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Method and system for detecting cell killing efficacy and application of method and system

A detection method and cell technology, applied in biochemical equipment and methods, microbial determination/inspection, measurement devices, etc., can solve problems such as inability to collect cell images

Active Publication Date: 2021-05-18
SHANGHAI RUIYU BIOTECH
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  • Description
  • Claims
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Problems solved by technology

Secondly, although flow cytometry can specifically detect the apoptosis rate of target cells, flow cytometry is a liquid flow system and cannot collect cell images. If you need to verify the accuracy of the results, you need to use a microscope or other instruments to observe

Method used

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  • Method and system for detecting cell killing efficacy and application of method and system
  • Method and system for detecting cell killing efficacy and application of method and system
  • Method and system for detecting cell killing efficacy and application of method and system

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Embodiment Construction

[0061] The technical scheme of the present invention will be further described below in conjunction with the accompanying drawings and through specific implementation methods, but the following examples are only simple examples of the present invention, and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention is based on rights Requirements shall prevail.

[0062] In the present invention, the structure of the high-throughput cell analysis system used is as follows: figure 1 As shown, it specifically includes: a light source module, a microscopic imaging module, a sample stage module, a control module, an analysis and processing module and an automatic sample replacement module;

[0063] (1) The analysis and processing module is connected to the microscopic imaging module, and is used to analyze and process the microscopic images collected by the microscopic imaging device and count them, and obtain the co...

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Abstract

The invention provides a method and a system for detecting cell killing efficacy and application of the method and the system. The method for detecting cell killing efficacy comprises the following steps: microscopic images of a co-culture sample on a cell counting slide are acquired, the co-culture sample is a cell sample obtained by co-culturing a target cell and an effector cell, the target cell carries a first fluorescent label, the co-culture sample at least uses a second fluorescent label for dyeing labeling, the microscopic images include a bright field microscopic image, a first fluorescent microscopic image and at least one second fluorescent microscopic image, and then the bright field microscopic image, the first fluorescent microscopic image and the at least one second fluorescent microscopic image are subjected to to superposing synthetic analysis and counted, and a detection result of the cell killing efficacy is obtained. According to the detection method, the image information and the data processing result of the to-be-detected cell can be simultaneously obtained, the obtained result is more direct, few detection steps are needed, and the detection efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of cell killing activity detection, and in particular relates to a detection method, system and application of cell killing efficacy. Background technique [0002] The detection of cell killing efficacy is of great significance for the quality control of immune cell therapy products. Because immune cell therapy products have large initial individual differences, low scale of preparation process, preparations are mostly living cell products, and the mechanism of action is not very clear, etc., the consistency of such products is poor, the batch is limited, the validity period is short, and the same type Due to the poor comparability of products and other characteristics, the quality control research of immune cell therapy products is relatively complicated, and the quality control of killing efficacy is one of the difficulties. [0003] At present, the commonly used cell killing detection methods include cad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N21/64
CPCG01N33/5005G01N21/6486
Inventor 姜晶
Owner SHANGHAI RUIYU BIOTECH
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