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Serum-free induction medium and induction method for differentiating iPS (induced pluripotent stem) cell into endodermal progenitor cell

A technology for inducing culture medium and cell differentiation, applied in the field of serum-free induction medium, it can solve the problems of low induction efficiency of simple cytokines and low directional differentiation rate, and achieves a high-efficiency induction and differentiation efficiency, high reliability, and improved safety. Effect

Active Publication Date: 2017-05-31
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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Problems solved by technology

However, the induction efficiency of simple cytokines is low, the co-culture induction has immunogenicity, the embryoid body has the characteristics of induction of three germ layers, and the directional differentiation rate is low

Method used

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  • Serum-free induction medium and induction method for differentiating iPS (induced pluripotent stem) cell into endodermal progenitor cell
  • Serum-free induction medium and induction method for differentiating iPS (induced pluripotent stem) cell into endodermal progenitor cell
  • Serum-free induction medium and induction method for differentiating iPS (induced pluripotent stem) cell into endodermal progenitor cell

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Embodiment Construction

[0017] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of ...

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Abstract

The invention discloses a serum-free induction medium and an induction method for differentiating an iPS (induced pluripotent stem) cell into an endodermal progenitor cell. The serum-free induction medium comprises a first-stage medium, a second-stage medium and a third-stage medium. According to the serum-free induction medium and the induction method for differentiating the iPS cell into the endodermal progenitor cell, the iPS cell can be directionally induced to be differentiated into the endodermal progenitor cell, thereby providing a technical support for each iPS cell induced to be differentiated into an adult. In addition, no serum is used by the serum-free induction medium and the induction method, so that risks brought by animal-derived pathogens are effectively avoided, and the clinical application safety is improved.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a serum-free induction medium and an induction method for iPS cells to differentiate into endoderm progenitor cells. Background technique [0002] Tissue engineering has been a research hotspot in recent years, but it is difficult for seed cells to be cultured and expanded on a large scale in vitro, and the source of seed cells hinders the development of tissue engineering. The sources of autologous seed cells are limited, allogeneic seed cells have rejection reactions, and embryonic stem cells have ethical issues. Therefore, urine cells are collected from urine and induced into iPS cells (induced pluripotent stem cells). iPS cells Re-induction into individual adult cells can solve the problem of seed source for tissue engineering. Obtaining cells from urine is convenient and quick, without trauma, and has a wide range of sources. [0003] At present, the methods of inducing t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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