343 results about "Cellular membrane" patented technology

The invention relates generally to intradermal, transdermal, and/or transmembrane delivery of biologically active substances in the epidermis and/or through the skin, sub-dermal tissues, blood vessels and cellular membranes without causing damage to the cellular surface, tissue or membrane. The biologically active substances may have a molecular weight no less than about 5.8 kDa to about 2,500 kDa, such as Hyaluronic Acid (HA). The biologically active substances may be deposited in a dermal patch containing a red algae polysaccharide-based matrix, wherein the red algae polysaccharide is an extract of Chondrus crispus at 2% by weight of the dermal patch. The invention provides systems and methods for enhanced intradermal, transdermal, and/or transmembrane delivery of such biologically active substances using pulsed incoherent light. The invention further provides a device for the application of the pulsed incoherent light to cellular surfaces and membranes using those compositions and methods.

Provided are electrokinetically-altered fluids (e.g., electrokinetically-altered gas-enriched fluids and solutions) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient for treating an inflammatory neurodegenerative condition or disease (e.g., a taupathy) or at least one symptom thereof. The electrokinetically-altered fluids or therapeutic compositions and methods include electrokinetically-altered ionic aqueous fluids optionally in combination with other therapeutic agents. Particular aspects provide for modulating phosphorylation of tau protein. Particular aspects provide for regulating or modulating intracellular signal transduction associated with said inflammatory responses by modulation of at least one of cellular membrane potential and/or conductance, membrane proteins such as membrane receptors, including but not limited to G-Protein Coupled Receptors (GPCR), and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes). Other embodiments include particular routes of administration or formulations for the electrokinetically-altered fluids and therapeutic compositions.

The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed.

Methods and compositions are provided for the persistent modification of cell membranes with exogenous proteins so as to alter the function of the cell to achieve effects similar to those of gene therapy, without the introduction of exogenous DNA. DNA sequences, the proteins and polypeptides embodying these sequences are disclosed for modulating the immune system. The modulations include down-regulation, up-regulation and apoptosis.

A microfluidic system for causing perturbations in a cell membrane includes (a) a microfluidic channel defining a lumen and configured such that a cell suspended in a buffer can pass there through, and (b) source or emitter of an energy field. The microfluidic channel may include a cell-deforming constriction. A diameter of the constriction may be a function of the diameter of the cell. Related apparatus, systems, techniques, and articles are also described.

Provided are compositions and methods for treating lung or respiratory disorders or conditions characterized by airflow obstruction or limitation, or symptoms thereof (e g, asthma, rhinitis, allergic rhinitis, and chronic obstructive pulmonary disease (CaPO) and CaPO-associated conditions (e g, bronchitis, emphysema, asthma), emphysema, pneumonia, bronchitis, in-fluenza, SARS, tuberculosis, and whooping cough (pertussis), and the like) comprising administering a therapeutic composition comprising at least one electrokinetically altered fluid comprising an ionic aqueous solution of charge-stabilized oxygen containing nanostructures as disclosed herein, or comprising administering a nonelectrokinetic superoxygenated aqueous solution The methods preferably comprise regulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins (e g, membrane receptors, (e g, G protein-coupled receptors, and intercellular junctions)) Additional aspects include therapeutic compositions, and combination treatment methods comprising administration of electrokinetically generated fluid in combination with at least one additional therapeutic agent (e g, albuterol, etc).

The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.

The invention discloses a preparation method of a nanopore cellular membrane. The method comprises the following steps: preparing a 6mg/mL to 14mg/mL polymer solution by selecting a proper solvent; coating the polymer solution on the surface of a substrate and standing in a normal-temperature atmospheric environment with the relative humidity of 60% to 90% for 30 seconds to 4 minutes; and obtaining the cellular membrane with a nanopore structure after the complete volatilization of the solvent, wherein the pore diameter of the cellular membrane ranges from 10nm to 100nm; the solvent is selected from carbon disulfide, chloroform, dichloromethane or toluene; and the substrate is selected from a silicon slice, glass, a gold slice, a silver slice, a polyester film or a polyimide film. According to the method disclosed by the invention, the cellular membrane with the surface-layer nanopore structure is prepared via a spirogram method by only adopting the double-end based functionalized linear polystyrene. As a result, the smallest pore is prepared up to now via the spirogram method.

The invention relates to a method and device for obtaining the biophysical characteristics of an ultra-diffraction limit cell membrane micro-structure. The device comprises a laser device and a semiconductor laser device. The laser output path of the laser device sequentially comprises: a polarizer, a first convergent lens set, an aperture, a first reflector, a spatial light modulator, a third convergent lens, a light barrier, a second convergent lest set, and a dichroscope; the reflected light output path of the dichroscope sequentially comprises the objective lens and the objective table of an optical microscope; the transmission light output path of the dichroscope sequentially comprises a filter, a switchable reflector, a sixth convergent lens, and a CCD detector; the laser output path of the semiconductor laser device sequentially comprises a second reflector, a probe, which is driven by a three dimensional controller, and a position sensitive detector; and the CCD detector, the three dimensional controller, and the position sensitive detector are all connected to a data collecting and controlling system. The provided method and device can obtain the information of biophysical characteristics of an ultra-diffraction limit cell membrane micro-structure.

The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed. Nanoparticles having phospholipid coatings were found to display improved responses relative to nanoparticles having other coatings that do not promote localization or attraction to membranes.