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349 results about "Cellular membrane" patented technology
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Light-activated cation channel and uses thereof
ActiveUS20070054319A1Improve abilitiesOrganic active ingredientsSenses disorderCell membraneExcitable cell
The present invention provides compositions and methods for light-activated cation channel proteins and their uses within cell membranes and subcellular regions. The invention provides for proteins, nucleic acids, vectors and methods for genetically targeted expression of light-activated cation channels to specific cells or defined cell populations. In particular the invention provides millisecond-timescale temporal control of cation channels using moderate light intensities in cells, cell lines, transgenic animals, and humans. The invention provides for optically generating electrical spikes in nerve cells and other excitable cells useful for driving neuronal networks, drug screening, and therapy.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Biosensors for single cell and multi cell analysis
InactiveUS20030104512A1Microbiological testing/measurementBiological testingElectricityBiological cell
The present invention relates to a structure comprising a biological membrane and substrate with fluidic network, an array of membranes and an array of fluidic networks in substrate, a high throughput screen, methods for production of the membrane, substrate structure, and a method for interconnected array of substrate structures and a method for attaching membranes to structure, a method to electrically record events from the membranes and a method to screen large compound library using the array. More particularly, it relates to biological cells and artificial cell membranes adhered to the substrate with a high electrical resistivity seal, a method to manufacture array configuration of such substrates, and a method to screen compounds using the membrane receptors such as ion-channels, ion pumps, & receptors.
Owner:CYTOPLEX BIOSCI
Compositions and methods for treating asthma and other lung disorders
InactiveUS20100008997A1Powder deliveryOrganic active ingredientsObstructive Pulmonary DiseasesOxygen
Provided are compositions and methods for treating lung or respiratory disorders or conditions characterized by airflow obstruction or limitation, or a symptom thereof (e.g., asthma, rhinitis, allergic rhinitis, and chronic obstructive pulmonary disease (COPD) and COPD-associated conditions (e.g., bronchitis, emphysema, asthma), emphysema, pneumonia, bronchitis, influenza, SARS, tuberculosis, and whooping cough (pertussis), and the like) in a subject in need thereof by administering a therapeutic composition comprising at least one electrokinetically altered fluid (gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures as disclosed herein. In certain aspects, the methods comprise regulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins (e.g., membrane receptors, (e.g., to G protein coupled receptors, and intercellular junctions)). Additional aspects include therapeutic compositions, and combination treatment methods comprising administration of electrokinetically generated fluid in combination with at least one additional therapeutic agent.
Owner:REVALESIO CORP
Compositions and methods for treating cystic fibrosis
Particular aspects provide electrokinetically-generated fluids (e.g., electrokinetically-generated gas-enriched fluids and solutions), and therapeutic compositions and methods comprising use thereof in treating at least one symptom of cystic fibrosis. In particular embodiments, at least one symptom of cystic fibrosis treated by the present invention include inhibition of Pseudomonas infection, synergy with tobramycin (including TOBI) for use against bacterial infection, and synergy with a bronchiodilator. In particular embodiments, the electrokinetically-generated fluids or therapeutic compositions and methods comprise combination with other therapeutic agents (e.g., antibiotics, albuterol, budesonide, etc.). In certain aspects, the methods comprise regulating or modulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins such as membrane receptors, including but not limited to G protein coupled receptors, and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes).
Owner:REVALESIO CORP
System, Device and Method for Electroporation of Cells
ActiveUS20170283761A1Avoid blockingHigh strengthBioreactor/fermenter combinationsBiological substance pretreatmentsDrug compoundCell membrane
A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.
Owner:CYTEQUEST INC
Compositions and methods for treating asthma and other lung disorders
Provided are compositions and methods for treating or preventing lung or respiratory disorders or conditions characterized by airflow obstruction or limitation, or a symptom thereof (e.g., asthma, rhinitis, allergic rhinitis (e.g. nose respiratory tract), and chronic obstructive pulmonary disease (COPD) and COPD-associated conditions (e.g., bronchitis, emphysema, asthma), emphysema, pneumonia, bronchitis, influenza, SARS, tuberculosis, and whooping cough (pertussis), and the like) in a subject in need thereof by administering a therapeutic composition comprising at least one electrokinetically generated fluid (including gas-enriched electrokinetically generated fluids) as disclosed herein, the electrokinetically altered aqueous fluid suitable to alter cellular membrane structure or function sufficient to provide for modulation of intracellular signal transduction, wherein treating a lung disorder or a symptom thereof is thereby afforded. Additional aspects relate to therapeutic compositions, and combination treatment methods comprising administration of at least one electrokinetically generated fluid in combination with at least one additional therapeutic agent.
Owner:REVALESIO CORP
Delivery of large molecular weight biologically active substances
The invention relates generally to intradermal, transdermal, and / or transmembrane delivery of biologically active substances in the epidermis and / or through the skin, sub-dermal tissues, blood vessels and cellular membranes without causing damage to the cellular surface, tissue or membrane. The biologically active substances may have a molecular weight no less than about 5.8 kDa to about 2,500 kDa, such as Hyaluronic Acid (HA). The biologically active substances may be deposited in a dermal patch containing a red algae polysaccharide-based matrix, wherein the red algae polysaccharide is an extract of Chondrus crispus at 2% by weight of the dermal patch. The invention provides systems and methods for enhanced intradermal, transdermal, and / or transmembrane delivery of such biologically active substances using pulsed incoherent light. The invention further provides a device for the application of the pulsed incoherent light to cellular surfaces and membranes using those compositions and methods.
Owner:PHOTOKINETIX HLDG
Compositions and methods for treatment of taupathy
Provided are electrokinetically-altered fluids (e.g., electrokinetically-altered gas-enriched fluids and solutions) comprising an ionic aqueous solution of charge-stabilized oxygen-containing nanostructures in an amount sufficient for treating an inflammatory neurodegenerative condition or disease (e.g., a taupathy) or at least one symptom thereof. The electrokinetically-altered fluids or therapeutic compositions and methods include electrokinetically-altered ionic aqueous fluids optionally in combination with other therapeutic agents. Particular aspects provide for modulating phosphorylation of tau protein. Particular aspects provide for regulating or modulating intracellular signal transduction associated with said inflammatory responses by modulation of at least one of cellular membrane potential and / or conductance, membrane proteins such as membrane receptors, including but not limited to G-Protein Coupled Receptors (GPCR), and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes). Other embodiments include particular routes of administration or formulations for the electrokinetically-altered fluids and therapeutic compositions.
Owner:REVALESIO CORP
Activation and monitoring of cellular transmembrane potentials
ActiveUS20120034622A1Different optical propertyLarge Stokes shiftCompound screeningApoptosis detectionBiological activationNanostructure
The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed.
Owner:LIFE TECH CORP
Alteration of cell membrane with B7
InactiveUS7238360B2Systemic effectInhibitionOrganic active ingredientsPeptide/protein ingredientsForeign proteinApoptosis
Methods and compositions are provided for the persistent modification of cell membranes with exogenous proteins so as to alter the function of the cell to achieve effects similar to those of gene therapy, without the introduction of exogenous DNA. DNA sequences, the proteins and polypeptides embodying these sequences are disclosed for modulating the immune system. The modulations include down-regulation, up-regulation and apoptosis.
Owner:UNIV OF LOUISVILLE RES FOUND INC
Struvite crystallization
The present invention provides a method and apparatus for removing phosphorus from phosphorus containing waste. In one embodiment, the method is preferably carried out by contacting the phosphorus containing waste with a non-cellular membrane and precipitating phosphorus from the waste as struvite. Another aspect of the invention includes a method of removing phosphorus from phosphorus containing sewage comprising filtrates and biosolids. The removal of phosphorus as struvite occurs in two stages as primary and secondary removal. In the primary removal process, the sewage from a dewatering unit is contacted with a first polymeric membrane reactor and the phosphorus is removed as primary struvite. Subsequently Mg is added so as promote struvite formation and the secondary removal process of struvite. In the secondary removal process, the sewage from GBT Filtrate well or Centrifuge Liquor well is contacted with a second monomolecular membrane and the phosphorus is removed as secondary struvite.
Owner:WISCONSIN ALUMNI RES FOUND
Disruption and field enabled delivery of compounds and compositions into cells
ActiveUS20180016539A1Other foreign material introduction processesElectrical/wave energy microorganism treatmentCell membraneMicrofluidic channel
A microfluidic system for causing perturbations in a cell membrane includes (a) a microfluidic channel defining a lumen and configured such that a cell suspended in a buffer can pass there through, and (b) source or emitter of an energy field. The microfluidic channel may include a cell-deforming constriction. A diameter of the constriction may be a function of the diameter of the cell. Related apparatus, systems, techniques, and articles are also described.
Owner:MASSACHUSETTS INST OF TECH
Electrokinetically-altered fluids comprising charge-stabilized gas-containing nanostructures
Provided are electrokinetically-altered fluids (e.g., gas-enriched (e.g., oxygen-enriched) electrokinetic fluids) comprising an ionic aqueous solution of charge-stabilized gas-containing nanostructures in an amount sufficient to provide, upon contact with a cell, modulation of at least one of cellular membrane potential and cellular membrane conductivity. Further provided are the methods of making the electrokinetically-altered ionic aqueous fluid compositions. Particular aspects provide for regulating or modulating intracellular signal transduction associated by modulation of at least one of cellular membranes, membrane potential, membrane proteins such as membrane receptors, including but not limited to G-Protein Coupled Receptors (GPCR), and intercellular junctions (e.g., tight junctions, gap junctions, zona adherins and desmasomes). Other embodiments include particular methods of producing the electrokinetically-altered fluids. The electrokinetically-altered fluid compositions and methods of producing the fluid include electrokinetically-altered ionic aqueous fluids optionally in the form of solvated electrons stabilized, at least in part, with molecular gas (e.g., oxygen).
Owner:REVALESIO CORP
Compositions and methods for treating asthma and other lung disorders
InactiveUS20120114702A1Sufficient amountAntibacterial agentsPowder deliveryObstructive Pulmonary DiseasesOxygen
Provided are compositions and methods for treating lung or respiratory disorders or conditions characterized by airflow obstruction or limitation, or symptoms thereof (e g, asthma, rhinitis, allergic rhinitis, and chronic obstructive pulmonary disease (CaPO) and CaPO-associated conditions (e g, bronchitis, emphysema, asthma), emphysema, pneumonia, bronchitis, in-fluenza, SARS, tuberculosis, and whooping cough (pertussis), and the like) comprising administering a therapeutic composition comprising at least one electrokinetically altered fluid comprising an ionic aqueous solution of charge-stabilized oxygen containing nanostructures as disclosed herein, or comprising administering a nonelectrokinetic superoxygenated aqueous solution The methods preferably comprise regulating intracellular signal transduction by modulation of at least one of cellular membranes, membrane potential, membrane proteins (e g, membrane receptors, (e g, G protein-coupled receptors, and intercellular junctions)) Additional aspects include therapeutic compositions, and combination treatment methods comprising administration of electrokinetically generated fluid in combination with at least one additional therapeutic agent (e g, albuterol, etc).
Owner:REVALESIO CORP
Lipid-free scaffolds for human volume replacement or cell culture and use thereof
ActiveUS20100178681A1Improve productivityQuality improvementTissue cultureElectrical/wave energy microorganism treatmentLipid formationVolume replacement
The present invention relates to lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture. More particularly, the present invention relates to a method for preparing lipid removed scaffolds(lipid-free scaffolds) for human tissue volume replacement or cell culture, the method comprising the steps of: fragmenting fat tissue to isolate lipids by ultrasonic treatment or high pressure nozzle spray; removing the isolated lipids and fat tissue from which lipids were not isolated to sterilize. According to the present invention, lipids are removed from fat tissue only by physical treatment to maintain the volume thereof and microstructures such as cellular membrane as much as possible and thus the inventive scaffolds are useful for human tissue volume replacement.
Owner:LEE HEEYOUNG
Fluorescent carbon quantum dots for monitoring pH of acid environment and preparation method and application of fluorescent carbon quantum dots
InactiveCN110172344AEasy to prepareGood water solubility and biocompatibilityNanoopticsNano-carbonChemistryQuantum yield
The invention provides fluorescent carbon quantum dots for monitoring pH of an acid environment and a preparation method and application of the fluorescent carbon quantum dots. The preparation methodcomprises the steps that (1) absolute ethyl alcohol is added into o-phenylenediamine and p-aminobenzene sulfonic acid, and after sufficient stirring, ultrasonic dissolution is conducted; (2) the abovemixture is transferred into a hydrothermal reaction kettle, and the hydrothermal reaction kettle is placed in a drying oven for a reaction for 8-10 hours at 180-200 DEG C; (3) after the reaction is stopped, standing, cooling to the indoor temperature, filtration, centrifugation and removal of undissolved substances are conducted, a blue-black solution is obtained, and after filtration by a filtering membrane, a pure carbon dot aqueous solution is obtained; (4) the above carbon dot aqueous solution is subjected to vacuum drying to obtain the target product. The method is simple, the requirements for preparation conditions are low, and pretreatment and a purification process are not needed. The prepared carbon quantum dots have the advantages of long wavelength transmission, a stable optical property, good biocompatibility, the high quantum yield and the like; the carbon quantum dots quite sensitively respond to the pH, can quickly penetrate through the cell membranes, enter cells and monitor fluctuation of the pH in living cells in real time, and are capable of being used for monitoring the pH of the acid environment, especially the pH of an extremely acid environment.
Owner:SHANXI UNIV
Method and apparatus for optical recording of biological parameters in freely moving animals
InactiveUS10292592B2Reduce low frequency noiseReduce crosstalkDiagnostics using fluorescence emissionSensorsOptical measurementsLength wave
A system and method for monitoring biological parameters in freely moving animals that, in the illustrative embodiment, comprises a two-color optical measurement / recording system that is combined with a fluorescent protein reporter of cellular membrane potentials and a fluorescent protein that is insensitive to such cellular membrane potentials. The two wavelengths are used to un-mix physiological artifacts in recordings that occur in freely moving animals.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Method and apparatus for optical recording of biological parameters in freely moving animals
InactiveUS20160135754A1Reduce low frequency noiseReduce crosstalkDianostics using fluorescence emissionSensorsCell membraneOptical measurements
A system and method for monitoring biological parameters in freely moving animals that, in the illustrative embodiment, comprises a two-color optical measurement / recording system that is combined with a fluorescent protein reporter of cellular membrane potentials and a fluorescent protein that is insensitive to such cellular membrane potentials. The two wavelengths are used to un-mix physiological artifacts in recordings that occur in freely moving animals.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Method and Device for Identifying Cells
ActiveUS20140284221A1Small footprintAccurate methodImmobilised enzymesBioreactor/fermenter combinationsCell membraneCell based
The present invention provides a method to analyze or identify a cell. The method comprises: providing a cell, stimulating the cell with a stimulant thereby modifying a cell membrane impedance of the cell, monitoring the cell membrane impedance of the cell and identifying the cell based on the monitored cell membrane impedance. A corresponding device is also provided.
Owner:INTERUNIVERSITAIR MICRO ELECTRONICS CENT (IMEC VZW)
Preparation method of nanopore cellular membrane
Owner:ZHEJIANG UNIV
System and method for electronic sensing of biomolecules
InactiveUS20100047901A1Reduces or eliminates non-specific bindingBioreactor/fermenter combinationsMaterial nanotechnologyEngineeringField-effect transistor
A nanoelectronic device is combined with a cellular membrane component to provide a sensor for biomolecules or to provide information about the structure of the membrane. The nanoelectronic device may comprise a network of randomly-oriented nanotubes, or other nanostructure, arranged on a substrate with adjacent electrodes so as to operate as a field-effect transistor sensor or as a capacitive sensor. A cellular membrane is disposed over the nanostructure element.
Owner:NANOMIX INC
Blood diagnosis method for dialysis patient and dialysis machine
InactiveUS20100112583A1Convenient treatmentMicrobiological testing/measurementWithdrawing sample devicesDiagnosis methodsBiomarker (petroleum)
Provided is a blood diagnosis method and a dialysis machine, using a diagnostic marker which is versatile and which can contribute to the improvements in dialysis treatment and the evaluation of clinical effects,the method including a step for collecting a blood sample from a dialysis patient before and after dialysis; and a step for making a diagnosis regarding the collected blood sample based on a biomarker, wherein the biomarker is identified in advance based on the correlation between the urea clear space (CS) or the cellular membrane clearance (Kc) and profiles of mRNA or proteins.
Owner:YOKOGAWA ELECTRIC CORP +1
System and method for optogenetic therapy
Configurations are described for utilizing light-activated proteins within cell membranes and subcellular regions to assist with medical treatment paradigms, such as hypertension treatment via anatomically specific and temporally precise modulation of renal plexus activity. The invention provides for proteins, nucleic acids, vectors and methods for genetically targeted expression of light-sensitive proteins to specific cells or defined cell populations. In particular the invention provides systems, devices, and methods for millisecond-timescale temporal control of certain cell activities using moderate light intensities, such as the generation or inhibition of electrical spikes in nerve cells and other excitable cells.
Owner:CIRCUIT THERAPEUTICS
Radio-frequency electrical membrane breakdown for the treatment of high risk and recurrent prostate cancer, unresectable pancreatic cancer, tumors of the breast, melanoma or other skin malignancies, sarcoma, soft tissue tumors, ductal carcinoma, neoplasia, and intra and extra luminal abnormal tissue
ActiveUS20180263685A1Safe and effectiveSurgical needlesVaccination/ovulation diagnosticsCellular componentAnesthesia needle
An imaging, guidance, planning and treatment system integrated into a single unit or assembly of components, and a method for using same, that can be safely and effectively deployed to treat prostate cancer in all medical settings, including in a physician's office or in an outpatient setting. The system utilizes the novel process of Radio-Frequency Electrical Membrane Breakdown (“EMB” or “RFEMB”) to destroy the cellular membranes of unwanted or cancerous tissue without denaturing the intra-cellular contents of the cells comprising the tissue, thereby exposing tumor antigens and other intra-cellular components which can have an immunologic effect on local or distant cancerous tissue, with or without the addition of immunologic adjutant drugs. The system preferably comprises at least one EMB treatment probe 20, at least one trackable biopsy needle 200, at least one trackable anesthesia needle 300, and at least one controller unit for at least partially automating the treatment process.
Owner:IMMUNSYS INC
Method and device for obtaining biophysical characteristics of ultra-diffraction limit cell membrane micro-structure
ActiveCN105572044AAchieve acquisitionMaterial analysis by optical meansScanning probe microscopyMicro structureSpatial light modulator
The invention relates to a method and device for obtaining the biophysical characteristics of an ultra-diffraction limit cell membrane micro-structure. The device comprises a laser device and a semiconductor laser device. The laser output path of the laser device sequentially comprises: a polarizer, a first convergent lens set, an aperture, a first reflector, a spatial light modulator, a third convergent lens, a light barrier, a second convergent lest set, and a dichroscope; the reflected light output path of the dichroscope sequentially comprises the objective lens and the objective table of an optical microscope; the transmission light output path of the dichroscope sequentially comprises a filter, a switchable reflector, a sixth convergent lens, and a CCD detector; the laser output path of the semiconductor laser device sequentially comprises a second reflector, a probe, which is driven by a three dimensional controller, and a position sensitive detector; and the CCD detector, the three dimensional controller, and the position sensitive detector are all connected to a data collecting and controlling system. The provided method and device can obtain the information of biophysical characteristics of an ultra-diffraction limit cell membrane micro-structure.
Owner:FUJIAN NORMAL UNIV
Nano cell membrane drug-loaded vesicle, preparation method and application thereof
InactiveCN111494341AExtension of timeGood treatment effectPeptide/protein ingredientsPharmaceutical non-active ingredientsLiver and kidneyCoated drugs
The invention discloses a nano cell membrane drug-loaded vesicle, a preparation method and application thereof. The preparation method is characterized in that through a physical extrusion technology,drug coating is carried out in the process of forming nanoscale cell membrane vesicles, no influence is generated on the activity of a coated drug, the in-vivo circulation time of the drug can be prolonged, the drug targeting ability is improved, and directional slow release of the drug is realized. The preparation method comprises the steps of cell membrane acquisition and cell membrane nano-vesicle preparation. Specific cell delivery of the drug targeting specific part can be realized, so that the drug fully plays a therapeutic role, and liver and kidney injury caused by one-time massive drug aggregation in the liver or kidney is prevented. Dynamic changes in a drug body can be tracked through probe wrapping.
Owner:NANKAI UNIV
Activation and monitoring of cellular transmembrane potentials
InactiveCN102119331AHigh quantum yieldEasy to understandCompound screeningNanostructure manufactureBiological activationNanostructure
Owner:LIFE TECH CORP
Multi-component antioxidant compounds, pharmaceutical compositions containing same and their use for reducing or preventing oxidative stress
Owner:YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
Monitoring and manipulating cellular transmembrane potentials using nanostructures
The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed. Nanoparticles having phospholipid coatings were found to display improved responses relative to nanoparticles having other coatings that do not promote localization or attraction to membranes.
Owner:LIFE TECH CORP
Daidzein derivative and preparation method thereof
InactiveCN101659648AChange polarityChange the spatial structureOrganic active ingredientsOrganic chemistrySolubilityDaidzein
The invention relates to a daidzein derivative which has the following chemical formula: in the chemical formula, at least one of R1 and R2 is carbonyl containing 1 to 3 carbon atoms, R3 and R4 are selected from alkyl, amino, amino derivative, ureido, guanidyl and amino acid containing 1 to 4 N atoms. The derivative adopts the daidzein as raw material, firstly carries out acylation with acylate reagent so as to obtain a midbody, and then obtains target product by reaction of midbody with R3 and / or R4. The derivative changes the polarity of the daidzein and space structure, improves water solubility, also improves permeability function of the cellular membrane and effectively improves the oral bioavailability thereof.
Owner:HEFEI UNIV OF TECH
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