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Test evaluation method for sensitization of traditional Chinese medicine injection

An evaluation method and injection technology, applied in the field of detection, can solve the problems of ineffective detection of sensitization of traditional Chinese medicine injections, and achieve the effects of strong operational reliability, good experimental stability, and sensitive detection

Inactive Publication Date: 2014-03-05
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the technical problem to be solved by the present invention is to overcome the deficiency that the prior art cannot effectively detect the sensitization in traditional Chinese medicine injection, and provide a kind of traditional Chinese medicine injection with wide application range, high efficiency, stability, convenient and fast operation, and accurate results Sensitization and irritation testing methods

Method used

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  • Test evaluation method for sensitization of traditional Chinese medicine injection
  • Test evaluation method for sensitization of traditional Chinese medicine injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Allergenicity Detection of Reduning Injection

[0020] 1. Method

[0021] 1. Sensitive parts of ultrafiltration enriched traditional Chinese medicine injection

[0022] Take respectively 100ml of Reduning injection (batch numbers: 100808, 111011, 101105, 101114) with known adverse reactions and Reduning injection (batch numbers: 110709, 120207, 120210, 120211) of the sample to be tested, and add Transfer to an ultrafiltration tube with a molecular weight cut-off of 3KD, centrifuge at 3500 rpm for 40 minutes, and after repeated ultrafiltration, the remaining 5ml solution is obtained from the 20-fold concentrated and enriched macromolecular allergenic sites of the above batch numbers, sterilized, and stored at 4 degrees.

[0023] 2. Co-culture of splenic mononuclear cell PBMC and stimulatory site

[0024] Take the ICR mouse and kill it by decapitation, soak it in 75% alcohol for 2 minutes, take it out, open the abdominal cavity, take out the spleen, put it in ...

Embodiment 2

[0038] Example 2 Sensitivity Detection of Salvia Miltiorrhiza, Radix Astragali, Shengmai, Puerarin, Xuesaitong Injection

[0039] 1. Method

[0040] 1. Sensitive parts of ultrafiltration enriched traditional Chinese medicine injection

[0041] Take 100ml each of Danshen, Astragalus, Shengmai, Puerarin, and Xuesaitong injections, add them to an ultrafiltration tube with a molecular weight cut-off of 3KD, and centrifuge at 3500 rpm for 40min. After repeated ultrafiltration, 20 and 60 times concentrated and enriched large Molecular sensitization parts, sterilized, stored at 4 degrees, and set aside.

[0042]2. Co-culture of splenic mononuclear cell PBMC and stimulatory site

[0043] Take the ICR mouse and kill it by decapitation, soak it in 75% alcohol for 2 minutes, take it out, open the abdominal cavity, take out the spleen, put it in a petri dish, cut it into pieces, squeeze it with a needle core to disperse it, and blow it with PBS (5ml) to disperse it into cells The suspe...

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Abstract

The invention provides a test evaluation method for sensitization of a traditional Chinese medicine injection. The method comprises the steps of: treating a traditional Chinese medicine injection by ultrafiltration enrichment with 1-3KD to prepare a sensitized large molecular part; then adding the large molecular part into a peripheral blood mononuclear cell (PBMC) solution; carrying out co-culture for 1-3 days; and employing optimized trypan blue or fluorescent dye to dye living cell numbers, in order to calculate and evaluate sensitization strength of the traditional Chinese medicine injection. The method has advantages of convenience, rapidness and sensitivity, can be widely used in test and evaluation on sensitization and irritation of various traditional Chinese medicine injections, and provides scientific basis for safety evaluation and quality control of traditional Chinese medicine injections, and safety of clinical medication.

Description

technical field [0001] The invention relates to a detection method, specifically designing a detection and evaluation method widely applicable to the sensitization and irritation of traditional Chinese medicine injections. Background technique [0002] Traditional Chinese medicine injection is a modern Chinese medicine preparation developed in the modernization of Chinese medicine. It has remarkable clinical efficacy and is widely used in respiratory, cardiovascular and cerebrovascular fields, and has achieved good market economic benefits. However, traditional Chinese medicine injections often have adverse reactions in clinical use, which has potential safety hazards. Adverse reactions have been reported for commonly used Chinese medicine injections in clinical practice, such as Shuanghuanglian, Qingkailing, Radix Isatidis, Houttuynia cordata, Chuanhuning, Mailuoning, Puerarin, and Compound Danshen. [0003] There are various types of adverse reactions, the most common bei...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 马宏跃段金廒詹瑧萧伟王团结
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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