35results about How to "No toxic effects" patented technology

The invention discloses a marine antifouling nano paint, which is prepared from the following raw materials: epoxy phenolic resin, titanium dioxide, a nano biological antifouling agent, filler, an acetic acid, and glyceryl tristearate. Capsaicin in the nano-scale biological antifouling agent has a good approach-avoidance effect on marine organisms; tannic acid has an adhesion inhibition effect on barnacle and also has a paralysis function on the beetle; shogaol has strong ability of preventing adhesive ability of mussel. P-cinnamic acid sulfate has a strong adhesion inhibition effect on marine bacteria and barnacle, homarine can prevent adhesion of marine benthic diatom and barnacle, and diterpenes formamide has an effect of inhibiting the barnacle and shellfish larvae. The paint disclosed by the invention comprises a nano-scale pure natural biological antifouling agent, has the approach-avoidance effect on marine organisms, and destroys the adhesive ability of the marine organisms, so as to achieve the target of preventing and treating pollution. The marine antifouling nano paint does not generate any deleterious effect on the marine environment, is free of an injury to the marine organisms, and is purely natural and harmless.

The invention provides fusion protein 210 and applications thereof in virus replication optimization. The fusion protein 210 is a truncated part of P60 protein, preserves a function for promoting replication of a plurality of viruses of the P60, and does not generate toxicity on cells in an effective concentration range. The fusion protein 210 can increase the virus titer by two orders of magnitudes in a wide-spectrum range (0.0001-0.1 MOI), and therefore virus identification and detection can be facilitated, rhabdovirus VSV, flaviviridae HCV, parvoviridae EV71, and the like can be detected, and time for detection is shortened by 50% or less. The fusion protein 210 obtained by a prokaryotic expression system is high in expression efficiency and easy to purify, the one-step purification efficiency can be 85% or above, and the protein final concentration can be 3 mg / mL. Accordingly, through directly adding the fusion protein 210 into a virus culture medium, a plurality of virus isolate strains can be detected under low titer conditions, and operation is simple, convenient and rapid. The fusion protein 210 has important influences and significance in the fields of production, learning, researching, and the like of the virology.

The invention relates to a bone scaffold which is used for minimally invasive treatment of cancellous bone fractures of human beings and other animals, such as vertebral compression fracture, calcaneal fracture, tibial plateau collapse fracture and the like. The bone scaffold comprises a scaffold component I and a scaffold component II, wherein a dovetail tenon or a dovetail mortise on the inner side surface of the scaffold component II can slide, are completely inserted into the paired dovetail mortise or the dovetail tenon on the inner side surface of the scaffold component I, and are locked by a locking device, thereby completing the tenon-mortise joint and forming a whole; and the bone scaffold can play the roles of resetting, supporting and fixing in cancellous bone. An intermediate scaffold component can be arranged between the scaffold component I and the scaffold component II, the dovetail tenon or the dovetail mortise on the upper and the lower surface of the intermediate scaffold component can slide simultaneously, are completely inserted into the paired dovetail mortise or the dovetail tenon on the inner side surfaces of the scaffold component I and the scaffold component II, and are locked by the locking device, thereby completing the tenon-mortise joint and forming the whole. Each scaffold component is in the shape of a cuboid or a polygonal body. The transverse sections of the dovetail tenons and the dovetail mortises are shaped like dovetails, arcs or steps.

The invention discloses a mouse sperm in vitro culture kit and a mouse fertilization method for improving the blastocyst rate. Currently, the blastocyst rate of mouse in vitro fertilization is low, which is not conducive to the development of assisted reproductive technology research based on mouse embryos. The mouse sperm in vitro culture kit for improving blastocyst rate of the present invention comprises a milliliter of mHTF, b milligram of BSA, c microgram of ERO1L recombinant protein and d microgram of DPEP2 recombinant protein. a:b:c:d=2:20:1:2. The ERO1L recombinant protein is an endoplasmic reticulum oxidoreductase 1-like protein, and its gene ID in the DNA sequence database is 50527. The DPEP2 recombinant protein is dipeptidase-2, and its gene ID in the DNA sequence database is 319446. In the present invention, ERO1L recombinant protein and DPEP2 recombinant protein are added in the kit, so that the present invention can significantly improve sperm motility and blastocyst rate.