Full-automatic cell non-staining image recognizing and counting method

Abstract

The invention discloses a full-automatic cell non-staining image recognizing and counting method. The full-automatic cell non-staining image recognizing and counting method comprises the following steps of: selecting a full-automatic cell counting meter through an image recognition method, adding a cell suspension to be detected into a counting sheet directly, and inserting the cell suspension to be detected into a counting meter; lowering the receiving capability of a light sensing chip to the light intensity of a red light R and a green light G, intensifying the receiving capability of the light sensing chip to the light intensity of a blue light B, and regulating the aeration time and gain manually so as to change the background of a display to be light blue, and take round hot spots as images of active cells, and take the round blue spots darker than the background color as images of dead cells; and analyzing through cell image recognition software, thereby obtaining accurate large-small cell activity analysis data. According to the full-automatic cell non-staining image recognizing and counting method, dyes are not required to be used for counting cell activities; latent toxicity brought to cells to be detected by trypan blue is prevented; detected cells can continue to live, and the activities of the cells are counted during the culturing process; and meanwhile, according to the full-automatic cell non-staining image recognizing and counting method provided by the invention, non-staining real-time on-line cell counting can be achieved, and the method can be widely applied to the field of life science research.

Description

technical field [0001] The invention relates to a detection method, in particular to a fully automatic cell non-staining image recognition and counting method for detecting cell quantity and activity. Background technique [0002] Since the cells must maintain a certain concentration in order to have better activity during culture, the cells need to be counted several times according to the actual situation in the process of cell recovery, culture, and cryopreservation; at present, the commonly used cell counting methods include automatic image recognition method and electrical impedance counting method. [0003] The electrical impedance counting method is based on the detection of the resistance change caused by the cells suspended in the electrolyte solution when they pass through the counting channel, and the cell count and volume are determined. This method is called the electrical impedance method, also known as the Coulter principle. . [0004] Disadvantages of the e...

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Problems solved by technology

[0007] In order to solve the problems of the traditional cell counting method of image recognition after staining, the toxicity to living cells, the inability to count online, and the inability to stain embryonic cells For subsequent culture and proliferation and the inability to judge the activity and quantity of cells with endocytic function, the purpose of the present invention is to provide a fully automatic cell non-stained image recognition and counting method, which avoids the potential toxicity caused by the use of trypan blue, It can not only count the activity of cells of various sizes, but also can count the activity of cells that cannot be stained with trypan blue, such as germ cells and embryonic cells, and can also conveniently achieve accurate online counting

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