An extracting method of an alveolar lavage fluid metagenome

Abstract

A safe, simple, convenient, low-pollution and good-repeatability extracting method of alveolar lavage fluid metagenome DNAs is provided. The method includes steps of liquefying a sample, crushing cells, isolating target DNAs, removing impurity proteins and salt ions, performing DNA adsorption and performing DNA elution. The method can increase the yield and purity of the metagenome DNAs and can meet PCR, DNA library construction, gene sequencing, and other molecular biological experiments.

Description

technical field [0001] The invention provides a method for extracting the metagenome of alveolar lavage fluid, which belongs to the field of biotechnology. Background technique [0002] Lower respiratory tract infection is a relatively common clinical infection, with many types of pathogenic bacteria and strong drug resistance. At present, sputum culture is commonly used for pathogenic diagnosis, but because of its easy contamination, the quality of liquid sputum is difficult to guarantee, which seriously affects the accuracy of diagnosis. Compared with liquid sputum, alveolar lavage fluid has more opportunities to obtain pathogenic bacteria because it is directly collected from the lesion, and can greatly reduce the contamination of samples by oropharyngeal colonization microbiota and improve the isolation rate of pathogenic bacteria. The diagnosis of respiratory infection has higher specificity and sensitivity. The use of alveolar lavage fluid for pathogen diagnosis can ...

AI Technical Summary

Problems solved by technology

At present, sputum culture is commonly used for pathogenic diagnosis, but because of its easy contamination, the quality of liquid sputum is difficult to guarantee, which seriously affects the accuracy of diagnosis

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