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Mouse sperm in-vitro culture kit capable of improving blastocyst rate and using method thereof

A technology for in vitro culture and sperm culture solution, applied in the field of in vitro culture kits, can solve the problems of insufficient improvement of sperm motility, and the ratio needs to be improved, so as to achieve the effects of improving sperm motility and blastocyst rate and reducing preparation cost.

Active Publication Date: 2019-01-04
SUZHOU MUNICIPAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But two outstanding problems are: 1. The improvement of sperm motility is not enough; 2. The ratio of fertilized zygotes to develop into blastocyst stage (blastocyst rate) needs to be improved

Method used

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  • Mouse sperm in-vitro culture kit capable of improving blastocyst rate and using method thereof
  • Mouse sperm in-vitro culture kit capable of improving blastocyst rate and using method thereof

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the drawings.

[0026] Mouse sperm in vitro culture kit to increase blastocyst rate, including box body, 10mL of mHTF (Modified Human Tubal Fluid, modified human fallopian tube fluid), 100mg of BSA (bovine serum albumin, Bovine Serum Album), 5ug of ERO1L recombinant protein And 10ug of DPEP2 recombinant protein. mHTF, BSA, ERO1L recombinant protein, and DPEP2 recombinant protein are respectively stored in four discrete cavities in the box.

[0027] The ERO1L recombinant protein is an endoplasmic reticulumoxidoreductase 1like protein (ie endoplasmic reticulumoxidoreductase 1like), and its gene ID in the DNA sequence database established by the National Center for Biotechnology Information (NCBI, National Center for Biotechnology Information) is 50527.

[0028] The DPEP2 recombinant protein is dipeptidase-2 (ie dipeptidase 2), and its gene ID in the DNA sequence database established by the National Center...

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Abstract

The invention discloses a mouse sperm in-vitro culture kit capable of improving a blastocyst rate and a using method thereof. A current blastocyst rate of mouse in-vitro fertilization is lower, and itis not beneficial to development of assisted reproductive technology research depending on a mouse embryo. The mouse sperm in-vitro culture kit capable of improving the blastocyst rate comprises a mLof mHTF, b mL of BSA, c mg of an ERO1L recombinant protein and d mg of a DPEP2 recombinant protein. a:b:c:d=2:20:1:2. The ERO1L recombinant protein is an endoplasmic reticulum oxidordeuctase 1 albuminoid, and a gene ID in a DNA sequence database is 319446. The ERO1L recombinant protein and the DPEP2 recombinant protein are added to the kit, so the kit is capable of remarkably improving sperm activity and the blastocyst rate.

Description

Technical field [0001] The invention belongs to the technical field of culture kits, and specifically relates to an in vitro culture kit for improving sperm viability and blastocyst rate of mice and a method of use thereof. Background technique [0002] Fertilization is the process by which sperm and egg combine to form a zygote (zygote), which marks the beginning of new life. In this process, the sperm and egg carriers are the genetic material from the father and the mother respectively. The quality of the sperm and egg determines the success or failure of early embryo development. At present, most of the cognition of the mysterious biological event of fertilization comes from the research of in vitro fertilization. However, so far, human understanding of fertilization has not been exhausted, and it is necessary to rely on in vitro fertilization technology to further study the mechanism of fertilization. [0003] The mouse model is currently an important method for in vitro fert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073C12N5/076
CPCC12N5/0604C12N5/061C12N2501/734C12N2501/71C12N2501/998
Inventor 郑波于骏李红吴亦波沈聪陈霞杨慎敏
Owner SUZHOU MUNICIPAL HOSPITAL
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