Method for treating externally-autoplastic autogenous skull sheet

Abstract

The invention relates to the field of biology materials, in particular to a method for treating an externally-autoplastic autogenous skull sheet, which includes: 1 freezing fresh skull flap for 24 hours at temperature of -10 DEG C, rewarming for 48 hours in natural environment (about 20 DEG C) so as to break integrity of cells and lead the cells to be self-dissolved fully; 2 removing soft tissue on the surface of skull manually; 3 drilling holes on the skull in mesh mode and leading areas among holes to be 1cm*1cm; 4 soaking distilled water and trypsin for 48 hours at the temperature of 37 DEG C, changing the distilled water every 24 hours so as to fully precipitine an autogenous solution; and 5 soaking for 72 hours in medical hydrogen dioxide, changing the hydrogen dioxide every 24 hours to fully remove proteins in diploe; and soaking for 12 hours in the distilled water and then draining when no adverse reactions including infection, effusion and the like appear. Aiming at the problem that ankle joints are movable so that needle heads on the foot portion can move easily, the method combines special structure of the foot portion for fixing the foot portion so as to prevent liquid medicine from permeating into subcutaneous tissues.

Description

[0001] technical field [0002] The invention relates to the field of biomaterials, in particular to a processing method of bone materials that can be implanted in the body. [0003] Background technique [0004] In order to save the patient's life, neurosurgery often removes the patient's skull flap to reduce the patient's intracranial pressure and relieve the state of brain herniation. After the patient's condition is stable, the skull repair operation is performed. Currently commonly used repair materials generally use artificial materials (such as titanium alloy mesh) and autologous bone flap. The popularization and use of artificial materials is limited due to their deficiencies in shaping, pressure resistance, impact resistance, temperature insulation, rejection, and high price (about 20,000 yuan per repair material). The advantages of autogenous skull repair are difficult to achieve with other repair materials. The physical characteristics of the skull flap fully m...

AI Technical Summary

Problems solved by technology

The popularization and use of artificial materials is limited due to their deficiencies in shaping, pressure resistance, impact resistance, temperature insulation, rejection, and high price (about 20,000 yuan per repair material).

The advantages of autogenous skull repair are difficult to achieve with other repair materials. The physical characteristics of the skull flap fully meet the anatomical and physiological needs of the human body, and the bone induction activity is good. Comparatively speaking, it is the most ideal repair material, but it can meet the requirements of clinical application. There are large technical obstacles in the preparation of autologous skull flap repair materials, which limit its application; there are two methods of preservation of autologous skull flap: in vivo or in vitro

The disadvantage of in vivo preservation is that the trauma is large, the bone flap can be absorbed and becomes smaller, and osteoporosis often causes infection, ulceration, erosion, etc. at the bone flap embedding site, which leads to the failure of clinical application; in vitro preservation methods include liquid nitrogen cryopreservation, high temperature Disinfection, radiation disinfection and other methods; liquid nitrogen low-temperature cold storage has expensive equipment, easy pollution during operation, and many problems such as the human body’s immune response to residual organic substances after replantation, which are not easy to promote; the strength of the skull after high-temperature disinfection is significantly reduced , cannot fully meet clinical needs; the method of irradiation disinfection requires specialized industrial irradiation equipment, which is difficult to configure in hospitals, and cannot improve the immune response of the human body to residual organic substances after skull slices are replanted

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