Method for Rapid Propagation of Job's tears Tissue Culture
A technology of tissue culture and Coix coix, applied in the field of plant cultivation, can solve the problems of single species, low efficiency of callus induction and differentiation, serious diseases, etc., and achieve good effect and high efficiency of callus induction and differentiation
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Embodiment 1
[0012] Embodiment 1: carry out according to the following steps:
[0013] 1. Select coix seeds as explants and carry out disinfection treatment. The disinfection treatment is to soak in 75% alcohol for 1 minute, rinse with sterile water twice, add 0.1% mercury liter for 15 minutes, and rinse with sterile water for 3 times.
[0014] 2. Put the sterilized Coix explants into the Coix callus differentiation medium for culture and differentiation. The Coix coix callus differentiation medium is MS+2,4-D 3.0mg / L+6-BA1.0mg / L .
[0015] 3. By cultivating the coix callus, the subculture of the coix coix callus is strengthened at the same time, and the subculture medium of the coix coix callus is MS+2, 4D 2.0mg / L+6-BA 0.5mg / L.
[0016] 4. The best culture of coix redifferentiation is carried out through subculture of coix coix callus. The adopted coix coix redifferentiation medium is MS+6-BA 4.0mg / L+2,4D 0.4mg / L.
[0017] 5. Through the re-differentiation of coix coix, when coix coix c...
Embodiment 2
[0019] Embodiment 2: carry out according to the following steps:
[0020] 1. Select coix seeds as explants and carry out disinfection treatment. The disinfection treatment is to soak in 75% alcohol for 1 minute, rinse with sterile water for 3 times, add 10% sodium hypochlorite to soak for 20 minutes, and rinse with sterile water for 5 times.
[0021] 2. Put the sterilized Coix explants into the Coix callus differentiation medium for culture and differentiation. The Coix coix callus differentiation medium is MS+2,4-D 3.0mg / L+6-BA1.0mg / L .
[0022] 3. By cultivating the coix callus, the subculture of the coix coix callus is strengthened at the same time, and the subculture medium of the coix coix callus is MS+2, 4D 2.0mg / L+6-BA 0.5mg / L.
[0023] 4. The redifferentiation culture of Coix is carried out by subculture of Coix callus, and the adopted Coix redifferentiation medium is MS+6-BA 4.0 mg / L+2,4D 0.4 mg / L.
[0024] 5. Through the re-differentiation of coix coix, when coix...
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