53results about How to "Increase blastocyst rate" patented technology

The invention provides a method for delaying the aging of eggs cultured in vitro, which belongs to the technical field of animal reproduction. The present invention finds that adding astaxanthin (Astaxanthin) during in vitro culture of eggs in culture solution can effectively improve the early embryonic development of aging eggs, increase the membrane potential of aging eggs, and reduce the apoptosis rate of aging eggs. The method of adding astaxanthin in the process of culturing eggs in vitro significantly slows down the aging speed of eggs cultured in vitro, improves the early embryonic development rate of aging eggs, and improves the development rate of aging eggs compared with conventional culture medium. 15%, able to provide high-quality eggs and early embryos for in vitro fertilization-embryo transfer (IVF-ET) technology.

The invention provides sheep embryonic cell culture fluid. The sheep embryonic cell culture fluid comprises the following components: calcium chloride dehydrate, anhydrous magnesium sulfate, potassium chloride, monopotassium phosphate, sodium bicarbonate, sodium chloride, bovine serum albumin, glucose, sodium pyruvate, sodium lactate and phenol red sodium salt. Embryonic fluid at a volume by concentration of 10% is also added to the culture fluid so that the culture fluid has a great cell culture effect. According to the culture fluid, the components collaborate with each other, so that the irritation to an early embryo is reduced, and the embryo can grow well; and in addition, 10% of uterine embryonic fluid of a ewe is added to the culture fluid, so that the fusion rate of a reconstructed embryo can be remarkably increased. Compared with the culture fluid without the embryonic fluid, the culture with the embryonic fluid has the capability of obviously increasing the survival rate and blastocyst rate of in-vitro cultured embryos; and the culture fluid provided by the invention also can improve uterine receptivity, therefore, the lambing percentage is increased obviously.

The invention relates to the technical field of buffalo embryo development, in particular to a method for improving the developmental rate of river-type buffalo in vitro fertilized embryos. %), it can still achieve the effect of increasing the division rate and blastocyst rate of buffalo fertilized eggs, thereby improving the buffalo in vitro embryo development rate and production efficiency; in the formula, in order to activate the activity of oocytes, the applicant has done a lot of research It was found that the use of cysteine, methionine, alanine, lysine, tryptophan and glycine dissolved in TCM199 solution and then mixed with follicular fluid for chelation can significantly improve the effect of follicular fluid on the promotion of embryonic development rate.

The invention belongs to the technical field of biological engineering and discloses a preparation method of pig cloning embryos. The preparation method comprises the following steps of: obtaining donor cells; taking recombinant vectors of reprogramming factors, transferring into the donor cells and obtaining nuclear donor cells; transplanting the nuclear donor cells into egg cells with removed cell nucleuses, and carrying out fusion and activation to obtain the pig cloning embryos. The preparation method provided by the invention has the advantages that the nuclear donor cells are directly transplanted into egg cells with removed cell nucleuses after being obtained, the step of utilizing the reprogramming factors to prepare the pig cloning embryos is reduced, the operation is simple and easy, the working period is shortened and the growing efficiency of the pig cloning embryos is effectively improved.

The invention discloses a porcine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof and belongs to the technical field of oocyte in-vitro maturation culture. In order to solve the problems of low porcine oocyte in-vitro maturation rate and development rate at present, the invention provides the porcine oocyte in-vitro maturation culture solution aswell as the preparation method and application thereof. The culture solution comprises a base culture solution TCM-199, penicillin, streptomycin, NaHCO3, 4-hydroxyethylpiperazine ethane sulfonic acid, polyvinyl alcohol, sodium pyruvate, insulin, cysteine, an epidermal growth factor, a porcine follicular fluid, pregnant mare serum gonadotropin, human chorionic gonadotropin and ramelteon. The culture solution can increase the oocyte in-vitro maturation quality, the cumulus cell diffusion index, the glutathione level, the parthenogenetic embryo blastocyst rate and blastocyst total cell number, the in-vitro fertilization embryo cleavage rate, the blastocyst rate and blastocyst total cell number and decrease the ROS level of oocytes.