124 results about "Cell fixation" patented technology

Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is used to control the intensity and duration of the voltage to assure that electroporation has occurred without destroying the cell(s). This is applicable to electroporation in general. In addition, a particular method and apparatus are disclosed in which electroporation and / or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. The barrier is either electrically insulating, impermeable to the solute, or both, depending on whether pore formation, diffusive transport of the solute across the membrane, or both are sought. Electroporation is achieved by applying a voltage between the two chambers, and diffusive transport is achieved either by a difference in solute concentration between the liquids surrounding the cell and the cell interior or by a differential in concentration between the two chambers themselves. Electric current and diffusive transport are restricted to a flow path that passes through the opening.

Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby allowing more accurate analysis and classification of circulating tumor cells (CTC) and, ultimately, of tumor burdens in cancer patients. Stabilization of specimens is particularly desirable in protocols requiring rare cell enrichment from blood specimens drawn from cancer patients. Exposure of such specimens to potentially stressful conditions encountered, for example, in normal processing, mixing, shaking, delays due to transporting the blood, has been observed to not only diminish the number of CTC but also to generate debris and aggregates in the blood specimens that were found to interfere with accurate enumeration of target cells, if present. Stabilizers are necessary to discriminate between in vivo CTC disintegration and in vitro sample degredation.

A chemical sensor utilizing a chemical receptor (for example, one stimulating the sense of taste or smell) is provided. More specifically speaking, such a receptor is introduced into cells and the cells are immobilized on a support to form a chip. This chip is then employed as a component of a sensor. This sensor shows a reaction almost the same as the body's perception of the taste or smell or sense, thereby enabling analysis. Thus, it is also usable as an artificial sensory organ. Moreover, this sensor is usable in diagnosis, which imparts a high industrial usefulness to it.

The cell selection apparatus includes: a cell selection vessel which has a pair of electrodes and a sheet-like insulating material having a plurality of micropores, with a recognition molecule bindable to the specific substance being disposed on a bottom face of the micropore; and a power supply, wherein the power supply includes a cell-immobilization power supply and a cell-taking power supply. The cell selection method uses the cell selection apparatus, including; introducing cells into a cell selection area; immobilizing these cells in the micropores; effecting a binding reaction between the specific substance and the recognition molecule; thereafter, taking out a cell of which the specific substance is not or is weakly bound to the recognition molecule, from the micropore; otherwise alternatively, leaving a cell of which the specific substance on the surface of the cell is strongly bound to the recognition molecule, behind in the micropore.

The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

The invention provides a kit and method for inducing differentiation from bone mesenchymal stem cells to osteoblasts. The kit comprises an osteoblast inducing culture solution and an osteoblast identification solution, wherein the inducing culture solution comprises a cell culture solution A, a cell culture solution B, a cell culture solution C, a cell culture solution D and a cell culture solution E; the osteoblast identification solution comprises a cell immobilizing solution, coloring agents and a detergent; the cell culture solution A is an alpha-minimum essential medium (MEM) liquid culture medium; the cell culture solution B is fetal bovine serum; the cell culture solution C is a dexamethasone solution; the cell culture solution D is a beta-sodium glycerophosphate solution; the cell culture solution E is a vitamin C solution; the cell immobilizing solution is 4% paraformaldehyde; the coloring agents include sodium thiosulfate, silver nitrate and neutral red; and the detergent is double distilled water.

The invention relates to a cervical exfoliated cell preservation solution with a pretreatment function and application, and in particular to a cervical exfoliated cell preservation solution which contains cell fixative, anticoagulant, PH buffer, humectant, osmotic pressure-maintaining agent, microbe inactivator, mucus digestion ingredient and red blood cell treatment ingredient. The preservation solution can effectively preserve cervical exfoliated cells, protect the integrity and fixity of cells and prevent the pycnosis, cytolysis and distending of cells; the mucus treatment capability and the blood cell disintegration capability are high; the cell preservation time is up to 30 days; the slide preparation effect is flat and even, and preserved cells can also be applied in the preparationof cell wax blocks, HPV virus detection and the like; the effective period of the preservation solution is two years under normal temperature, the microbe killing capability is high, and the health ofmedical technicians is effectively protected.

The invention belongs to the field of immunodetection and discloses a method for the quantitative determination of specific cytotoxicityT lymphocyte of hepatitis B virus. The method adopts the technical proposal that the major histocompatibility compound of antigen peptide pentamer, a mouse anti-human CD3 monoclonal antibody and a mouse anti-human CD8 monoclonal antibody which are labeled by different fluoresceins are incubated with the anticoagulant peripheral blood of an HLA-A0201 or HLA-A2402 positive masculine hepatitis B virus carrier for a proper period; and after the operations of hematid schizolysis, centrifugal washing and cell fixation are completed, the CD3 gating technique is used for the quantitative determination of the specific cytotoxicityT lymphocyte of hepatitis B virus on a flow cytometry. The invention can increase the determination of the specificity, the sensitivity and the stability of the specific CTL.

The invention provides a liquid-based thin-layer cell preserving fluid. The preserving fluid comprises the following components in percentage by mass: 5-6.5% of a pH buffer solution, 0.3-0.5% of a chelating agent, 0.2-0.8% of an osmotic pressure maintaining agent, 10-26% of a cell fixing agent, 0.1-5% of a preservative, 0.5-10% of a hemolytic agent, 1-4% of a water-soluble adhesive and 66-80% of ultrapure water. The liquid-based thin-layer cell preserving fluid can keep the stability of a cell structure well and keep the pH value stable, so that cells are not easy to agglomerate, and pathological examination is facilitated.

The invention relates to a blotting material used for specific recognition of cells, and preparation and applications thereof. According to the blotting material, cells are taken as a template, cell culturing and cell immobilization technology are adopted to immobilize the cells on the surface of a substrate material, curing of a pre-polymerization system is carried out on the surface of the substrate material, the substrate material is removed via separation, and the template cells on the surface of an obtained cured polymer are removed so as to obtain a finished product. The blotting material is high in selectivity and stability, and can be used in identification, capturing, and enriching of cells.

The present invention provides a cell array structural body containing a substrate, a plurality of micropores piercing the substrate from one surface to another surface, through which a sample cell can pass, and a capture / release unit for the sample cell on a wall surface of each micropore, as well as a cell array with such structural body detachably immobilizing sample cells in its micropores. In using the cell array structural body and the cell array for drug evaluations or the like, handling of a culture medium or a drug solution, or washing procedure is easy, and harvest of a desired cell from the cell array is easy and trustworthy.

The invention provides a kit for detecting human regulatory T cell subtypes and a detection method and belongs to the technical field of cell subtype detection. The provided kit comprises components as follows: a blood diluent, a mononuclear cell separation medium, a cell culture fluid, a lymphocyte activation solution, dead cell removal dyes, an FcR blocking agent, a fluorescence labeled antibodyresisting human cell surface labeling molecules, a fluorescence labeled antibody resisting human intracellular molecules, a PBS buffer solution, lipopolysaccharide, a washing buffer solution, a celldyeing buffer solution, a cell fixing solution and a permeable membrane lotion. During detection, firstly, mononuclear cells in whole blood are separated, then, mononuclear cells of human peripheral blood are stimulated, finally, a fluorescent antibody is stained, and the regulatory T cell subtypes can be detected. By use of the kit and the detection method, 2-6 regulatory T cell subtypes can be simply and rapidly distinguished.

The invention provides a cell fixation solution, detection kit and method for human respiratory tract pathogen infection flow cytometry detection. The cell fixation solution is a PBS solution containing formaldehyde with the volume fraction of 0.1-1% and methyl alcohol with the volume fraction of 60-80%, and the pH of the cell fixation solution is 7.2-7.4. The kit comprises the cell fixation solution, a cell permeating agent and at least one kind of monoclonal antibodies of a human respiratory tract pathogen to be detected, each kind of monoclonal antibodies of the human respiratory tract pathogen to be detected is marked with fluorescein, and different human respiratory tract pathogens to be detected are provided with different fluoresceins. The detection process is automatic, labor cost is saved, the result is objective and accurate, single-sample multiple detection can be achieved, and the pathogen infection condition can be better reflected.

The invention discloses a method for preparing the substrates of edible mushrooms of water hyacinth, comprising the following steps: the water hyacinth is air-cured so as to reduce the moisture content of the plant thereof to 8 present to 12 percent, the leafstalk is treated by adopting the method of plant tissue cell fixation, then the treated leafstalk is put into hoggery-pigsty washing liquor or Biogas Slurry to be soaked for 7 to 10 days, and then is taken out, dried and crushed, then the substrate of edible mushrooms which can replace the sawdust and improve the amino acid content is obtained. The invention has the advantages that the invention overcomes the original defective characteristic of the leafstalk of the water hyacinth, and provides a substrate of edible mushroom which can replace the sawdust and improve the amino acid content. While making the waste water hyacinth profitable, the invention reduces the dependence of the production of the edible mushrooms on the sawdust.

The invention particularly relates a toxic product tolerating cell immobilized method and a continuous production process of 1,5-diaminopentane by immobilized cell through transformation. According to the invention, the ratio of polyvinyl alcohol to sodium alginate and a corresponding gel forming method are developed and optimized, the prepared immobilized cell has the advantages that damage to bacterial cells from 1,5-diaminopentane with high concentration is tolerated, the expansion character is better, the influence of release of a large amount of carbon dioxide gas on a structure in the transformation process is tolerated, the mechanical strength is high, and the service life is long; the activity of the prepared immobilized cell can be kept 98 percent or above after being used for 6 times in batches, the activity of the prepared immobilized cell can be kept 70 percent or above after being continuously used for 12 hours. The continuous production process provided by the invention has the advantages that needed devices are less, the operation is convenient, amplification of production scale is easy, and the degree of automation is high; no neutralizer is added in the production process, and the product is easy to clarify, separate and purify.

The invention relates to a non-cervical exfoliative cell preserving solution with a pretreatment function and application thereof, in particular to a non-cervical exfoliated cell preserving solution containing a cell fixative, a cleaning solution, an osmotic pressure maintenance agent, a microbial inactivating agent, and a cell lysis component.Thenon-cervical exfoliative cell preserving solution canbetter preserve non-cervical exfoliated cells comprising mucus samples, body fluid samples, puncture and brush samples to protect cell integrity and fixation. At the same time, various parts of thecells can be easily colored to be suitable for observation, long-term preservation, and analysis, and the preserved cells can also be used for cell wax blockmaking and immunohistochemical detection.

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

The invention discloses a method for fast detecting allergens, belonging to the technical field of detection. The integrated type allergen viable cell detection system integrates cell fixation, allergen irritation and histamine release and determination. The system comprises three cavities consisting of four layers of dimethyl siloxane microchips, wherein the cavities are connected respectively through a silane capillary tube, the cavity at the first layer can be connected with a flow injector or a syringe through a plurality of joints, the cavity at the second layer is an effect cell cavity, and the cavity at the third layer is a histamine reaction basin cavity. After liquid to be detected continuously and fully reacts with effect cells, a solution reaches a histamine reaction basin through a filter membrane and fully reacts with fluorescent derivatization liquid input in advance through reaction liquid injection holes, the generation condition of histamine can be judged under a fluorescent microscope according to fluorescence intensity, and further whether the reaction liquid contains allergens can be judged. The method has excellent sensitivity, fast response and direct visibility, the detection time can be remarkably shortened, the stability is high, and the method has great application potential in detection of allergens in cosmetics, food safety and clinical medicines.

The invention relates to a lattice-type three-dimensional cell culture support and its manufacturing method and use method. The lattice-type three-dimensional cell culture support comprises a quartz optical template with micropores. The quartz optical template with the micropores is made of a quartz optical template with films. The micropores form an inclined column-type micropore array. The manufacturing method comprises the following steps of preparing the quartz optical template with the films, then preparing the inclined column-type micropore array, and removing the film of a polymer material. The use method of the lattice-type three-dimensional cell culture support comprises high temperature disinfection of the lattice-type three-dimensional cell culture support, surface modification, cell implantation and culture, cell fixation, cell permeabilization and enclosing, cell incubation and cell cleaning. The manufacturing method provided by the invention can adjust aperture sizes, distribution and crystal lattice layer number of the lattice-type three-dimensional cell culture support, can effectively improve scientific and complete experimental data for the tissue engineering research, can improve cell culture efficiency and can reduce the frequency of animal experiments. The lattice-type three-dimensional cell culture support is suitable for large-scale production, has a low cost and can satisfy wide requirements of regenerative medicine.

Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby allowing more accurate analysis and classification of circulating tumor cells (CTC) and, ultimately, of tumor burdens in cancer patients. Stabilization of specimens is particularly desirable n protocols requiring rare cells enrichment from blood specimens drawn from cancer patients. Exposure of such specimens to potentially stressful conditions encountered, for example, in normal processing, mixing, shaking, delays due to transporting the blood, has been observed to not only diminish the number of CTC but also to generate debris and aggregates in the blood specimens that were found to interfere with accurate enumeration of target cells, if present. Stabilizers are necessary to discriminate between in vivo CTC disintegration and in vitro sample degredation.

The invention discloses a bionic artificial periosteum with a "sandwich" structure and a preparation method of the bionic artificial periosteum. The bionic artificial periosteum comprises a fiber layer electrospinning membrane and a germinal layer electrospinning membrane, and at least one of the following cell sheet layers is laid between the fiber layer electrospinning membrane and the germinal layer electrospinning membrane to form the sandwich structure: an osteoblast precursor cell sheet layer, a mesenchymal stem cell sheet layer and a vascular endothelial cell sheet layer; at least one cell sheet layer is arranged; the fiber layer electrospinning membrane is a spinning membrane made of a degradable high polymer material and a natural high polymer material; the germinal layer electrospinning membrane is a compound of a natural high polymer material or natural high polymer material and a degradable high polymer material. According to the bionic artificial periosteum, the nanofiber membrane is compounded with the cell sheet layers, so that the strength of the cell sheet layers can be improved, the requirements of surgical operations such as suturing can be met, the cells can be fixed between the nanofiber membranes by utilizing the cell shielding effect of the nanofiber membrane, so that the cells are effectively prevented from diffusion after being implanted into a body, and the cell utilization rate is effectively improved.

An isotonic hemolysin comprises an oxidation dissolving agent, a cosolvent, a cell solubilizer, a cell fixing agent, inorganic salts and water. The oxidation dissolving agent is sodium nitrite, and the content of the oxidation dissolving agent is 2-20g / L. The cosolvent comprises one or more of glycerol, diethylene glycol and propylene glycol, and the content of the cosolvent is 0.3-3mmol / L. The cell solubilizer comprises one or more of isobutanol, methanol and ethanol, and the content of the cell solubilizer is 0.1-1mol / L. The cell fixing agent comprises one or more of paraformaldehyde, formaldehyde, ethanol and acetone, and the content of the cell fixing agent is 10-40g / L. The inorganic salts comprise sodium chloride, magnesium chloride and calcium chloride, wherein the content of the sodium chloride is 1-10g / L, the content of the magnesium chloride is 1-100mmol / L, and the content of the calcium chloride is 1-100mmol / L. A preparation method of the hemolysin, a method for treating a biological sample by the hemolysin and a method for detecting a leukocyte membrane antigen by a flow cytometry are presented. After a sample is treated by the hemolysin, each population of leukocytes ispartitioned obviously, leukocyte membrane protein is not damaged, the original biological activity is retained, and red blood cells can be completely split. The result is accurate, and the performance is stable.

The invention discloses a p53 protein and mitochondria double-labeled immunofluorescence detection method and a kit thereof. The detection method comprises the steps of: preparing a cell climbing piece, and performing cleaning; fixing the cell climbing piece with cell fixing liquid, and performing cleaning; sealing the cell climbing piece with cell confining liquid; adding primary antibodies for incubation, and performing rinsing, wherein the primary antibodies comprise an antibody combined with p53 protein and an antibody combined with mitochondria; adding a fluorescently labeled secondary antibody for dyeing, and performing incubation and cleaning; performing redyeing by using cell nucleus fluorochrome, and performing cleaning; sealing the piece, and observing a dyeing result via microscopic examination of a fluorescence microscope or via the microscopic examination of a confocal microscopy; and performing superposed analysis on fluorescent pictures. Only a cytomembrane is broken, anuclear membrane is not broken, intranuclear signal interference can be avoided, co-localization of p53 protein and mitochondria is realized, and basis and reference are provided for the positioning of the mitochondria of protein and the positioning study of the protein and other non-nuclear organelles.

The invention discloses a single-cell slide preparation method based on liquid-based cell slide preparation. The method comprises the following step of liquid-based sample treatment: putting pretreated liquid-based samples into a treatment vessel one by one, and carrying out 37 DEG C incubation, reaction system switching, red blood cell flushing removal, PBS buffer solution replacement, white blood cell interference removal by adding magnetic beads and the like on the liquid-based samples. In the invention, through technological processes of treating the liquid-based samples, adding a cell preservation treatment solution, precipitating, carrying out papanicolaou staining, and carrying out centrifugal slide preparation, problems that an existing single-cell slide preparation method is poorin slide preparation quality and is easy to lose effective diagnosis cells, smears are different in thickness, missed diagnosis is often generated, a large number of leukocytes and erythrocytes oftenremain in a sample, and observation is affected are solved; and the single-cell slide preparation method based on the liquid-based cell slide preparation is high in cell preservation stability, good in cell fixation, more complete in reserved cell form and capable of removing redundant cells.

A battery module comprises an electrochemical cell including a first dielectric layer positioned on a first side of the cell, a first side plate in opposition to the first side, and a first adhesive layer positioned between the first side plate and the first side. The first dielectric layer defines a first window. The first adhesive layer is adhered to the first side plate and the first side through the first window. Another battery module comprises first and second cells, a base plate, a frame, and a first and second adhesive portion. The frame includes a beam positioned between the first cell and the second cell and is in engagement with a first side and a second side of the first and second cells, respectively. The first adhesive portion and the second adhesive portions are positioned between the base plate and the bottom surfaces of the cells.

The invention discloses a kit for verifying endodontium mesenchymal stem cells. The kit comprises a flow phenotype detection reagent set, cell fixation liquid, an ordinary culture medium, an adipogenesis induction and detection reagent set, an osteogenesis induction and detection reagent set and a chondrogenesis induction and detection reagent set. Usually, conventional osteogenesis induction and adipogenesis induction need about 24 days, consumed time is long, and induction efficiency is low; by the kit, the time for osteogenesis induction and adipogenesis induction is shortened to 18 days, induction efficiency is improved, and induction time is shortened; conventional flow detection mainly aims at universal surface markers of mesenchymal stem cells, but the endodontium mesenchymal stem cells have positively-expressed surface markers CD146 which are different from the surface markers of other mesenchymal stem cells; CD146 antibodies are added into the kit, and accordingly, accurate verification results can be acquired.

The invention discloses a kit for verifying adipose-derived stem cells. The kit comprises a flow phenotype detection reagent set, cell fixation liquid, an ordinary culture medium, an adipogenesis induction and detection reagent set, an osteogenesis induction and detection reagent set and a chondrogenesis induction and detection reagent set. Usually, conventional osteogenesis induction and adipogenesis induction need about 24 days, consumed time is long, and induction efficiency is low; by the kit, the time for osteogenesis induction and adipogenesis induction is shortened to 18 days, induction efficiency is improved, and induction time is shortened; conventional flow detection mainly aims at universal surface markers of mesenchymal stem cells, but the adipose-derived stem cells have positively-expressed surface markers CD49d and negatively-pressed CD106 which are different from the surface markers of other mesenchymal stem cells; CD49d antibodies and CD106 antibodies are added into the kit, and accordingly, accurate verification results can be acquired.

The invention relates to the technical field of biological preserving fluid, in particular to cell preserving fluid for in-vitro analysis and detection and a preparation method thereof. The cell preserving fluid is prepared from the following components in a concentration range: 60 to 75 v / v percent of a cell fixing agent, 0.6 to 0.9 g / 100 mL of an osmotic pressure regulator, 0.2 to 0.5 g / 100 mL of an anticoagulant, 0.3 to 0.6 g / 100 mL of chitosan quaternary ammonium salt and 0.05 to 0.2 g / 100 mL of trehalose; and the solvent is ultra-pure water, and the pH value is 7.0-7.6. A small amount of trehalose and chitosan quaternary ammonium salt are combined, so that the protective effect can be effectively enhanced, autolysis decay of cells is effectively reduced, and the integrity of cell preservation is improved. When the cell preserving fluid is prepared, ultra-pure water is used for dissolving components except the cell curing agent, the dissolving effect of the components can be effectively improved, the uniform and stable cell preserving fluid is obtained, and batch production of the cell preserving fluid is facilitated.