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Preparation method of remolded compound biological amniotic membrane

An amniotic membrane and biological technology, applied in medical science, prosthesis, etc., can solve the problems that the biological function of biological amniotic membrane cannot meet clinical requirements, damage collagen, collagen fibers, natural collagen and active factors are easily oxidized, etc. And protect the active tissue of the human body, stabilize the cell membrane, and have a significant effect of anti-aging.

Inactive Publication Date: 2019-01-08
谭亚
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Problems solved by technology

[0013] The technical problem mainly solved by the present invention is that a large amount of natural collagen and active factors in the amniotic membrane are easily oxidized during the process of repeated freeze-thawing, drying and decellularization of the current biological amniotic membrane, and organic chemical reagents will destroy collagen and collagen fibers. and other components, resulting in the defect that the biological function of the bio-amnion cannot meet the clinical requirements, and a preparation method for remodeling the composite bio-amnion is provided

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  • Preparation method of remolded compound biological amniotic membrane

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Embodiment Construction

[0029] Take the placenta and place it on a sterile workbench, bluntly separate the amniotic membrane and chorion from the placenta, wash the blood stains with normal saline to obtain clean amniotic membrane, and then use 4 to 5×10 6 After washing with U / L gentamycin, put it into DMEM medium and culture it for 4-5 days, wherein DMEM medium contains 100~120×10 3U / L penicillin and streptomycin, as well as calf serum with a volume fraction of 10%, were frozen and stored at -25 to -20°C after the culture to obtain pretreated amniotic membranes for use; Add water to yeast to prepare yeast milk with a mass fraction of 15%, adjust the pH of the yeast milk to 4.5-5.0 with a 20% sodium citrate solution, heat to 50-55°C, add 0.5% yeast milk and mix Protease, wherein the mixed protease is formed by mixing papain and bacterial protease at a mass ratio of 2:1, enzymatically hydrolyzing for 4-6 hours to obtain an enzymatic hydrolyzate; 25% of linoleic acid is mixed and placed in a high-spee...

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Abstract

The invention relates to the technical field of medical material preparation, in particular to a preparation method of a remolded compound biological amniotic membrane. Protease is prepared from active dry yeast as a raw material, enzymatic hydrolysate and linoleic acid are sheared, meanwhile, ascorbic acid is added, and a biopeptide oxidant is obtained; procyanidine has quite strong in-vivo activity, can protect human cellular tissue from oxidative damage caused by free radicals, and has the biological characteristics of strengthening and protecting living tissue of a human body, stabilizingcell membranes and resisting enzymatic activity. With the combination of guar gum and an amniotic membrane, mercapto groups of active proteins in the amniotic membrane are protected by physical barrier of the guar gum, oxidization of active groups is reduced, insoluble amniotic membrane collagen can suspend for a long time on the basis of rheological property of the guar gum, the guar gum is beneficial to low-temperature pulverizing and normal-temperature preservation of the amniotic membrane, and the preparation method has broad application prospect.

Description

technical field [0001] The invention relates to the technical field of medical material preparation, in particular to a preparation method for remodeling composite biological amniotic membrane. Background technique [0002] Tissue adhesions are abnormal structures formed by the combination of connective tissue fibrous bands and adjacent tissues or organs, which can cause various postoperative complications: for example, adhesions after tendon repair, seriously affecting the recovery of the function of the surgical site, and even Can cause loss of local function. [0003] The amniotic membrane refers to the basement membrane layer of the human placenta, which is also the innermost layer of the placenta. It is smooth and elastic, with a thickness of about 0.02 to 0.5 mm. Amniotic membrane is a collagen tissue layer without blood vessels and cell structure, rich in IV and V collagen, basic fibroblast growth factor, stem cell growth factor, easy to make epithelial cells migrate...

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Application Information

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IPC IPC(8): A61L27/36
CPCA61L27/3604A61L27/3687A61L27/3691
Inventor 谭亚章红英赵金晶
Owner 谭亚
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