This invention discloses a method for preparing an antifoaming agent for aqueous laminated film glue. The antifoaming agent comprises: carrier (mainly mineral oil) 65-90 wt.%, main antifoaming agent (white carbon black, fatty acid metal soap, amide and MQ resin) 1-15 wt.%, auxiliary antifoaming agent (modified polyether, R1O(EO)x (PO)yR2) 2-20 wt.%, and emulsifier (nonionic surfactant with HLB value of 8-10) 3-15 wt.%. The method comprises: (1) stirring the primary antifoaming agent and the auxiliary antifoaming agent in the carrier, heating to 140-150 deg.C, and keeping for 2 h; (2) rapidly cooling to 70-80 deg.C, keeping for 40 min, cooling to 50 deg.C, adding the emulsifier, and stirring uniformly. The antifoaming agent has such advantages as high safely and high performance, and can be used in aqueous laminated film glue, paint, oil ink, coating cloth, etc.