The present invention discloses a protease super-concentrated laundry detergent, which mainly comprises, by mass, 3-38% of a non-ionic surfactant, 1-22% of an anionic surfactant, 0.1-1% of protease, 0.1-2% of triethanolamine, 0.1-2% of sodium citrate, 0.1-2% of refined salt and 60-70% of deionized water, wherein the protease comprises biological protease and compound protease. According to the present invention, the protease super-concentrated laundry detergent product is neutral and has characteristics of mild nature, no stimulation, soft hand-feeling, no alkaline residue after washing, no skin allergy and other symptoms, no damage on fabrics, water solubility, easy use, easy use amount control, easy storage, and easy use; and the process operation of the preparation method is simple, and the method is easily subjected to industrial production.