markers. Therefore, the current There is still a need for further research in the field of screening methods that can actually be applied to lung cancer

However, although non-invasive screening has unique advantages in sampling, it also has some limitations in other aspects, such as adenocarcinoma in lung cancer, which is difficult to cough up through sputum due to exfoliated cells in the deep lung Generally speaking, those skilled in the art would think that this type of lung cancer is not suitable for non-invasive screening

On the other hand, even for other types of lung cancer, the currently reported non-invasive screening methods are difficult to meet the requirements of clinical use

Although relevant research has been progressing for many years, there is still no non-invasive screening method for lung cancer that can be promoted to the clinic

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