39 results about "Cell integrity" patented technology

A novel reagent system for use with automated and semi-automated hematology analyzers including an essentially isotonic blood diluting reagent, a blood cell lysing and hemoglobin conversion reagent, and a second lysing reagent for differentiating white blood cells into classes by size and functional characteristics. The diluent reagent enhances properties for counting and sizing blood specimens, while stabilizing cellular volume and cellular integrity for many hours. The blood cell lysing reagent removes red blood cells and enables subsequent enumeration of white blood cells and simultaneous determination of hemoglobin without use of the toxic cyanide anion. The third lysing reagent and a companion quenching differentiates blood cells into classes by size and functional characteristics, based on d.c. impedance volume, conductivity/opacity and light scatter measurements. The companion quenching reagent adjusts pH and conductivity of the final measurement solution to match the analyzer system requirements. Novel methods for use of the reagents with automated and semi-automated hematology analyzers are also provided.</PTEXT>

The technology disclosed relates to granular analysis of design data used to prepare chip designs for manufacturing and to identification of similarities and differences among parts of design data files. In particular, it relates to parsing data and organizing into canonical forms, digesting the canonical forms, and comparing digests of design data from different sources, such as designs and libraries of design templates. Organizing the design data into canonical forms generally reduces the sensitivity of data analysis to variations in data that have no functional impact on the design. The details of the granular analysis vary among design languages used to represent aspects of a design. For various design languages, granular analysis includes partitioning design files by header/cell portions, by separate handling of comments, by functionally significant/non-significant data, by whitespace/non-whitespace, and by layer within a unit of design data. The similarities and differences of interest depend on the purpose of the granular analysis. The comparisons are useful in many ways.

The technology disclosed relates to granular analysis of design data used to prepare chip designs for manufacturing and to identification of similarities and differences among parts of design data files. In particular, it relates to parsing data and organizing into canonical forms, digesting the canonical forms, and comparing digests of design data from different sources, such as designs and libraries of design templates. Organizing the design data into canonical forms generally reduces the sensitivity of data analysis to variations in data that have no functional impact on the design. The details of the granular analysis vary among design languages used to represent aspects of a design. For various design languages, granular analysis includes partitioning design files by header/cell portions, by separate handling of comments, by functionally significant/non-significant data, by whitespace/non-whitespace, and by layer within a unit of design data. The similarities and differences of interest depend on the purpose of the granular analysis. The comparisons are useful in many ways.

The technology disclosed relates to granular analysis of design data used to prepare chip designs for manufacturing and to identification of similarities and differences among parts of design data files. In particular, it relates to parsing data and organizing into canonical forms, digesting the canonical forms, and comparing digests of design data from different sources, such as designs and libraries of design templates. Organizing the design data into canonical forms generally reduces the sensitivity of data analysis to variations in data that have no functional impact on the design. The details of the granular analysis vary among design languages used to represent aspects of a design. For various design languages, granular analysis includes partitioning design files by header/cell portions, by separate handling of comments, by functionally significant/non-significant data, by whitespace/non-whitespace, and by layer within a unit of design data. The similarities and differences of interest depend on the purpose of the granular analysis. The comparisons are useful in many ways.

The invention relates to a human retinal pigment epithelial cell separation and cryopreservation method which includes the steps: firstly, cleaning and washing retinal pigment epithelial cells by nutrient solution; secondly, mixing Dispase II separase and DPBS buffer solution according to the ratio of 1:(40-50)w/v to prepare Dispase II separase solution; thirdly, mixing the Dispase II separase solution and Tryple select solution according to the volume ratio of 1:(2-3) to prepare digestive fluid for digestive separation; finally, performing cryopreservation after counting and centrifuging. The separation method is simple, the steps are easily controlled, the digestive fluid prepared by mixing the Dispase II separase solution with the Tryple select solution is used for performing digestive separation for the retinal pigment epithelial cells, adherence removing time of the retinal pigment epithelial cells can be shortened, cell integrity is kept, and cell injury is greatly reduced.

This invention is directed to methods of preventing or treating diseases or conditions associated with alterations in cellular integrity including alterations in endothelial permeability, excessive cell proliferation or tissue remodeling. Particularly, this invention is directed to methods of treating diabetic nephropathy, malaria, or cancer. The method comprises identifying a subject in need of the treatment, and administering to the subject an effective amount of a novel rho kinase inhibitor compound to treat the disease.

A composition suitable for inhibiting the outgrowth of fungi is provided comprising a first ingredient which inhibits the biogenesis of a normal fungal cell wall and a second ingredient which is capable of perturbing the structure of the cellular membrane of said fungi, so that either the cellular integrity is essentially lost or cell division cannot take place, or both. The first ingredient preferably inhibits the anchorage of cell wall proteins in the cell wall of the fungi and is suitably a beta-(1,6)-glucose polysaccharide, preferably beta-gentiobiose or a pustulan fragment. The second ingredient is suitably a natural microbial membrane affecting substance (MMAS), preferably MB-21. The composition is particularly suitable as a preservative for inhibiting the outgrowth of fungi in food products, such as sauces, dressings, ketchups, and soups, but is also useful for preventing or inhibiting undesired fungal growth on other products such as personal health care products, e.g. soap bars.

The invention discloses a cell freezing solution for preparing a dry tumor cell quality control and application of the cell freezing solution. According to a formula, the cell freezing solution is prepared by adding 5-25% of protein, 3-15% of a cell stabilizing agent A, 1-10% of a cell stabilizing agent B and 0.01-0.1% of a bacteriostatic agent into PBS (phosphate buffer solution). The cell freezing solution successfully solves the problems that a cell integrity rate after freeze-drying is very low or active ingredients of cell membrane structural proteins and cell contents cannot be reservedwell in the existing cell freeze-drying technology. A preparation method of the dry tumor cell quality control directly uses human-derived tumor cell strains containing cell membrane structures and contents to prepare the quality control; the quality control is derived from human tumor cells and has the same characteristics as those of similar tumor cells in clinical liquid biopsy detection samples; and the quality control prepared by the method is applicable to the quality control of clinical liquid biopsy and can promote extensive popularization and application of the clinical liquid biopsy.

The invention discloses application of a lanthanum compound to bacterium growth promotion. The lanthanum compound is La(NO3)3.6H2O; the addition concentration of the lanthanum compound is 5.0 to 200.0mg/L; the lanthanum compound can be used for promoting bacterial reproduction, and concretely protects bacterium cytoderm peptidoglycan structures and beta-1,4 glycosidic bonds; and the bacteria are escherichia coli. The acting target points of Penicillin and Lysozyme are used as the references; the effect that the adjacent polysaccharide chains in the cytoderm are crosslinked to protect the cytoderm peptidoglycan through the physiological effect of the La(NO3)3.6H2O capable of reversing Penicillin is discovered; the cytoderm beta-1,4 glycosidic bonds are protected through the physiological effect for reversing Lysozyme; the cell completeness is enhanced; and the bacterial reproduction is promoted.

The invention relates to an angiogenesis inhibition drug. The angiogenesis inhibition drug has a structural formula shown in the following description. A result of a research on zebrafish embryonic development shows that the angiogenesis inhibition compound belongs to a Src kinase inhibitor, through VEGFR2 and MAP kinase approach inhibition, vascular endothelial cell integrity is interfered so that small blood vessel lumen diameters are gradually reduced and blood vessel endothelium is broken finally and thus the angiogenesis inhibition drug belongs to an angiogenesis inhibition compound.

Methods and apparatus for separation of rare cells of interest from a biological sample are provided. The present methods and apparatus effectively reduce fragile rare cell exposure to manipulations that adversely impact cell integrity and collection.

The invention discloses a non-water leucorrhea specimen storing liquid and a preparation method and pH value test collection tube of the liquid. The non-water leucorrhea specimen storing liquid comprises the following substances by weight percent: 96.5wt% of anhydrous glycerin, 0.2wt% of polyethyleneglycol 8000 as a surface active agent, 0.25wt% of bromocresol green, 0.05wt% of methyl red, 2.0wt% of absolute ethyl alcohol and 1.0wt% of mercapto sodium acetate. The non-water leucorrhea specimen storing liquid has the advantages that visible components such as bacteriums, cells, trichomonads and sick bodies of the leucorrhea can be protected by anhydrous glycerin (glycerol) when being frozen at a temperature less than -20 DEG C; the cell completeness of the visible components such as cells, bacteriums, trichomonads and the like can still be maintained after being redissolved without changing the morphology under a microscope; the leucorrhea can be solved in water in any concentration when needing to be diluted; mercapto sodium acetate is used as a reducing agent to prevent the specimen from inactivation caused by dehydration, oxidation and autolysis during the storage or transportation; and the non-water leucorrhea specimen storing liquid does not affect the cell lysis and determination of Chlamydia trachomatis or various causative agents of the leucorrhea.