The invention discloses a non-irritant mascara cream and a preparation method thereof. The mascara cream is composed of following components: decyl glucoside, lauryl glucoside, beeswax, candelilla wax, cetanol, silicone oil, polyvinylpyrrolidone, acrylic polymer, ferric oxide, polyacrylamide, (and) C13-C14 isoparaffin, (and) laureth-7, water, and addictive. The mascara cream adopts alkyl glucosides (decyl glucoside and lauryl glucoside) as the emulsifier, combines the vitamin E functions of preventing oxidation, nourishing, and repairing, and generates no allergy or discomfort to eyes after using the mascara cream. The mascara cream has the advantages of uniform coating of mascara cream body, good waterproof effect, long lasting make-up effect, no irritation, and safe using.