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Harvesting method for large-scale production of toxic dinoflagellate

A harvesting method and dinoflagellate technology are applied in the harvesting field of large-scale production of toxic dinoflagellates, can solve the problems of high energy consumption, high investment, large consumption, etc., and achieve the effects of low cost, improved purification speed, and simple operation.

Inactive Publication Date: 2009-11-25
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of flocculant and centrifugation method can not only avoid the problem of excessive use of flocculant alone, and the leakage of dinotoxin in the content caused by cell rupture, but also solve the high investment and high energy consumption of large-scale production. problem, and the application of centrifugal method has realized large-scale harvesting in large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Harvesting of poisonous dinoflagellate Coolia monotis

[0036] The harvesting of the poisonous dinoflagellate Coolia monotis of present embodiment, its concrete steps are as follows:

[0037] (1) When the toxic dinoflagellate Coolia monotis grows to the harvest period in the water body to be cultivated, flocculant KA1 (SO 4 ) 212H 2 O to KA1 (SO 4 ) 2 12H 2 The final concentration of O is 0.3 mass ‰, stirring while adding, so that a large number of algae cells are precipitated;

[0038] (2) Stand still for 2 hours, microscopic inspection, the upper water body is basically free of dinoflagellate cells, drain the supernatant water body, and collect the sediment;

[0039] (3) Centrifuge the above precipitated solution at a speed of 2000r / min for 15 minutes, discard the supernatant, and the sediment at the bottom is the algae mud required in this example, collect the algae mud and put it in the refrigerator for later use.

[0040] (4) Contrast test: KA1(...

Embodiment 2

[0042] Example 2 Harvesting of poisonous dinoflagellate Prorocentrium lima

[0043] The gathering of the poisonous dinoflagellate Prorocentrium lima of present embodiment, its concrete steps are as follows:

[0044] (1) The toxic dinoflagellate Prorocentrium lima in the water body to be cultivated grows to the harvest period, and the flocculant KA1 (SO 4 ) 2 12H 2 O to KA1 (SO 4 ) 2 12H 2 The final concentration of O is 0.2‰, stirring while adding, so that a large number of algae cells are precipitated;

[0045] (2) Stand still for 1 hour, microscopic inspection, the upper water body is basically free of dinoflagellate cells, drain the supernatant water body, and collect the sediment;

[0046] (3) Centrifuge the above precipitated solution at a speed of 2000r / min for 10 minutes, discard the supernatant, and the sediment at the bottom is the algae mud required in this example, collect the algae mud and put it in the refrigerator for later use.

[0047] (4) Contrast test:...

Embodiment 3

[0048] Example 3 Harvesting of toxic dinoflagellate Prorocentrum rhathymum

[0049] The harvesting of the poisonous dinoflagellate Prorocentrum rhathymum of the present embodiment, its concrete steps are as follows:

[0050] (1) The toxic dinoflagellate Prorocentrum rhathymum grows to the harvest period in the water body to be cultivated, and the flocculant KA1 (SO 4 ) 2 12H 2 O to KA1 (SO 4 ) 2 12H 2 The final concentration of O is 0.25‰, stirring while adding, so that a large number of algae cells are precipitated;

[0051] (2) Stand still for 2 hours, microscopic inspection, the upper water body is basically free of dinoflagellate cells, drain the supernatant water body, and collect the sediment;

[0052] (3) Centrifuge the above precipitated solution at a speed of 2000r / min for 15 minutes, discard the supernatant, and the sediment at the bottom is the algae mud required in this example, collect the algae mud and put it in the refrigerator for later use.

[0053] (4)...

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Abstract

The present invention discloses a harvesting method for a large-scale production of a toxic dinoflagellate, employing a flocculating agent KAL(SO4)2 12H2O to precipitate the toxic dinoflagellate cells, and obtaining a toxic dinoflagellate body by a centrifugal collection. The method provides a appropriate flocculating agent dosage for different toxic dinoflagellate and is capable of avoiding the flocculating agent dosage from being oversize and preventing a cell rupture content object toxin from leaking outside by combining a centrifugal method, it is also capable of avoiding a problem that the cell will not be precipitated due to less flocculating agent dosage and avoiding a high-cost high energy consumption collection by the centrifugal method only. After precipitating the dinoflagellate cell by the flocculating agent, further the centrifugal method is selected to purify to obtain a algae mud, wherein the centrifugation is capable of improving a purifying speed of a large-scale production and purifying a good deal of precipitating liquid of the large-scale production. The method of the invention is capable of harvesting a good deal of toxic dinoflagellate safely and highly effectively without damage of the cell integrity, thereby an active secondary metabolic product of the dinoflagellate toxin is extracted.

Description

technical field [0001] The invention relates to the field of algae harvesting, in particular to a harvesting method for large-scale production of toxic dinoflagellates. Background technique [0002] Marine dinoflagellate, also known as marine dinoflagellate, is one of the main marine red tide organisms. The toxic secondary metabolites produced by dinoflagellate are also called dinoflagellates. Studies have shown that dinoflagellates have very broad application prospects in the development of various therapeutic drugs such as anticancer, analgesic, anesthesia, antiviral, and antibacterial, as well as pharmacological research tools such as cancer. [0003] Due to the very low cell density of toxic dinoflagellates in the natural environment, the difficulty in the source of toxin raw materials restricts further in-depth research and application of toxins. At present, there are few types of toxins in the international market and the prices are expensive. The molecular weight of ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 龙丽娟梁计林张偲吴军何伟宏李庆欣
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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