Fluorescent carbon quantum dots for monitoring pH of acid environment and preparation method and application of fluorescent carbon quantum dots

A technology of carbon quantum dots and fluorescence, applied in the field of luminescent nanomaterials, can solve the problems of weak penetration ability of carbon dots, limited application, photodamage of biological tissues and side effects, and achieve good water solubility and biocompatibility, preparation method easy effect

Inactive Publication Date: 2019-08-27
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in most applications, the emission of various carbon dots is concentrated in the green and blue regions, and due to the weak penetrating ability of carbon dots, as well as the great photodamage and side effects of short-wave radiation on biological tissues, this greatly limits their application in biology

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  • Fluorescent carbon quantum dots for monitoring pH of acid environment and preparation method and application of fluorescent carbon quantum dots
  • Fluorescent carbon quantum dots for monitoring pH of acid environment and preparation method and application of fluorescent carbon quantum dots
  • Fluorescent carbon quantum dots for monitoring pH of acid environment and preparation method and application of fluorescent carbon quantum dots

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation method of carbon dots:

[0030] (1) Take 0.108g of o-phenylenediamine and 0.173g of p-aminobenzenesulfonic acid respectively in a container, add 20mL of absolute ethanol, stir thoroughly and ultrasonically dissolve for 20 minutes;

[0031] (2) Transfer the above to a hydrothermal reaction kettle, place it in an oven, and react at 200°C for 8 hours;

[0032] (3) After the reaction stops, let it stand and cool to room temperature, filter, centrifuge at 8000r / m for 15min, remove insoluble matter to obtain a dark blue solution, and filter with a 0.22 μm filter membrane to obtain a pure aqueous solution of carbon dots;

[0033] (4) The target product was obtained after the above-mentioned carbon dot aqueous solution was vacuum-dried, and its relative quantum yield (based on quinine sulfate) was 15.97%;

[0034] The obtained carbon quantum dots were respectively dissolved in PBS buffer solutions of pH=7.0, pH=3.0 and pH=1.5 to form a solution of 0.4 mg / ml, which ...

Embodiment 2

[0041] (1) Take 0.108g of o-phenylenediamine and 0.087g of p-aminobenzenesulfonic acid respectively in a container, add 20mL of absolute ethanol, stir thoroughly and ultrasonically dissolve for 20 minutes;

[0042] (2) Transfer the above to a hydrothermal reaction kettle, place it in an oven, and react at 200°C for 8 hours;

[0043] (3) After the reaction stops, let it stand and cool to room temperature, filter, centrifuge at 8000r / m for 15min, remove insoluble matter to obtain a dark blue solution, and filter with a 0.22 μm filter membrane to obtain a pure aqueous solution of carbon dots;

[0044] (4) The target product was obtained after vacuum-drying the above carbon dot aqueous solution, and its relative quantum yield (based on quinine sulfate) was 9.93%.

Embodiment 3

[0046](1) Take 0.108g of o-phenylenediamine and 0.087g of p-aminobenzenesulfonic acid respectively in a container, add 10mL of absolute ethanol, stir thoroughly and ultrasonically dissolve for 20 minutes;

[0047] (2) Transfer the above to a hydrothermal reaction kettle, and place it in an oven, and react at 180°C for 10 hours;

[0048] (3) After the reaction stops, let it stand and cool to room temperature, filter, centrifuge at 8000r / m for 15min, remove insoluble matter to obtain a dark blue solution, and filter with a 0.22 μm filter membrane to obtain a pure aqueous solution of carbon dots;

[0049] (4) The target product was obtained after vacuum-drying the above carbon dot aqueous solution, and its relative quantum yield (based on quinine sulfate) was 8.08%.

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Abstract

The invention provides fluorescent carbon quantum dots for monitoring pH of an acid environment and a preparation method and application of the fluorescent carbon quantum dots. The preparation methodcomprises the steps that (1) absolute ethyl alcohol is added into o-phenylenediamine and p-aminobenzene sulfonic acid, and after sufficient stirring, ultrasonic dissolution is conducted; (2) the abovemixture is transferred into a hydrothermal reaction kettle, and the hydrothermal reaction kettle is placed in a drying oven for a reaction for 8-10 hours at 180-200 DEG C; (3) after the reaction is stopped, standing, cooling to the indoor temperature, filtration, centrifugation and removal of undissolved substances are conducted, a blue-black solution is obtained, and after filtration by a filtering membrane, a pure carbon dot aqueous solution is obtained; (4) the above carbon dot aqueous solution is subjected to vacuum drying to obtain the target product. The method is simple, the requirements for preparation conditions are low, and pretreatment and a purification process are not needed. The prepared carbon quantum dots have the advantages of long wavelength transmission, a stable optical property, good biocompatibility, the high quantum yield and the like; the carbon quantum dots quite sensitively respond to the pH, can quickly penetrate through the cell membranes, enter cells and monitor fluctuation of the pH in living cells in real time, and are capable of being used for monitoring the pH of the acid environment, especially the pH of an extremely acid environment.

Description

technical field [0001] The invention relates to a luminescent nanometer material, in particular to a fluorescent carbon quantum dot, in particular to a fluorescent carbon quantum dot for monitoring the pH of an acidic environment, a preparation method and application thereof. Background technique [0002] Hydrogen ions are one of the most important intracellular species. Cell survival mainly depends on maintaining a balanced pH, regulating many metabolic pathways, such as cell growth and apoptosis, signaling and defense, etc. Small changes in intracellular pH can indicate abnormal cellular events and cause cellular dysfunction as well as cardiorespiratory and neurological diseases such as cancer and Alzheimer's disease. Maintaining pH homeostasis is fundamental to all living organisms. Intracellular pH changes and distribution measurements are of great significance for better understanding of cellular functions and provide critical aids for early disease diagnosis. Theref...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65C01B32/15G01N21/6428
Inventor 郭忠慧杜芳芳焦媛双少敏董川王煜
Owner SHANXI UNIV
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