Bionic artificial periosteum with "sandwich" structure and preparation method of bionic artificial periosteum

A technology of artificial bone and electrospun membrane, which can be used in tissue regeneration, pharmaceutical formulations, conjugated artificial filaments, etc., and can solve the problems of inability to simulate periosteal cell structure and single cell type.

Active Publication Date: 2021-09-10
北京市创伤骨科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method contains a single type of cells and cannot simulate the cellular structure of the different layers of the periosteum.

Method used

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  • Bionic artificial periosteum with "sandwich" structure and preparation method of bionic artificial periosteum
  • Bionic artificial periosteum with "sandwich" structure and preparation method of bionic artificial periosteum
  • Bionic artificial periosteum with "sandwich" structure and preparation method of bionic artificial periosteum

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Embodiment 1

[0053] Example 1. Preparation of artificial periosteum with bionic "sandwich" structure

[0054] (1) Preparation of fiber layer electrospun membrane

[0055] 1. Use a pipette to draw 20mL of hexafluoroisopropanol into a 50mL Erlenmeyer flask;

[0056] 2. Weigh 0.85g PLGA, add it to the above solvent, and stir it on a magnetic stirrer for 8 hours to dissolve it;

[0057] 3. Take by weighing 0.15g of collagen, add in the above solution system, continue to stir on the magnetic stirrer for 4h, make PLGA and collagen dissolve completely and form homogeneous solution, wherein, the mass content of PLGA is 4.25%, the mass content of collagen is 0.75%;

[0058] 4. Use a 6mL syringe to draw 6mL of the prepared spinning solution, and install the syringe, connecting pipe and nozzle at the correct position of the instrument, adjust the spinning parameters to 22kV, the receiving distance to 15cm, and the injection speed to 1.0mL / h, stop after 4 hours of continuous spinning, the diameter...

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Abstract

The invention discloses a bionic artificial periosteum with a "sandwich" structure and a preparation method of the bionic artificial periosteum. The bionic artificial periosteum comprises a fiber layer electrospinning membrane and a germinal layer electrospinning membrane, and at least one of the following cell sheet layers is laid between the fiber layer electrospinning membrane and the germinal layer electrospinning membrane to form the sandwich structure: an osteoblast precursor cell sheet layer, a mesenchymal stem cell sheet layer and a vascular endothelial cell sheet layer; at least one cell sheet layer is arranged; the fiber layer electrospinning membrane is a spinning membrane made of a degradable high polymer material and a natural high polymer material; the germinal layer electrospinning membrane is a compound of a natural high polymer material or natural high polymer material and a degradable high polymer material. According to the bionic artificial periosteum, the nanofiber membrane is compounded with the cell sheet layers, so that the strength of the cell sheet layers can be improved, the requirements of surgical operations such as suturing can be met, the cells can be fixed between the nanofiber membranes by utilizing the cell shielding effect of the nanofiber membrane, so that the cells are effectively prevented from diffusion after being implanted into a body, and the cell utilization rate is effectively improved.

Description

technical field [0001] The invention relates to a bionic "sandwich" structure artificial periosteum and a preparation method thereof, and relates to the research field of bone defect repair and regeneration. Background technique [0002] At present, periosteum transplantation is often used in clinical treatment to supplement the missing periosteum. Periosteal transplantation techniques include autologous periosteum transplantation, allogeneic periosteum transplantation and artificial periosteum transplantation. Autologous periosteum transplantation has problems such as secondary injury, insufficient periosteum at the donor site, and complications; while allogeneic periosteum has a large risk of pathogen carrying and immune rejection. Therefore, it is of great significance to develop a new type of artificial periosteum that can replace the human body's own periosteum. [0003] So far, no "artificial periosteum" product has been used clinically. The materials used to replace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/50A61L27/56A61L27/18A61L27/24A61L27/20A61L27/22D01F8/02D01F8/14D01F8/18D04H1/728D04H1/435D04H1/04D04H1/30
CPCA61L27/3821A61L27/3834A61L27/3808A61L27/50A61L27/56A61L27/18A61L27/24A61L27/20A61L27/222D01F8/02D01F8/14D01F8/18D04H1/728D04H1/435D04H1/04D04H1/30A61L2430/02A61L2400/12C08L67/04C08L5/08
Inventor 石锐张静爽
Owner 北京市创伤骨科研究所
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