74 results about "Serum amyloid protein" patented technology

The invention provides a kit for quantitative detection on a serum amyloid protein A. The kit comprises a horse radish peroxidase marked monoclonal antibody of a serum amyloid protein A, a carrier wrapped by the monoclonal antibody of the serum amyloid protein A, a serum amyloid protein A calibration product, and a chemiluminiscence substrate. A preparation method of the kit comprises the following steps: marking the monoclonal antibody of the serum amyloid protein A by using horse radish peroxidase; wrapping the carrier by the monoclonal antibody of the serum amyloid protein A; preparing theserum amyloid protein A calibration product by using a pure serum amyloid protein A product; subpackaging the serum amyloid protein A calibration product, the horse radish peroxidase marked monoclonalantibody of the serum amyloid protein A, and the chemiluminiscence substrate under the action of HRP (Horse Radish Peroxidase); preparing a finished product through assembling. The kit provided by the invention is high in sensitivity, good in specificity, high in quantitative detection result accuracy, low in use cost and relatively easy to popularize and apply.

The invention discloses a serum amyloid protein A detection kit which comprises a reagent 1 and a reagent 2, and the reagent 2 comprises latex particles coated with a mouse anti-human SAA monoclonal antibody, the mouse anti-human SAA monoclonal antibody, sodium borohydride, bovine serum albumin and bovine serum albumin. According to the kit, on the basis of a chemical coupling method, aldehyde microspheres are adopted, so that the binding force of an antibody and latex is improved, and the antibody coating efficiency is further improved.

The invention relates to a serum amyloid protein A (SAA) measuring kit, and a measuring method thereof, and belongs to the technical field of medicine biological detection. The kit comprises a reagent (1), a reagent (2), a calibration material, and a quality control material. The reagent (1) comprises Tris-HCl, sodium azide, and PEG-6000. The reagent (2) comprises Tris-HCl, emulsion particles with an embedded anti-human serum amyloid protein A antibody, sodium azide, octyl phenol polyoxyethylene, and methyl paraben. The calibration material comprises a serum amyloid protein A antigen, and sodium azide. The quality control material comprises a serum amyloid protein A antigen, and sodium azide. The provided serum amyloid protein A (SAA) measuring kit and measuring method thereof have the advantages that compared with the latex immunological turbidimetry technology, the analysis sensitivity is high, the results are accurate, the operation is simple, and the cost is proper, and thus the method is convenient for popularization.

The invention discloses an enzyme-linked immunoabsorbent kit for detecting serum amyloid protein A as well as a preparation method and an application of the kit and belongs to the field of analytical test. The kit comprises an ELISA plate coated by a monoclonal antibody of a cow serum amyloid protein A, a cow serum amyloid protein A antigen standard substance, a cow serum amyloid protein A monoclonal antibody, an enzyme labeled goat anti-rat antibody, a diluent, a scrubbing solution, a developing solution and a stop buffer. The content of the serum amyloid protein A in milk is measured by a double-antibody sandwiched indirect enzyme-linked immunosorbent assay method so as to diagnose bovine subclinical mastitis. The kit disclosed by the invention is easy and convenient to operate, has the characteristics of high specificity, high sensitivity and high reproducibility and provides a novel testing tool for detection of the bovine subclinical mastitis.

The invention discloses a C-reactive protein and serum amyloid protein A combined detection test strip, a kit and a preparation method of the test strip. The C-reactive protein and serum amyloid protein A combined detection test strip comprises a bottom plate, a sample pad, a combination pad, a reaction pad and a water absorption pad, wherein the combination pad is coated with fluorescent microspheres jointly marked by a CRP antibody and a CRP broadened rabbit anti-antibody, and fluorescent microspheres jointly marked by an SAA antibody and an SAA broadened chicken anti-antibody; and a CRP detection line, an SAA detection line, a CRP widening line and an SAA widening line are formed on the reaction pad. The C-reactive protein and serum amyloid protein A combined detection test strip and the C-reactive protein and serum amyloid protein A combined detection kit containing the test strip can quantitatively detect the content of CRP and SAA in a sample at the same time, are high in sensitivity, good in specificity and high in detection speed, and further have a very wide linear range; and the linear range of CRP is 0.5-600 mg / L, the linear range of SAA is 3-800 mg / L, the operation of repeatedly diluting the sample clinically is reduced, and the clinical diagnosis efficiency is improved.