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Method for forming parthenogenetic blastocyst through continuous vibration stimulation of human immature oocyte and vibration device

An oocyte, continuous vibration technology, applied in the field of developmental biology, to achieve the effect of convenient use and simple structure of the device

Active Publication Date: 2022-03-25
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology allows people who are unable to produce eggs due to gene defects that affect their ability to develop into healthy children when they have no natural egg production (parenchymal) capacity. It uses gentle movements from an artificially produced ovum called pluripotency to encourage this process. By continuously shaking these tiny cloned primordial germ cell cultures around them, it becomes possible to create new forms of life like humans' own fetuses.

Problems solved by technology

The technical problem addressed in this patented text relates to how certain types of stem or somatic tissue (parallelogram) may develop into cancerous tumors during chemotherapy treatments for diseases such as leukemia.

Method used

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  • Method for forming parthenogenetic blastocyst through continuous vibration stimulation of human immature oocyte and vibration device
  • Method for forming parthenogenetic blastocyst through continuous vibration stimulation of human immature oocyte and vibration device

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Embodiment 1

[0026] A method for forming a parthenogenetic blastocyst by continuous vibration stimulation of human immature oocytes, the steps of the method are as follows:

[0027] (1) In vitro maturation: human immature oocytes that are about to be discarded after egg retrieval are transferred to culture dish Ⅰ containing in vitro maturation culture medium after the patient’s informed consent, and culture dish Ⅰ is placed on a vibrating device. The vibrating device was placed in 37°C, CO 2 In an incubator with a volume fraction of 6%, culture was continuously vibrated at 10 Hz for 24 hours, and mature oocytes were obtained in Petri dish Ⅰ.

[0028] (2) Parthenogenetic activation: transfer the mature oocytes into the culture dish II containing the activation medium, place the culture dish II on the vibrating device in the incubator, vibrate continuously for 5 minutes at 10 Hz, and then transfer Put it into the petri dish III containing 6-dimethylaminopurine, and culture it with continuou...

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Abstract

The invention relates to a method for forming parthenogenetic blastocysts through continuous vibration stimulation of human immature oocytes and a vibration device, and belongs to the technical field of developmental biology. According to the method, human immature oocytes are subjected to in-vitro maturation, parthenogenetic activation and parthenogenetic blastocyst culture under continuous vibration stimulation, and finally parthenogenetic blastocysts are obtained. According to the method, the maturation of human immature oocytes and the development potential of embryos are promoted by adopting dynamic culture. According to the device, the vibration driving mechanism simply drives the culture dish to vibrate up and down in the direction perpendicular to the top of the box body, so that continuous vibration culture of cells is realized.

Description

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Claims

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Application Information

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Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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