Oocyte in-vitro maturation culture solution additive and application thereof

Abstract

The invention discloses an oocyte in-vitro maturation culture solution additive. The in-vitro maturation culture solution additive contains ceruloplasmin. Therefore, a first polar body excretion rateof oocytes can be significantly increased, the ROS content of the oocytes can be significantly reduced, and the embryonic blastocyst rate after parthenogenetic activation can be significantly increased, so that the maturation rate and quality of the oocytes can be greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an oocyte maturation culture medium additive in vitro and its application. Background technique [0002] Mammalian in vitro fertilized embryos, cloned embryos, parthenogenetic embryos and transgenic embryos are widely used in the field of embryo engineering technology such as genetic improvement, early embryo development mechanism research, epigenetic regulation, etc. The quality of oocyte culture in vitro is the key to determining embryo development However, so far the maturation efficiency and quality of oocytes cultured in vitro have not been greatly improved. Reactive oxygen species (reactive oxygen species, ROS) is an important factor affecting oocyte culture in vitro. ROS is a product of biological aerobic metabolism, including oxygen ions, superoxide ions, hydroxyl radicals, and hydrogen peroxide. In equilibrium, ROS, as signaling molecules, play beneficial roles in physiolo...

AI Technical Summary

Problems solved by technology

However, in vitro culture is a static environment without the replacement of nutrients and metabolites, which tends to cause the accumulation of ROS, which alters their function and impairs cell survival.

Thus, ROS negatively impact oocyte viability, gene expression, protein synthesis, and molecular signaling, affecting oocyte maturation and developmental competence

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