60results about How to "High protein concentration" patented technology

A process for producing ethanol including a combination of biochemical and synthetic conversions results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from carbohydrates, such as corn, using enzymatic milling and fermentation steps, followed by conversion of the acetic acid into ethanol using esterification and hydrogenation reactions. Coproducts can include corn oil, and high protein animal feed containing the biomass produced in the fermentation.

In the recovery of canola protein isolates from canola oil seeds steps are taken to inhibit the formation of colouring components and to reduce the presence of materials tending to form colouring components, to obtain a lighter and less yellow canola protein isolate.

The invention provides a washing method for affinity chromatography in which a wash solution comprising arginine, or an arginine derivative, at pH greater than 8.0, is effective in removing impurities without the presence of a nonbuffering salt, while simultaneously increasing product concentration in the eluate and maintaining a high percent yield of recovered product.

A composition is provided that provides a nutritionally complete calorically dense formula suitable for use as a ready-to-use liquid composition that does not require reconstitution and admixing, which contains intact whey protein in high concentration and which is shelf stable for up to 6 months or more at ambient temperature.

A method for preparing a modified whey protein concentrate (WPC) or whey protein isolate (WPI) is described. It involves (a) providing an aqueous WPC or WPI solution having a protein concentration of 15-50% (w / v), at a pH of 4.7-8.5; (b) heat treating the solution to more than 50° C., for a time that allows protein denaturation to occur; the heat treating comprising heating the solution while under conditions of turbulent flow. At the end of the heat treatment, the heat treated material may be promptly transferred to a drier or to be mixed with other ingredients. The heat-treated WPC or WPI is not subjected to a mechanical shear process prior to the transfer other than where liquid is converted into droplets to facilitate drying. The modified WPC is useful in the manufacture of food and drinks where a high protein content is desired without undesirable changes in texture.

A method is disclosed for producing an infant formula base, wherein by means of microfiltration, ultrafiltration, and nanofiltration the components of milk are separated into a casein fraction, a whey protein fraction, and a lactose fraction to produce, by suitably combining, a composition in which the amino acid composition is close to that of human milk. An infant formula base having a total protein concentration of about 1.0 to about 1.5% and a beta-casein concentration of at least about 11 % of the total protein concentration is also disclosed. The infant formula base is suitable for the production of an infant formula.

A novel approach is described for reversing aggregation and increasing refolding by application of hydrostatic pressure. A protein of interest in an aggregated, or inclusion body, or other non-native or inactive state is subjected to high hydrostatic pressure. This treatment denatures the protein to states (or conformations) competant for refolding and results in increased formation of native protein once pressure is released. The technique can facilitate conversion non-native proteins, including inclusion bodies and aggregates to native proteins without addition of chaotropic agents, changes in buffer, or large-scale dilution of reagents required for traditional refolding methods.

The present invention presents an alternative to the direct implementation of an industrial scale second generation bioethanol process with the integration of the second generation into the existing first generation bioethanol processes, which aims to reduce the current barriers to process change / investments. In particular, the present invention relates to an integrated second generation process for producing bioethanol comprising at least one fungal cultivation stage for producing ethanol and fungal biomass. The present invention also relates to a novel fungal biomass, rich in protein and essential amino acids, which is produced with said integrated second generation process for producing bioethanol for use as a nutritious substitute for human and domestic animal use.

The invention discloses a daily feed for laying hens producing eggs enriched with omega-3 polyunsaturated fatty acid. The daily feed for the laying hens producing eggs enriched with omega-3 polyunsaturated fatty acid is mainly prepared from the following raw materials in parts by weight: 10-20 parts of barley, 5-10 parts of fermented soybean meal, 2-4 parts of beer yeast, 5-10 parts of puffed soybeans, 2-5 parts of puffed linseeds, 3-6 parts of oat bran, 3-6 parts of alfalfa, 2-5 parts of ultrafine wall-broken grape seeds, 2-8 parts of seaweed powder, 0.5 part of a compound premix, 0.003-0.005 part of selenium yeast, 0.05-0.1 part of a compound enzyme preparation, 0.03-0.08 part of betaine, 0.05-0.1 part of probiotics, 0.3 part of Boshan X-1, 0.01-0.03 part of phytase, 0.03-0.1 part of lipase, vitamins, amino acids, and so on. Being used for breeding laying hens, the daily feed is capable of improving immunity and disease resistance of the laying hens, as well as increasing content of omega-3 polyunsaturated fatty acid, especially content of DHA, in the eggs.

This invention is directed to an acid beverage composition, comprising; (A) a hydrated protein stabilizing agent; (B) at least one flavoring material comprising a fruit juice, a vegetable juice, citric acid, malic acid, tartaric acid, lactic acid, ascorbic acid, glucono delta lactone or phosphoric acid; and (C) a slurry of an aqueous protein material wherein the slurry of the aqueous protein material is prepared by a process, comprising; (1) preparing an aqueous extract from a protein containing material, (2) adjusting the pH of the aqueous extract to a value of from about 4 to about 5 to precipitate the protein material, (3) separating the precipitated protein material and forming a suspension of the precipitated protein material in water, (4) adjusting the pH of the suspension to a value of from about 4.0 to about 6.0 to form a slurry of an aqueous protein material, and optionally (5) pasteurizing the slurry of the aqueous protein material; wherein the acid beverage composition has a pH of from 3.0 to 4.5. Also disclosed are several processes for preparing an acid beverage composition.

Disclosed are freeze-dried plasma formats specifically designed for the trauma care field. Blood plasma is subjected to a glucose removal step, a protein fraction up-concentration step and addition of stabilizers prior to freeze-drying. Preferable stabilizers are glutamine dipeptides, glutamine and glycine. The glutamine based formulation is added direct to plasma and serves three main purposes: 1) Increases stability of plasma proteins and stabilizes pH in freeze-dried state; 2) Increases stability of plasma proteins against Gamma Irradiation and thus allows for the application of a terminal sterilization step; 3) Introduces supplements beneficial to the trauma patient.

The invention relates to an exosome preserving fluid and an exosome preserving method, which belong to the technical field of biology. The exosome preserving fluid comprises bovine serum albumin, trehalose, glycerol, dimethyl sulfoxide and a phosphate buffer solution. The invention also provides the exosome preserving method comprises the following steps: heating the preserving fluid at 85-95 DEGC for 10-30 minutes to form a colloidal solution, and preserving the exosome in a colloidal solution. The exosome preserving fluid provided by the invention can maintain the integrity, biological activity and inclusion stability of exosomes for a long time.

The invention discloses a recombinant human fibronectin III1-C (rhFNIII1-C), and a preparation method and application thereof. The preparation method comprises the following steps: optimizing a targetgene segment of the target protein according to codon expression preference, and inserting the obtained gene segment into pET-32a to construct a recombinant plasmid; transforming and introducing therecombinant plasmid into an expression vector BL21(DE3), and carrying out screening to obtain positive clone bacteria capable of realizing efficient soluble expression; carrying out enlarging fermentation culture on the positive clone bacteria, carrying out induced expression, performing crushing and centrifuging to obtain a supernatant, and carrying out purification through elution with a His-tagaffinity chromatography column, dialysis, filtration and other steps to obtain a high-quality rhFNIII 1-C solution with a protein concentration of 1.0 mg / mL or above and a purity of 90% or above. According to detection and observation results of cell adhesion promoting tests, the rhFNIII1-C has the performance of obviously promoting adherence and adhesion of MDBK cells and Balb / c / 3T3 cells and rapid division and growth of the cells, which indicates that the rhFNIII1-C has huge potential of being used as a raw material and a finished product for cosmeceuticals and medical skincare.

This invention provides a high-protein baked food product, comprising at least: at least 15% by weight (on a dry weight basis), based on the whole food product, of a protein component; an oil and fat component and a saccharide component, wherein the high-protein baked food product is obtainable by finely grinding a first material mixture comprising a protein component, an oil and fat component, and a saccharide component, stirring the mixture to prepare a creamy primary product, mixing the primary product with a secondary material mixture provided separately from the primary product and comprising at least an oil and fat component and a saccharide component, and baking the mixture. This baked food product contains protein in a high concentration, is soft and has good chewability and dissolvability within the mouth.

Stable formulations are provided that contain immunoglobulin single variable domains at a high concentration. The formulations are useful as pharmaceutical formulation and suitable for subcutaneous administration. The formulations can be transported and stored under various stress conditions. The invention further relates to containers and pharmaceutical units comprising such formulations and to methods for preparing and prophylactic and therapeutic uses of the formulations and pharmaceutical units of the invention.

The invention belongs to the field of agricultural biotechnology, in particular to a recombinant expression vector for rapidly increasing cellulase activity of Trichoderma reesei and application thereof. The invention uses positive feedback control mechanism to place xyr1 gene under promoter cbh1, pdc1, constructs expression plasmid vector and transforms Trichoderma reesei. Overexpression of xyr1activated both cellulase and self-expression, forming a positive feedback regulatory network, and the cellulase and hemicellulase activities of the positive transformants were significantly increased.

The invention provides a method for removing viruses in preparation of antitoxin and antiserum. The method comprises the following steps of: diluting immunological blood plasma and adjusting pH value; then digesting by stomach enzyme; heating to denaturize; adsorbing by alum; concentrating / desalinating by ultrafiltration; performing ion exchange chromatography to obtain collected liquid; removing bacteria and filtering; and then subpackaging to obtain an antitoxin and antiserum product. Thus, the viruses which probably exist in raw material serum can be removed simultaneously in the process of preparing the antitoxin and antiserum. The virus removal method for the antitoxin and antiserum provided by the invention has the characteristics of simplicity, quickness economy and practicality; the viruses which probably exist in the raw material serum can be removed completely during production to guarantee the quality of the product.

Chimeric anti-microbial proteins, compositions, and methods for the therapeutic and prophylactic treatment of plant diseases caused by the bacterial pathogen Xylella fastidiosa are provided. The anti-microbial proteins of the invention generally comprise a surface recognition domain polypeptide, capable of binding to a bacterial membrane component, fused to a bacterial lysis domain polypeptide, capable of affecting lysis or rupture of the bacterial membrane, typically via a fused polypeptide linker. In particular, methods and compositions for the treatment or prevention of Pierce's disease of grapevines are provided. Methods for the generation of transgenic Vitus vinefera plants expressing xylem-secreted anti-microbial chimeras are also provided.

The invention belongs to the technical field of feed compositions, and specifically relates to a laying hen compound premixed feed for prolonging the shelf lives of eggs and a preparation method thereof, and long shelf life high-selenium eggs. The compound premixed feed is mainly prepared from the following raw materials in parts by weight: 20-30 parts of oat husk powder, 10-15 parts of bio-fermented feed, 11.6-15 parts of expanded linseed, 1.5-3 parts of yeast cell walls, 0.35-0.5 parts of yeast selenium, 0.3 part of vitamin E, 0.4-0.8 part of tea polyphenols, 0.8-1.2 parts of soy isoflavones, and the like. When the premix is used for breeding laying hens, the selenium content of the obtained eggs is more than 45.5 mug / 100 g eggs, and the shelf life at 4-8 DEG C can reach 80 days.

Disclosed are substantially stable sterilized liquid protein supplements including a highly soluble protein source for use with human milk and other infant or adult feeding formulas. The sterilized liquid protein supplements have a more neutral pH, thereby inhibiting protein denaturation and reducing microbial growth. In one aspect, the sterilized liquid protein supplement comprises at least 250 mg of protein per 1 ml, the protein has a degree of hydrolysis of 25% to 45%, a molecular weight median of less than 800 Daltons, and a dipeptide and tripeptide concentration of no more than 40% by weight total protein, wherein the sterilized liquid protein supplement has a pH of 3 to 8.

The present invention belongs to the field of animal feed, particularly to a cattle feed based on bamboo shell. The cattle feed based on bamboo shell comprises the following components in percentage by mass: 26-28% of leymus chinensis, 25-27% of corn, 5-7% of soybean meal, 2.5-3.5% of wheat bran, 4.5-5.5% of rapeseed meal, 0.4-0.6% of salt, 0.4-0.6% of calcium hydrogen phosphate and 30-35% of bamboo shell silage. The total mass percentage of the components is 100%. In the formulation, the leymus chinensis is adopted to increase ingestation amount. If fresh grass is used alone, the growth of animals will be affected. The feed effectively utilizes the waste bamboo shell widely existed. Feeding cattle with the feed is conducive to the synthesis of sugars and carbohydrates, increases the microbial protein concentration in rumen, and increases the utilization rate of protein.

It is described a process to obtain increased milk production and / or greater milk protein concentration using supplements with a fatty acid mixture or through production of specific fatty acids within the animal. The process or the supplementation of the product described in this invention (supplements formulated with fatty acids mixtures and with adequate levels of metabolizable protein) allows greater efficiency and / or better quality and / or a healthier mammal with improved productivity. The process includes supplementation of specific types of fatty acids with or without ruminal protection to ruminants and, generally, a diet with metabolizable protein energy ratio greater than the one suggested by the state of the art. The process may include the production of the said compounds (conjugated fatty acids) on the animal itself from fat, certain compounds and microorganisms added to the diet capable of modifying the environment of the gut. The supplementation includes offering fatty acids capable of altering animal metabolism, as well as offering normal fatty acids (which do not change metabolism which, given some conditions in the rumen environment, can change their molecular structure and transform themselves in fatty acids capable of affecting tissue metabolism (including increasing protein content and yield). Both, the offering of the fatty acid with metabolic effects, as well as the production of these active fatty acids in the gut, make possible increases in the concentration and / or production of milk protein.

The invention relates to artemisia sieversiana essential oil microcapsules and a preparation method thereof, in particular to microcapsules prepared by taking natural plant extract purslane polysaccharide and soy isolate protein as wall materials and artemisia sieversiana essential oil as a core material. Therefore, the stability of the artemisia sieversiana essential oil in humid environments such as light, oxygen, high temperature and humidity can be improved, the activity of the artemisia sieversiana essential oil can be better preserved, and the release speed and uniformity of the artemisia sieversiana essential oil can be controlled. As the wall material is the natural plant extract, the safety is good, and the wall material also has rich nutritional value and medicinal value. The purslane polysaccharide and the soy isolate protein are used as wall materials together, so that the medicinal value of the purslane polysaccharide can be applied, the embedding rate of the microcapsule can be increased, and stable product quality is obtained.