Method for extracting and cryopreserving hematopoietic stem cells taking placenta as source

Abstract

The invention provides a method for extracting and cryopreserving hematopoietic stem cells taking a placenta as a source. The extraction method comprises the following steps: cleaning and detecting collected placenta and maternal blood; repeatedly performing lavage on the placenta detected to be qualified by using lavage fluid so as to obtain lavage fluid containing the hematopoietic stem cells; and concentrating and purifying the extracted lavage fluid containing the hematopoietic stem cells, thereby obtaining the hematopoietic stem cell population. The lavage method adopted in the hematopoietic stem cell extraction process in the invention is simple in equipment and convenient to operate, and the closed lavage system can effectively avoid infection of bacteria in air. According to the lavage method, the recovery rate of the extracted hematopoietic stem cells is extremely high, and waste of resources is effectively avoided. A programmed temperature control manner is adopted in the cryopreservation process of the hematopoietic stem cells, and the corresponding cryopreservation rate is set according to temperature rise and drop rules in the cryopreservation process of the hematopoietic stem cells, so that the hematopoietic stem cells can be prevented from being damaged to the greatest degree.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting and freezing hematopoietic stem cells derived from placenta. Background technique [0002] Hematopoietic stem cells are the most primitive hematopoietic cells differentiated from totipotent mesenchymal cells in the yolk sac, and are the precursors of all blood cells in the body. Various blood cells, including red blood cells, white blood cells, and platelets, can also differentiate into other cells. Therefore, it is widely used in the treatment of hematological malignancies, severe aplastic anemia, some solid tumors, some abnormal immune diseases, some genetic diseases, metabolic diseases and very severe myeloid acute radiation sickness. At the same time, hematopoietic stem cell transplantation is also an effective means to restore normal hematopoietic and immune functions for cancer patients. [0003] According to research, the content of hematopoietic stem...

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Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for extracting and freezing hematopoietic stem cells from the placenta, aiming at solving the problem of the low content of hematopoietic stem cells in the existing hematopoietic stem cell extraction method and the cumbersome operation of the extraction process. , and there are problems such as complex operation and low survival rate of stem cells during cryopreservation

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