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Industrial fermentation method of phytase

A fermentation method and phytase technology, which are applied in the industrial fermentation field of phytase, can solve the problems of outdated fermentation process, difficulty in degrading phytate, and reduced digestibility, etc., so as to facilitate absorption, promote metabolic enzyme production, and maintain penetration. pressure effect

Inactive Publication Date: 2018-01-16
山东奥博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the phosphate bond in phytic acid is very stable. There are generally two methods for the degradation of phytic acid. One is chemical methods, but it is extremely difficult to degrade phytic acid by chemical methods; the other method is Enzymatic hydrolysis, the currently known enzyme that can effectively degrade phytic acid is only phytase
[0004] Monogastric animals lack phytase to decompose phytic acid. Phosphorus in the form of phytic acid is difficult to be absorbed and utilized by animals, which brings the following problems: First, the presence of phytic acid in feed has anti-nutritional effects. Phytic acid Phosphorus itself is difficult to be hydrolyzed and utilized by the digestive tract of pigs, poultry and aquatic animals; phytic acid will combine with various metal ions and proteins during the digestion process of animals' gastrointestinal tract to form insoluble complexes, which will hinder the action of some digestive enzymes. Inhibition, which greatly reduces the digestibility of mineral elements and other nutrients in the feed
The traditional industrial fermentation of phytase often adopts fermentation, fed-batch fermentation, methanol induction, and there are problems such as outdated fermentation process, high cost and low benefit

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1. An industrial fermentation method of phytase is characterized in that the method comprises the following steps.

[0044] Step 1: Primary fermenter fermentation

[0045] Put primary fermentation medium and polyether modified silicon defoamer in the primary fermentation tank, the volume ratio of fermentation medium and polyether modified silicon defoamer is 6000-8500:1; heat the fermentation medium , raise the temperature of the fermentation medium to 121-123°C, keep the tank pressure at 1.1-1.4MPa, and sterilize for 25-40min; At -35°C, transplant the fermented seed liquid into a first-stage fermenter with an inoculum volume ratio of 5-10% to expand the culture, add trace element solution to the fermenter, and adjust the pH of the fermented liquid with ammonia water 4.8-5.0, the volume ratio of trace element solution and fermentation medium is 1:20-25; the inoculum volume of fermented seed liquid is 1.5-2%; the rotation speed of the primary fermentation tank ...

Embodiment 2

[0070] Example 2. An industrial fermentation method of phytase is characterized in that the method comprises the following steps.

[0071] Step 1: Primary fermenter fermentation

[0072] Put primary fermentation medium and polyether modified silicon defoamer in the primary fermentation tank, the volume ratio of fermentation medium and polyether modified silicon defoamer is 6000-8500:1; heat the fermentation medium , raise the temperature of the fermentation medium to 121-123°C, keep the tank pressure at 1.1-1.4MPa, and sterilize for 25-40min; At -35°C, transplant the fermented seed liquid into a first-stage fermenter with an inoculum volume ratio of 5-10% to expand the culture, add trace element solution to the fermenter, and adjust the pH of the fermented liquid with ammonia water 4.8-5.0, the volume ratio of trace element solution and fermentation medium is 1:20-25; the inoculum volume of fermented seed liquid is 1.5-2%; the rotation speed of the primary fermentation tank ...

Embodiment 3

[0099] Example 3. An industrial fermentation method of phytase is characterized in that the method comprises the following steps.

[0100] Step 1: Primary fermenter fermentation

[0101] Put primary fermentation medium and polyether modified silicon defoamer in the primary fermentation tank, the volume ratio of fermentation medium and polyether modified silicon defoamer is 6000-8500:1; heat the fermentation medium , raise the temperature of the fermentation medium to 121-123°C, keep the tank pressure at 1.1-1.4MPa, and sterilize for 25-40min; At -35°C, transplant the fermented seed liquid into a first-stage fermenter with an inoculum volume ratio of 5-10% to expand the culture, add trace element solution to the fermenter, and adjust the pH of the fermented liquid with ammonia water 4.8-5.0, the volume ratio of trace element solution and fermentation medium is 1:20-25; the inoculum volume of fermented seed liquid is 1.5-2%; the rotation speed of the primary fermentation tank ...

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Abstract

The invention discloses an industrial fermentation method of phytase. The method comprises the following steps: (1) fermentation in a first-level fermentation tank; (2) fermentation in a second-levelfermentation tank; (3) high-density fermentation; (4) fed-batch fermentation and starving; (5) induction and feed mixing. The industrial fermentation method of phytase disclosed by the invention has the characteristics of high tank enzyme activity and high fermentation efficiency.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to an industrial fermentation method of phytase. Background technique [0002] Phytic acid (Phytate, Phytic, IP6), also known as phytate, contains 6 phosphate groups and is an important storage form of phosphorus in feed. The molecular formula of phytic acid is C 6 h 18 o 24 P 6 , the molecular weight is 660.09. Phytase (Phytase) can catalyze the hydrolysis of phytic acid and phytate into inositol and phosphoric acid (or phosphate). [0003] Phytic acid mainly exists in plant seeds in the form of calcium, magnesium, and potassium phytate, and also exists in nucleated red blood cells of animals. Phytase is found in enzymes secreted by soil, plants, and microorganisms to the outside of the cell. Therefore, phytase plays an important role in the metabolic cycle of phosphorus in the natural environment. In addition, phytic acid has a strong complexing ability for most meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12R1/84
Inventor 付振山赵士辉黄兴义王淑华
Owner 山东奥博生物科技有限公司
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