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Single-cell slide preparation method based on liquid-based cell slide preparation

A liquid-based cell and single-cell technology, applied in the field of cytology detection, can solve the problems of poor production quality, different thicknesses of smears, and many residual white blood cells and red blood cells, etc., and achieves high stability, good cell fixation, and retention. The effect of cell morphology integrity

Inactive Publication Date: 2020-04-24
HANGZHOU JUNHUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a liquid-based cell sheeting method for single-cell sheeting, which has the advantages of high cell stability, good cell fixation, and more complete cell morphology, as well as the ability to remove redundant cells. The quality of the film production method is poor, it is easy to lose effective diagnostic cells, the thickness of the smear is different, it is easy to miss the diagnosis, and there are often a lot of white blood cells and red blood cells in the sample, which affects the problem of observation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Liquid-based sample processing: put the pretreated liquid-based samples into processing vessels one by one, incubate the liquid-based samples at 37°C, switch the reaction system, wash red blood cells to remove them, change PBS buffer, add magnetic beads to remove white blood cell interference, etc. Treatment; add cell preservation treatment solution; add the liquid-based specimen to the cell preservation treatment solution and shake fully; sedimentation: pour the treated liquid-based sample into the sedimentation chamber, let it settle, and suck off the supernatant; Papanicolaou staining: liquid The basic sample was left to settle, after absorbing the supernatant, put it into absolute ethanol or isopropanol, into pH 7.8 tris buffer, into hematoxylin staining solution, into pH 7.8 tris buffer, Propanol, put in EA-OG mixed dye solution, put in absolute ethanol or isopropanol, repeat 2 times, put in xylene; centrifuge to make slices, transfer the liquid-based sample after P...

Embodiment 2

[0031] In embodiment one, add following operation:

[0032] In step 1, the pretreatment includes liquid-based sample sorting, placement, and code scanning. The incubation time at 37°C is 8-12 minutes. The reaction system is switched to a normal saline system. For 3-5min, remove red blood cells and prepare for subsequent operations.

[0033] Liquid-based sample processing: put the pretreated liquid-based samples into processing vessels one by one, incubate the liquid-based samples at 37°C, switch the reaction system, wash red blood cells to remove them, change PBS buffer, add magnetic beads to remove white blood cell interference, etc. Treatment; add cell preservation treatment solution; add the liquid-based specimen to the cell preservation treatment solution and shake fully; sedimentation: pour the treated liquid-based sample into the sedimentation chamber, let it settle, and suck off the supernatant; Papanicolaou staining: liquid The basic sample was left to settle, after a...

Embodiment 3

[0035] In embodiment two, add following operation:

[0036] In step 2, the cell preservation treatment solution is composed of the following raw materials in parts by weight: 35 parts of ethanol, 3 parts of sodium citrate, 5 parts of sodium chloride, 7 parts of N-acetylcysteine, 6 parts of dithiothreitol 3 parts of proline, the volume ratio of liquid-based specimens and cell preservation treatment solution is 1:3, which can remove red blood cells in liquid-based specimens and fix the original form of cells with diagnostic value in time; for mucus and epidermal tissue Softening and degradation are performed to separate effective cells from the mucus.

[0037] Liquid-based sample processing: put the pretreated liquid-based samples into processing vessels one by one, incubate the liquid-based samples at 37°C, switch the reaction system, wash red blood cells to remove them, change PBS buffer, add magnetic beads to remove white blood cell interference, etc. Treatment; add cell pre...

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Abstract

The invention discloses a single-cell slide preparation method based on liquid-based cell slide preparation. The method comprises the following step of liquid-based sample treatment: putting pretreated liquid-based samples into a treatment vessel one by one, and carrying out 37 DEG C incubation, reaction system switching, red blood cell flushing removal, PBS buffer solution replacement, white blood cell interference removal by adding magnetic beads and the like on the liquid-based samples. In the invention, through technological processes of treating the liquid-based samples, adding a cell preservation treatment solution, precipitating, carrying out papanicolaou staining, and carrying out centrifugal slide preparation, problems that an existing single-cell slide preparation method is poorin slide preparation quality and is easy to lose effective diagnosis cells, smears are different in thickness, missed diagnosis is often generated, a large number of leukocytes and erythrocytes oftenremain in a sample, and observation is affected are solved; and the single-cell slide preparation method based on the liquid-based cell slide preparation is high in cell preservation stability, good in cell fixation, more complete in reserved cell form and capable of removing redundant cells.

Description

technical field [0001] The invention relates to the technical field of cytological detection, in particular to a single-cell sheet preparation method based on liquid-based cell sheet preparation. Background technique [0002] Cytology-based examination is to use liquid-based thin-layer cytology detection system to detect cervical cells and carry out cytological classification diagnosis. Compared with the traditional cervical smear and Pap smear, liquid-based cytology has significantly improved the satisfaction of samples and the detection rate of abnormal cervical cells. Liquid-based cytology is currently a relatively advanced cervical cancer in the world. Cytology techniques. [0003] Single-cell smearing method is a commonly used method, but the current single-cell smearing method is poor in quality, easy to lose effective diagnostic cells, smears vary in thickness, easy to miss diagnosis, and there are often a lot of residual cells in the sample. White blood cells and r...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/2813G01N1/30
Inventor 阎灼辉
Owner HANGZHOU JUNHUI BIOTECHNOLOGY CO LTD
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