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Measurement kit for serum amyloid protein A

An amyloid protein and serum starch technology, applied in the field of medical biological detection, can solve the problems of undisclosed accuracy, precision and linearity, lack of calibrators and quality control materials, and unfavorable clinical promotion and use, and achieve accurate detection results. Reliable, accurate and linear results

Active Publication Date: 2019-04-09
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent publication CN108872592A discloses a latex-enhanced immune turbidimetric assay kit for serum amyloid A detection. The kit has two reagents, R1 and R2, but no calibrator and quality control product. The precision CV is 1.23%. Above, the linear deviation is above 1.81%
Chinese patent announcement CN106053862B discloses a serum amyloid A (SAA) assay kit, which also uses latex immunoturbidimetric technology, but the particle size of latex particles used is single, and its accuracy, precision and linearity are not disclosed
At present, there are few known kits for detecting serum amyloid A, and the method also has major defects, such as insufficient sensitivity, cumbersome operation, high cost, etc., which are not conducive to clinical promotion and use.

Method used

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  • Measurement kit for serum amyloid protein A
  • Measurement kit for serum amyloid protein A
  • Measurement kit for serum amyloid protein A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of Anti-human Amyloid A Antibody Latex Particles

[0040] (1) Preparation of anti-human serum amyloid A antibody solution: take the anti-human serum amyloid A antibody and dissolve it in Tris-HCl buffer solution with a pH of 6.8 to prepare an anti-human serum amyloid with a concentration of 0.5 mg / mL Protein A antibody solution;

[0041] (2) Preparation of latex microsphere suspension: According to the ratio of mass ratio of 1:3, two kinds of glycidyl methacrylate latex particles with particle diameters of 50-70nm and 100-200nm were dissolved in Tris-HCl buffer solution, the concentration was 1% (w / v), then sequentially added polyaspartic acid with a concentration of 15mg / mL and N,N-methylenebisacrylamide at a concentration of 13mg / mL, and incubated at room temperature for 45 minutes at high speed Centrifuge (12000rpm, 30min), discard the supernatant, wash the latex microspheres twice with the same volume of Tris-HCl buffer to remove excess cross...

Embodiment 2

[0043] Example 2: Preparation of Anti-human Amyloid A Antibody Latex Particles

[0044] Compared with Example 1, only the ratio of anti-human serum amyloid A antibody solution and latex microsphere suspension in step (3) is different, and the volume ratio in this example is 1:2.

Embodiment 3

[0045] Example 3: Preparation of Anti-human Amyloid A Antibody Latex Particles

[0046] Compared with Example 1, only the ratio of anti-human serum amyloid A antibody solution and latex microsphere suspension in step (3) is different, and the volume ratio in this example is 1:3.

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Abstract

The invention provides a measurement kit for serum amyloid protein A, and relates to the technical field of biomedical detection. The kit comprises a reagent R1, a reagent R2, a calibration product and a quality control product, wherein the reagent R1 comprises trihydroxy methyl aminomethane, sodium chloride, bovine serum albumin, alanine and saccharose; and the reagent R2 comprises trihydroxy methyl aminomethane, an anti-human amyloid protein A antibody latex particle, a liquid biological preservative, sodium carboxymethylcellulose, alanine, saccharose and lauryl glucoside. The kit adopts a latex immunonephelometry technology, so that a large number of samples can be detected at the same time by using a full-automatic biochemical analyzer. The kit is better in linearity, high in detectionsensitivity, correctness and precision, short in detection time, simple to operate and relatively low in economic cost.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a kit for measuring serum amyloid A. Background technique [0002] Serum amyloid A (serum amyloid A, SAA) is an acute time-limited response protein, which belongs to the heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12,000. In an acute time-limited reaction, stimulated by IL-1, IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, which can increase to 100-1000 times the initial concentration, but the half-life is extremely short. Short, only about 50 minutes. A particularly important property of serum amyloid A is that its degradation products can be deposited in different organs in the form of amyloid A fibrils, which is a serious complication in chronic inflammatory diseases. Serum amyloid A Elevation is also seen in amyloidosis, transplant rejection, coronary heart disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/544G01N33/531
CPCG01N33/531G01N33/54313G01N33/544G01N33/68G01N2333/4737
Inventor 林耀文陈青松
Owner 浙江夸克生物科技有限公司
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