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Non-cervical exfoliated cell preserving solution with pretreatment function and application thereof

A technology of exfoliated cells and preservation solution, which is applied in the field of cervical exfoliated cell preservation solution, which can solve the problems of many overlapping and grouping of cells, easily lead to misjudgment, affect cell precipitation and absorption, etc., and achieve mucus processing ability and blood cell disintegration ability Strong, clear film background, easy to read and diagnose the effect

Inactive Publication Date: 2019-10-22
南京福怡科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The mucus in the mucus sample affects the precipitation and absorption of cells, which may easily cause false negatives;
[0007] 2. Bloody samples were not processed thoroughly, and red blood cells interfered with interpretation;
[0008] 3. Cells dissolve or swell, and the cell preservation effect is poor, which may easily lead to misjudgment;
[0009] 4. There are many overlapping and grouping of cells, and the production effect cannot be evenly tiled.
[0010] In addition, imported preservation solutions are often used in clinical practice, which is costly and unfavorable for popularization and promotion.

Method used

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  • Non-cervical exfoliated cell preserving solution with pretreatment function and application thereof
  • Non-cervical exfoliated cell preserving solution with pretreatment function and application thereof
  • Non-cervical exfoliated cell preserving solution with pretreatment function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preservation solution in this embodiment contains the following components: 45% methanol, 39% Tris buffered saline solution, 5% sodium chloride, 2% formaldehyde solution, 2% dithiothreitol, 2% glacial acetic acid, erythrocyte lysate 5%.

[0032] In this embodiment, the application of the cell preservation solution and the preparation method are as follows:

[0033] 1. Take 15ml of preservation solution and put it in a 50ml preservation solution bottle for later use;

[0034] 2. Submit 250ml of pleural effusion sample, centrifuge twice to remove the supernatant and put it in the preservation solution bottle;

[0035] 3. After shaking for 5 minutes, transfer to a test tube and centrifuge at 2000 R / min for 5 minutes;

[0036] 4. Remove the supernatant and adjust the buffer to 4ml cell suspension;

[0037] 5. Take 1ml of the cell suspension to the film chamber, and spin the film by centrifugation;

[0038] 6. Take out the slides for HE staining process, and after st...

Embodiment 2

[0041] The preservation solution in this example contains the following components: methanol 50%, phosphate buffered saline 26%, sodium chloride 15%, β-propiolactone 5.5%, glacial acetic acid 1.5%, red blood cell lysate 2%.

[0042] In this embodiment, the application of the cell preservation solution and the preparation method are as follows:

[0043] 1. Take 15ml of preservation solution and put it in a 50ml preservation solution bottle for later use;

[0044] 2. Put the sputum sample for inspection into the above-mentioned preservation solution bottle;

[0045] 3. After shaking for 8 minutes, transfer to a centrifuge tube and centrifuge at 2000 R / min for 6 minutes;

[0046] 4. Remove the supernatant and adjust the buffer to 4ml cell suspension;

[0047] 5. Draw 1.2ml of cell suspension to the production chamber, and naturally settle for production;

[0048] 6. Take out the slides for Papanicolaou cytology staining process, and after staining, detect and interpret the cel...

Embodiment 3

[0051] The preservation solution in this example contains the following components: 30% methanol, 20% ethanol, 20% Tris buffered saline, 23% phosphate buffered saline, 2% sodium chloride, 1% formaldehyde, 1% glacial acetic acid, red blood cells Lysis buffer 3%.

[0052] In this embodiment, the application of the cell preservation solution and the preparation method are as follows:

[0053] 1. Take 15ml of preservation solution and put it in a 50ml preservation bottle for later use;

[0054] 2. Thyroid puncture samples are placed in the above preservation solution bottle;

[0055] 3. After shaking for 6 minutes, transfer to a centrifuge tube and centrifuge at 2000 R / min for 5 minutes;

[0056] 4. Remove the supernatant and adjust the buffer to 4ml cell suspension;

[0057] 5. Draw 0.5ml of the cell suspension to the production chamber, and naturally settle to produce the film;

[0058] 6. Take out the slides for HE staining process, and after staining, detect and interpret ...

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Abstract

The invention relates to a non-cervical exfoliative cell preserving solution with a pretreatment function and application thereof, in particular to a non-cervical exfoliated cell preserving solution containing a cell fixative, a cleaning solution, an osmotic pressure maintenance agent, a microbial inactivating agent, and a cell lysis component.Thenon-cervical exfoliative cell preserving solution canbetter preserve non-cervical exfoliated cells comprising mucus samples, body fluid samples, puncture and brush samples to protect cell integrity and fixation. At the same time, various parts of thecells can be easily colored to be suitable for observation, long-term preservation, and analysis, and the preserved cells can also be used for cell wax blockmaking and immunohistochemical detection.

Description

technical field [0001] The invention relates to a non-cervical exfoliated cell preservation solution with pretreatment function and its application. Background technique [0002] The function of the preservation solution is to protect the integrity and fixity of the exfoliated cells. The purpose of preserving the cells is to preserve the original structure of the cells as completely as possible, coagulate or precipitate the components in the cells, and avoid the degradation, autolysis, and corruption of the cells. And deformation, etc., inactivate various enzymes in the cell, prevent the denaturation and dissociation of various molecules of the cell, and will not change and damage in the subsequent processing and production process. At the same time, each part of the cell can be easily colored, which is suitable for observation, long-term storage and analysis. [0003] Traditional non-gynecological samples are unstable in preservation or even directly smeared without preser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02G01N1/28G01N1/30
CPCA01N1/021A01N1/0226G01N1/2813G01N1/30
Inventor 姚斌左露露
Owner 南京福怡科技发展股份有限公司
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