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Serum amyloid protein A determination kit and use method thereof

A serum starch and kit technology, which is applied in biological testing, material inspection products, etc., can solve the problems of low accuracy, complicated operation, and difficult to popularize, and achieve the effects of high accuracy, simple operation, and easy popularization.

Inactive Publication Date: 2015-12-30
SHAOXING SHENG KANG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used assay kits for serum amyloid A have low accuracy and are complicated to operate, making it difficult to popularize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A serum amyloid A assay kit, comprising a first detection solution and a second detection solution, the first detection solution includes a first detection solution Tris-HCl buffer, a first detection solution sodium azide, and the The composition in terms of percentage by weight is: the first detection solution Tris-HCl buffer 98%, the first detection solution sodium azide 2%; the second detection solution includes the second detection solution Tris-HCl buffer solution, the second detection solution Sodium azide, latex particles coated with anti-human serum amyloid A antibody, the composition of each component in weight percentage is: the second detection liquid Tris-HCl buffer 96%, the second detection liquid azide 2% sodium chloride, latex particles 2% coated with anti-human serum amyloid A antibody.

[0013] A method for using a serum amyloid A assay kit, comprising the following steps: firstly preparing a first reagent tube, a second reagent tube and a third reagent...

Embodiment 2

[0016] A serum amyloid A assay kit, comprising a first detection solution and a second detection solution, the first detection solution includes a first detection solution Tris-HCl buffer, a first detection solution sodium azide, and the The composition in terms of weight percentage is: the first detection solution Tris-HCl buffer 97%, the first detection solution sodium azide 3%; the second detection solution includes the second detection solution Tris-HCl buffer solution, the second detection solution Sodium azide, latex particles coated with anti-human serum amyloid A antibody, the composition of each component in weight percentage is: the second detection liquid Tris-HCl buffer 94%, the second detection liquid azide 3% sodium chloride, 3% latex particles coated with anti-human serum amyloid A antibody.

[0017] A method for using a serum amyloid A assay kit, comprising the following steps: firstly preparing a first reagent tube, a second reagent tube and a third reagent tu...

Embodiment 3

[0020] A serum amyloid A assay kit, comprising a first detection solution and a second detection solution, the first detection solution includes a first detection solution Tris-HCl buffer, a first detection solution sodium azide, and the The composition in terms of percentage by weight is: the first detection solution Tris-HCl buffer solution 99%, the first detection solution sodium azide 1%; the second detection solution includes the second detection solution Tris-HCl buffer solution, the second detection solution Sodium azide, latex particles coated with anti-human serum amyloid A antibody, the composition of each component in weight percentage is: the second detection liquid Tris-HCl buffer 98%, the second detection liquid azide 1% sodium chloride, latex particles 1% coated with anti-human serum amyloid A antibody.

[0021] A method for using a serum amyloid A assay kit, comprising the following steps: firstly preparing a first reagent tube, a second reagent tube and a thir...

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Abstract

The invention discloses a serum amyloid protein A determination kit and a use method thereof. The serum amyloid protein A determination kit comprises first detection liquid and second detection liquid, wherein the first detection liquid includes first detection liquid Tris-HCl buffer liquid and first detection liquid sodium azide, and the second detection liquid includes second detection liquid Tris-HCl buffer liquid, second detection liquid sodium azide and latex particles coated with anti-human serum amyloid protein A antibodies. According to the serum amyloid protein A determination kit and the use method of the serum amyloid protein A determination kit, by adopting latex-enhanced immunoturbidimetry, the operation is simple, the accuracy is high, and the popularization is facilitated.

Description

technical field [0001] The present invention relates to a serum amyloid A assay kit and a using method thereof. Background technique [0002] Serumamyloid Aprotein (SAA) is an acute phase protein whose concentration in the blood can rise sharply within a few hours during inflammatory, infectious and non-infectious diseases, and can rise to 1000% of the initial concentration. fold; SAA is related to HDL and can regulate the metabolism of HDL during inflammation. A particularly important property of SAA is that its degradation products can be deposited in different organs in the form of amyloid A fibrils, which in chronic inflammatory diseases is a serious complication; elevated serum amyloid A is also seen in atherosclerosis, diabetic nephropathy, acute myocardial infarction, coronary heart disease, and chronic kidney disease. SAA has a similar role to CRP in assessing inflammation, monitoring its activity, and treating it. The SAA test is more reliable than the C-reactive ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 黄争上
Owner SHAOXING SHENG KANG BIOLOGICAL TECH
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