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Application of 450-490 nm LED-blue-light combined with arsenic trioxide in osteosarcoma treatment

A technology of osteosarcoma and osteosarcoma cells, applied in the medical field, can solve problems such as organ system lesions, achieve the effects of reducing side effects, significant effects, and reducing physical and mental damage

Inactive Publication Date: 2018-02-27
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But As 2 o 3 The treatment is a double-edged sword, it will produce severe stress response, and it will also cause systemic lesions in many important organs such as the heart and liver

Method used

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  • Application of 450-490 nm LED-blue-light combined with arsenic trioxide in osteosarcoma treatment
  • Application of 450-490 nm LED-blue-light combined with arsenic trioxide in osteosarcoma treatment
  • Application of 450-490 nm LED-blue-light combined with arsenic trioxide in osteosarcoma treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: As 2 o 3 Inhibit the growth and proliferation of osteosarcoma cells

[0028] 1 Experimental method

[0029] 1.1 Trypan blue staining to detect the survival rate of osteosarcoma cells

[0030] 1.2 Experimental grouping design:

[0031] According to As2 o 3 The different treatment concentrations were divided into the following five groups: 0 μM; 0.1 μM; 0.3 μM; 1 μM; 3 μM and 10 μM.

[0032] 1.3 Experimental testing items: trypan blue staining, cell counting

[0033] In U2OS osteosarcoma cells, 0 μM, 0.1 μM, 0.3 μM, 1 μM, 3 μM and 10 μM As were applied 2 o 3 After 24 hours of treatment, stain with trypan blue and count the cells.

[0034] 1.4 Data processing

[0035] The results of the present invention are represented by standard deviation±standard error. Graphpad prism 5.0 was used for graphing, and the correlation between each group was measured by T test.

[0036] 2 Observations

[0037] Such as figure 1 As shown, compared with the 0μM group, t...

Embodiment 2

[0038] Example 2 0.3 μM As 2 o 3 Combined with 450-490nm LED-blue light to inhibit the growth and proliferation of osteosarcoma cells

[0039] 1 Experimental method

[0040] 1.1 Trypan blue staining and EdU staining to detect the survival rate of osteosarcoma cells

[0041] 1.2 Experimental grouping design:

[0042] The experiment was divided into 4 groups: control group, 0.3μM As 2 o 3 Treatment group, LED-blue light 180J / cm 2 group (illumination power is 100mW / cm 2 , the light time is 30min, the total light intensity is 180J / cm 2 ) and combined application group, the combined application group selected 0.3μM As 2 o 3 The osteosarcoma cells were treated for 2 hours, and the light experiment was carried out by LED-blue light source with a wavelength of 450-490nm, and the light power was 100mW / cm 2 The light time is 30min, and the total light intensity is 180J / cm 2 .

[0043] 1.3 Experimental testing items: trypan blue staining, cell counting and EdU staining

[00...

Embodiment 3

[0049] Example 3: 0.3 μM As 2 o 3 Combined with 450-490nm LED-blue light to inhibit U2OS cell migration

[0050] 1 Experimental method

[0051] 1.1 Scratch test to detect the migration ability of osteosarcoma cells

[0052] 1.2 Experimental grouping design:

[0053] The experiment was divided into 4 groups: control group, 0.3μM As 2 o 3 Treatment group, LED-blue light 180J / cm 2 group (illumination power is 100mW / cm 2 , the light time is 30min, the total light intensity is 180J / cm 2 ) and combined application group, the combined application group selected 0.3μM As 2 o 3 The osteosarcoma cells were treated for 2 hours, and the light experiment was carried out by LED-blue light source with a wavelength of 450-490nm, and the light power was 100mW / cm 2 The light time is 30min, and the total light intensity is 180J / cm 2 .

[0054] 1.3 Scratch test

[0055] Scratch the surface of U2OS osteosarcoma cells with a 200 μl pipette tip to keep the width of the scratch as consis...

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Abstract

The invention discloses application of 450-490 nm LED-blue-light combined with arsenic trioxide in osteosarcoma treatment. Trypan-blue dyeing, cell counting and the EdU dyeing method are adopted to detect the influence of the 450-490 nm LED-blue-light combined with the arsenic trioxide on osteosarcoma cell growth and proliferation, scratch tests and Transwell tests are applied to detecting the influence on cell migration and invasion capabilities, and gamma-H2AX immunofluorescent staining is applied to detecting the influence on cell nucleus damage. Test results show that through combination of the 450-490 nm LED-blue-light and As2O3, curative effects of independently used small-dosage As2O3 can be improved, the toxicity is reduced, the growth, proliferation, migration and invasion of osteosarcoma cells can be effectively inhibited, the cell nucleus damage is induced, and then the purposes of relieving and treating osteosarcoma are achieved. The clinical application of the 450-490 nm LED-blue-light combined with arsenic trioxide is simple and low in medical treatment cost, pain of treated patients is reduced at the same time, and a new technological means is provided for the osteosarcoma treatment.

Description

technical field [0001] The invention relates to 450-490nm LED-blue light combined with arsenic trioxide (Arsenic trioxide, As 2 o 3 ) in the treatment of osteosarcoma. The invention belongs to the field of medical technology. Background technique [0002] Osteosarcoma is a primary malignant bone tissue tumor that occurs frequently in children and adolescents. It has strong invasive ability, rapid disease progression, and extremely high fatality rate. At present, the clinical treatment methods for patients with osteosarcoma mainly include surgery, chemotherapy, radiotherapy, interventional therapy and other comprehensive treatment methods, but surgery will cause the risk of amputation, patients' resistance to radiotherapy and chemotherapy drugs and the prognosis of osteosarcoma The extremely poor effect is still a huge problem in the treatment of osteosarcoma and the improvement of the living standards of osteosarcoma patients. Since various treatment methods bring irreve...

Claims

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Application Information

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IPC IPC(8): A61N5/06A61K33/36A61P35/00
CPCA61N5/0613A61K33/36A61N2005/0663
Inventor 杨磊蔡本志袁野杜伟杰
Owner HARBIN MEDICAL UNIVERSITY
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