36results about How to "Improve the efficiency of induced differentiation" patented technology

The present invention provides a cell culturing device that, when culturing cells using a cell culture bag in which a plurality of recesses is formed, releases cells or cell aggregates adhering to theinner surfaces of the recesses while preventing movement of cells between the recesses, thereby improving the proliferation / differentiation induction efficiency of cells or cell aggregates. The present invention is a cell culturing method that uses a cell culturing bag (1) that comprises a bag body (2) configured from an upper surface film (21) and a lower surface film (22) having a sealed periphery, and a port (3) attached to the bag body (2), a plurality of recesses (4) being formed in the lower surface film (22), and the method comprising: a closing-off step for discharging a culture medium (S) contained in the bag body (2) through the port (3), and closing off the plurality of recesses (4) using the upper surface film (21); and a releasing step for releasing, in some or all of the plurality of closed-off recesses (4), spheroids adhering to the inner surfaces of the recesses (4).

The invention discloses a culture solution for inducing mesenchymal stem cells to differentiate into islet-like cells, and an inducing method and application of the culture solution. The culture solution comprises the following materials: niacinamide, Conophylline, cell growth factor, betacellulin and a base medium. The base medium contains 97% of high glucose DMEM (Dulbecco Modified Eagle Medium), 2% of B-27 and 1% of N-2. The inducing method comprises the following steps of: preparing an inducing culture solution; preparing the human mesenchymal stem cells; taking the human mesenchymal stem cells, inoculating the human mesenchymal stem cells into a six-hole ultralow absorption culture plate by 1.5-2*105cells / hole, adding 3ml inducing culture medium into each hole and carrying out suspended induction; and changing liquid at every 3 days, collecting cell supernatant at the ninth day, and storing the cell supernatant at the temperature of -20 DEG C. The culture solution disclosed by the invention has the advantages that the human mesenchymal stem cells are induced to differentiate into the islet-like cells by utilizing the combination of the niacinamide and the Conophylline, so that the inducing cycle is shortened, the suspension cells are beneficial to being clustered to form cell clusters similar to natural islets, further the induced differentiation efficiency is obviously increased and the clinical application risk is reduced; and the function of inducing the secretion of the cell insulin is obviously improved.

The invention belongs to the field of biomedicine, and relates to an inducer for inducing mesenchymal stem cells to differentiate into islet cells. The invention discloses the inducer for inducing mesenchymal stem cells to be differentiated into islet cells. The inducer is prepared from the following components: GLP-1, parathyroid hormone, acetaminophen, rapamycin, icariin, trametinib, EPO and VEGF. According to the inducer for inducing and differentiating the mesenchymal stem cells into the islet cells, all the components are safe and non-toxic, the number of steps needed by induced differentiation is small, the time is short, and the induction efficiency is high.

The invention discloses a kit for verifying endodontium mesenchymal stem cells. The kit comprises a flow phenotype detection reagent set, cell fixation liquid, an ordinary culture medium, an adipogenesis induction and detection reagent set, an osteogenesis induction and detection reagent set and a chondrogenesis induction and detection reagent set. Usually, conventional osteogenesis induction and adipogenesis induction need about 24 days, consumed time is long, and induction efficiency is low; by the kit, the time for osteogenesis induction and adipogenesis induction is shortened to 18 days, induction efficiency is improved, and induction time is shortened; conventional flow detection mainly aims at universal surface markers of mesenchymal stem cells, but the endodontium mesenchymal stem cells have positively-expressed surface markers CD146 which are different from the surface markers of other mesenchymal stem cells; CD146 antibodies are added into the kit, and accordingly, accurate verification results can be acquired.

The invention belongs to the technical field of induction and differentiation of stem cells, and in particular relates to an inducer of directed differentiation of mesenchymal stem cells. A mesenchymal stem cell epidermal differentiation inducer, which is composed of KGF, BMP‑4, PDGF‑AB, VEGF, IL‑2, and Forskolin. After adding complete medium, the concentration of each component is KGF 20~30μg / L , BMP-4 5-10 μg / L, PDGF-AB 5-10 μg / L, VEGF 10-20 μg / L, IL-2 5-10 μg / L, Forskolin 1-2 mg / L. The mesenchymal stem cell epidermal differentiation inducer of the present invention uses cytokines combined with small molecule compounds to efficiently induce mesenchymal stem cells to differentiate into epidermal cells, greatly improves the differentiation efficiency, and has strong cell activity after induction without cell transfection. Therefore, there is no genetic change and risk of cancer, no ethical issues, and high safety.

The invention discloses a method for inducing differentiation of human skin-derived precursor cells into corneal endothelial-like cells. The present invention successfully induces corneal endothelial-like cells that are theoretically close to normal human corneal endothelial cells by using human skin-derived precursor cells through co-culture with corneal endothelial cells B4G12, and applies the obtained corneal endothelial-like cells to corneal endothelial cells The decompensated animal model successfully repairs the corneal endothelial layer of animals, which has important clinical application prospects.