Living cell vitrification freezing fluid and freezing thawing method

A technology of vitrification and freezing liquid, which is applied in the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of low blastocyst rate and low survival rate, so as to improve the survival rate and the development rate of in vitro fertilization Effect

Inactive Publication Date: 2019-05-31
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low survival rate and low blastocyst rate in the prior art, the present invention provides a living cell vitrification freezing solution, which can improve the survival rate of living cells after freezing and thawing, the development rate of in vitro fertilization, egg Crack rate and blastocyst rate

Method used

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  • Living cell vitrification freezing fluid and freezing thawing method
  • Living cell vitrification freezing fluid and freezing thawing method
  • Living cell vitrification freezing fluid and freezing thawing method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A vitrification solution for living cells, which is based on MEM medium and F12 medium with a volume ratio of 1:1, adding 50nmol / L isopyridine, 20nmol / L chlorogenic acid and 40μmol / L A protective agent for L; the protective agent is adenosine triphosphate.

Embodiment 2

[0036] A kind of living cell vitrification liquid, this freezing liquid is based on the volume ratio of 1:1 MEM medium and F12 medium, add 55nmol / L of isoxapyridine, 25nmol / L of chlorogenic acid and 44μmol / L A protective agent for L; the protective agent includes 22 μmol / L tea polyphenols and 22 μmol / L lentinan.

Embodiment 3

[0038] A kind of living cell vitrification liquid, this freezing liquid is based on the MEM medium and F12 medium with a volume ratio of 1:1, adding 60nmol / L of isoxapyridine, 30nmol / L of chlorogenic acid and 48μmol / L Protective agent for L; said protective agent includes 12 μmol / L hydroxyethyl starch, 10 μmol / L tea polyphenols, 10 μmol / L 4-hydroxyethylpiperazineethanesulfonic acid, 8 μmol / L lentinan and 8 μmol / L adenine nucleoside triphosphates.

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Abstract

The invention provides a living cell vitrification freezing fluid and a freezing thawing method. The freezing fluid is prepared by that on the basis of an MEM culture medium and an F12 culture mediumin a volume ratio of 1:1, 50-60nmol / L of isofraxidin, 20-30nmol / L of chlorogenic acid and 40-48Mumol / L of a protective agent are added, wherein the protective agent comprises one or more of hydroxyethyl starch, tea polyphenol, 4-hydroxyethyl piperazine ethanesulfonic acid, lentinan and adenosine triphosphate. The living cell vitrification freezing fluid has the advantage that the living cell survival rate, the in-vitro fertilization and development rate, the cleavage rate and the blastocyst rate can be increased.

Description

technical field [0001] The invention relates to the technical field of vitrification, in particular to a living cell vitrification solution and a freezing recovery method. Background technique [0002] Living cells include but are not limited to egg cells, early embryos, blastocysts, etc. Among them, the freezing value of eggs is huge, and has important research and application value in the fields of rare animals, experimental animal breeding and regenerative medicine research. However, the current egg freezing technology Immature, the use of current freezing technology has disadvantages such as low egg survival rate after recovery, low rate of developing into blastocysts, and increased miscarriage rate. [0003] In some cases, only one usable egg cell can be obtained per operation. Therefore, cryogenic fluid, egg cell cryopreservation and resuscitation techniques play a crucial role in the success rate of transplantation. Contents of the invention [0004] In order to so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 宋莉莉董明珠刘世红潘新李帅民
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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