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Exosome preserving fluid and exosome preserving method

A preservation method and technology of exosomes, applied in the biological field, can solve the problems of inability to freeze and thaw, affect the in-depth study of exosomes, and require high storage conditions, so as to lower the freezing point, inhibit biochemical reactions and the activities of most enzymes, protect The effect of stability

Active Publication Date: 2020-06-05
中国科学院大学深圳医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term storage requires a low temperature of -80°C, and cannot be frozen and thawed, and requires high storage conditions. These shortcomings affect the in-depth study of exosomes
[0004] Therefore, an exosome preservation solution that effectively maintains the integrity, biological activity and stability of the contents of exosomes remains to be developed.

Method used

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  • Exosome preserving fluid and exosome preserving method
  • Exosome preserving fluid and exosome preserving method
  • Exosome preserving fluid and exosome preserving method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This embodiment is an exosome preservation solution of the present invention, which includes 1 g / L bovine serum albumin, 30 g / L trehalose, 50% (v / v) glycerol, 5% (v / v) dimethyl sulfoxide and 0.01mol / L phosphate buffer; the pH value of the exosome preservation solution described in this example is 7.2.

Embodiment 2

[0055] This embodiment is an exosome preservation solution of the present invention, which includes 10g / L bovine serum albumin, 10g / L trehalose, 30% (v / v) glycerol, 10% (v / v) dimethyl sulfoxide and 0.02mol / L phosphate buffer; the pH value of the exosome preservation solution described in this example is 7.0.

Embodiment 3

[0057] This embodiment is an exosome preservation solution of the present invention, which includes 5g / L bovine serum albumin, 40g / L trehalose, 40% (v / v) glycerol, 8% (v / v) dimethyl sulfoxide and 0.015mol / L phosphate buffer; the pH value of the exosome preservation solution described in this example is 7.5.

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Abstract

The invention relates to an exosome preserving fluid and an exosome preserving method, which belong to the technical field of biology. The exosome preserving fluid comprises bovine serum albumin, trehalose, glycerol, dimethyl sulfoxide and a phosphate buffer solution. The invention also provides the exosome preserving method comprises the following steps: heating the preserving fluid at 85-95 DEGC for 10-30 minutes to form a colloidal solution, and preserving the exosome in a colloidal solution. The exosome preserving fluid provided by the invention can maintain the integrity, biological activity and inclusion stability of exosomes for a long time.

Description

technical field [0001] The invention relates to an exosome preservation solution and a method for preserving exosomes, belonging to the field of biotechnology. Background technique [0002] Exosomes are small vesicles secreted by living cells with a diameter of 30-150nm and a double-layer lipid membrane structure. They are widely found in the body, such as peripheral blood, ascites, urine, saliva, Cerebrospinal fluid and other body fluids are rich in biologically active components such as proteins, lipids, and nucleic acids. Exosomes are involved in material transport, and also carry and transmit important biological information, and play an important role in mediating immune antigen presentation, activation of dendritic cells, occurrence of neurodegenerative diseases, transfer of tumor cell antigens, and tumor diagnosis and treatment , has attracted the attention of many researchers. Before in-depth study of exosomes, the extraction, purification, identification and reason...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 郑义胡波梁欣杨菊红林列坤曾君王长山张慧
Owner 中国科学院大学深圳医院
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