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Sperm diluent preparing method

A configuration method and diluent technology, applied in the field of medical testing, can solve problems such as sperm movement obstruction, semen not liquefied, semen not liquefied, etc.

Inactive Publication Date: 2015-06-03
TIANJIN BAODI HOSPITAL
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

3. Lack of trace elements: male lack of trace elements such as magnesium, zinc, etc., can also lead to symptoms of non-liquefied semen
The movement of the sperm in this kind of semen in the female reproductive tract is obviously hindered. The sperm cannot go up into the cervical canal, uterine cavity and fallopian tube, and cannot meet the egg, which makes fertilization difficult. The semen diluent formula recommended in the National Clinical Laboratory Operation Regulations, because the semen is not liquefied, the sperm motility, sperm motility rate, sperm morphology and quantity cannot be checked, so there is an urgent need for a method that can quickly improve the semen liquefaction time and change the semen liquefaction. , a sperm diluent that does not change the motility and state of sperm

Method used

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Experimental program
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Embodiment 1

[0008] 1. Source of specimens 84 male infertility outpatients in our hospital, with an average age of 32 years old, took their semen by masturbation and placed them in containers.

[0009] 2. The instrument WLJY-9000 Weili color sperm quality detection system is provided by Beijing Weili New Century Technology Development Co., Ltd.

[0010] 3. Methods According to the degree of semen liquefaction, the patients were divided into liquefaction group and non-liquefaction group. Natural liquefaction in 37°C warm bath and 37°C warm bath with the same amount of sperm diluent mixed with semen were carried out respectively. The liquefaction time was observed and sperm motility was detected.

[0011] 4. Measurement results

[0012] 4.1 Routine comparison of semen in the liquefaction group is shown in Table 1

[0013] Table 1 Comparison of sperm movement speed (μm / s) with different sperm treatment solutions

[0014]

[0015] When measuring the sperm motility rate, there was no signi...

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Abstract

The invention discloses a sperm diluent preparing method and belongs to the technical field of medical examination. A diluent prepared with the method contains substances such as glucose, citric acid, sodium citrate, yolk, glycerol, N-acetyl-L-cysteine, penicillin and the like. The diluent does not affect motility and quality of sperms and can shorten the semen liquidation time and the patient waiting time, detection of indexes such as the number, the motility, the malformation condition and the like of the sperms can also be performed on a patient whose semen is not liquidized, and the motility of the sperms can be kept for a long time. The detection method which is simple in pretreatment, quick, sensitive, cheap and capable of realizing long-acting preservation is applicable to conventional detection of the semen.

Description

technical field [0001] The invention discloses a sperm diluent preparation method, which belongs to the technical field of medical examination. Background technique [0002] Semen is not liquefied, which means that the semen is still jelly-like for more than 30 minutes after it is excreted. The common cause of semen not liquefied is the lack of fibrinolytic enzyme secreted by the prostate caused by seminal vesiculitis and prostatitis, and the reasons for the non-liquefied semen and pathogenic microorganism infection Related: 1. Prostatitis: The prostate is involved in the process of coagulation and liquefaction of semen, and semen liquefaction factors such as proteolytic enzymes and fibrinolytic enzymes produced by the prostate make the semen liquefy. Once the prostate is inflamed, the secretion of the above factors can be hindered, the liquefaction factors are reduced, and semen is not liquefied. 2. Seminal vesiculitis: the secretion of the seminal vesicles participates in...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 常玉芝丁弘李立和
Owner TIANJIN BAODI HOSPITAL
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