The invention relates to the technical field of medical diagnosis, and discloses a cardiovascular and cerebrovascular and diabetes related four-high index composite quality control product and a preparation method thereof. The composite quality control product comprises a matrix liquid, a reducing agent, bovine serum albumin, an anticoagulant, polyol, uric acid, homocysteine, glucose, low-densitylipoprotein cholesterol and a preservative. The composite quality control product which can be used for testing four cardiovascular and cerebrovascular and diabetic indexes of Glu, Hcy, UA and LDL-C at the same time can be prepared at a time, healthy human plasma is selected as a matrix liquid raw material, the components are basically consistent with those of serum after treatment, and the matrixeffect is reduced to the maximum extent; meanwhile, by matching with other components, the prepared four-high quality control product has good uniformity and stability, stable performance, long preservation time and simplicity and convenience in use, meanwhile, the accuracy of quality control during joint inspection is greatly improved, and a more accurate basis is provided for clinical diagnosisof cardiovascular and cerebrovascular diseases and diabetes related diseases.