PCT/SAA combined test paper strip for rapid detection and preparation method thereof

Abstract

The invention relates to a test paper strip for (PCT / SAA) combined rapid detection of human procalcitonin / serum amyloid protein A. The test paper strip comprises a detecting card shell, a test strip, a sample pad, a gold conjugate pad coated with two colloidal gold labeled antibodies, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane is provided with a T1 detection line coated with a solid-phase matched anti-SAA monoclonal antibody, a T2 detection line coated with a solid-phase matched anti-PCT monoclonal antibody, and a control line C which is parallel to the detection line and coated with a goat anti mouse IgG monoclonal antibody. Two kinds of colloidal gold labeled antibodies are provided, which are respectively a colloidal gold labeled antibody which can be specifically combined with a to-be-detected antigen PCT and an antibody which can be specifically combined with a to-be-detected antigen SAA. The test paper strip disclosed by the invention can be used for simultaneously and rapidly detecting the PCT / SAA in a patient sample in a combined manner and has the advantages of improving the diagnosis accuracy of early inflammatory reaction of infectious diseases and being simple to operate, rapid and convenient.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a test strip for combined rapid detection of human procalcitonin (PCT) and serum amyloid A protein (SAA) and a preparation method thereof, specifically A colloidal gold immunochromatography method using the principle of the double-antibody sandwich method, combined with an immunochromatography reader, simultaneously realizes a method for on-site rapid detection of PCT / SAA two items. Background technique [0002] This product uses double-antibody sandwich method and colloidal gold immunochromatography technology to rapidly detect procalcitonin (PCT) and serum amyloid A (SAA) in human whole blood, serum or plasma samples at the same time, and can be used for inflammation and other infections Auxiliary diagnosis of such diseases. [0003] In recent years, with the development of colloidal gold immunochromatography technology and biochemical preparation technology, it has become a reality ...

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problems mentioned in the background technology, to facilitate the combined detection of procalcitonin (procalcitonin, PCT) and Serum amyloid A protein (serum amyloid A protein, SAA) content method needs to provide a PCT / SAA joint rapid detection test strip, which can solve the problem of not being able to simultaneously detect PCT / SAA in one test strip in the existing technology question

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