C-reactive protein and serum amyloid protein A combined detection test strip, kit and preparation method of test strip

Abstract

The invention discloses a C-reactive protein and serum amyloid protein A combined detection test strip, a kit and a preparation method of the test strip. The C-reactive protein and serum amyloid protein A combined detection test strip comprises a bottom plate, a sample pad, a combination pad, a reaction pad and a water absorption pad, wherein the combination pad is coated with fluorescent microspheres jointly marked by a CRP antibody and a CRP broadened rabbit anti-antibody, and fluorescent microspheres jointly marked by an SAA antibody and an SAA broadened chicken anti-antibody; and a CRP detection line, an SAA detection line, a CRP widening line and an SAA widening line are formed on the reaction pad. The C-reactive protein and serum amyloid protein A combined detection test strip and the C-reactive protein and serum amyloid protein A combined detection kit containing the test strip can quantitatively detect the content of CRP and SAA in a sample at the same time, are high in sensitivity, good in specificity and high in detection speed, and further have a very wide linear range; and the linear range of CRP is 0.5-600 mg / L, the linear range of SAA is 3-800 mg / L, the operation of repeatedly diluting the sample clinically is reduced, and the clinical diagnosis efficiency is improved.

Description

technical field [0001] The present application relates to the technical field of immunoassay, in particular to a test strip for joint detection of C-reactive protein and serum amyloid A, a kit and a preparation method for the test strip. Background technique [0002] C-reactive protein (CRP) is a part of the body's non-specific immune mechanism. It binds C-polysaccharides, and in the presence of Ca ions, it can bind to phosphorylcholine on the cell membrane, activate the classic pathway of complement, enhance leukocyte phagocytosis, and remove pathogens that invade the body. Microorganisms and damaged, necrotic, apoptotic tissue cells. [0003] Serum amyloid A (SAA) is an acute reaction protein. In a healthy state, its content in plasma is low. When the body is infected by viruses, bacteria, mycoplasma, etc., it can rise rapidly within 4-6 hours. It is about 1000 times higher, but when the stimulating factor is completely eliminated, it will decrease rapidly until it return...

AI Technical Summary

Problems solved by technology

However, the above-mentioned kit only has two detection lines and one quality control line, and the linear range of detection is relatively narrow. For high-value samples, direct detection cannot be carried out. The samples need to be diluted multiple times before loading and testing. The operation steps are cumbersome. greatly limit its application

Therefore, there is still a lack of CRP and SAA combined detection kits with the characteristics of fast, accurate quantification, and high linear range at the same time, which are used for auxiliary diagnosis

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