A kind of cell preservation solution and its application in flow cytometry sample preservation

Abstract

The invention provides the application of a cell preservation liquid in flow cytometry detection. During detection, the typing of leukemia and lymphoma is completed. The cell preservation liquid comprises Na2HPO4, KH2PO4, NaCl, KCl, polyethylene glycol, chromium chloride and any optionally selected anticoagulant. In addition, the invention also discloses a method for preparing the cell preservation liquid and a method for preserving a flow-type cell sample. The cell preservation liquid can be used for the long-time and stable preservation of clinical cell samples. Especially for flow-type cell samples, the long-term and stable preservation effect is realized.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular, the invention relates to a preservation method of a cell-containing sample detected by a flow cytometer. Background technique [0002] With the development of biological and medical technology, scientific research detection and clinical diagnosis have gradually risen to the level of cells and genes. As one of the important means of quantitative analysis of single cells or biological ions, flow cytometry is also widely used. The working principle of flow cytometry is the multi-parameter and rapid quantitative analysis of single cells or other biological particles by monoclonal antibodies at the cellular and molecular level. It can analyze tens of thousands of cells at high speed, and can measure multiple parameters from one cell at the same time. It has the advantages of fast speed, high precision and good accuracy. It is one of the most advanced cell quantitative analysis tech...

AI Technical Summary

Problems solved by technology

[0003] However, there are few studies on the samples for flow cytometry and their storage / processing methods in the prior art

U.S. Patent No. 5,753,428A discloses a cell preservation solution, but the inventors have found that, due to the use of citrate and other substances, the markers such as CD33 and CD13 on the blood sample are unstable, and the sample time preserved by it is slightly longer. Flow cytometric testing as described in Chinese Patent No. 201610027214 will produce different results from the original sample testing

There are few domestic studies on this. For example, Chinese Patent No. 201410534999 discloses a reagent for stabilizing blood sample cells. Although it claims to be used for flow cytometry detection, as an industry insider, it uses Potassium dichromate of strong zinc oxide, its preservation effect is not as good as the commonly used cell preservation solution containing paraformaldehyde

[0004] The inventor found that, especially the flow cytometry test described in the inventor's Chinese Patent No. 201610027214 involves the interpretation of the results by antibodies, so if the test cannot be performed in time, or the sample is processed in a suitable way, it will cause antigen expression A disproportionate decrease in intensity can greatly reduce the accuracy of the results and can even lead to misdiagnosis of leukemia / lymphoma immunophenotyping cases

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