30results about How to "No obvious interference" patented technology

The invention relates to a reactive rhodamine fluorescent probe for detecting mercury ions, and a preparation method thereof. A structural formula of the reactive rhodamine fluorescent probe RBHA is shown in the description. The preparation method comprises the steps of adding rhodamine B and hydrazine hydrate into a solvent, reacting for 1-2 h under refluxing at a temperature of 80-90 DEG C; carrying out rotary evaporation; adding water and adjusting pH; filtering; and purifying to obtain rhodamine B hydrazide; dissolving the rhodamine B hydrazide and glyoxylic acid in a solvent; reacting overnight with stirring at a room temperature; filtering; and purifying to obtain the reactive rhodamine fluorescent probe. The reactive rhodamine fluorescent probe has high selectivity for the mercury ions, has no obvious interference with other common ions, can work in an environment with a wide pH scope ranging from 5 to 10, and has application prospects in bioluminescent imaging and analysis of environmental water samples.

The invention provides a measurement kit for serum amyloid protein A, and relates to the technical field of biomedical detection. The kit comprises a reagent R1, a reagent R2, a calibration product and a quality control product, wherein the reagent R1 comprises trihydroxy methyl aminomethane, sodium chloride, bovine serum albumin, alanine and saccharose; and the reagent R2 comprises trihydroxy methyl aminomethane, an anti-human amyloid protein A antibody latex particle, a liquid biological preservative, sodium carboxymethylcellulose, alanine, saccharose and lauryl glucoside. The kit adopts a latex immunonephelometry technology, so that a large number of samples can be detected at the same time by using a full-automatic biochemical analyzer. The kit is better in linearity, high in detectionsensitivity, correctness and precision, short in detection time, simple to operate and relatively low in economic cost.

The invention discloses a myoglobin monoclonal abzyme marking compound. The myoglobin monoclonal abzyme marking compound is composed of a myoglobin monoclonal abzyme marker and colloidal gold, wherein the myoglobin monoclonal abzyme marker marks the colloidal gold. The invention further provides a preparation method of the myoglobin monoclonal abzyme marking compound and a myoglobin detection test kit containing the myoglobin monoclonal abzyme marking compound. The use of the myoglobin monoclonal abzyme marking compound can significantly improve the sensitivity of myoglobin detection and ensure the specificity of a reagent.

The invention discloses a water-soluble Rhodamine-based ion liquid probe for selectively distinguishing and identifying bimetallic ions and a preparation method thereof, which are applied into pure water phase to singly and selectively distinguish and identify copper ions and aluminum ions. The water-soluble Rhodamine-based ion liquid probe is prepared by dehydrating and condensing salicylaldehyde imidazole chlorate ion liquid and Rhodamine B hydrazide, and has the advantages that the cost of raw materials is low, the obtaining is easy, the preparation is simple and convenient, the water solubility is good, and the like; the trace copper and alumnum can be detected at high efficiency in pure water environment; by utilizing the obvious difference of response times of copper ions and aluminum ions, the selective distinguishing and identifying under the copper and aluminum co-existence environment is realized.

The invention relates to a method for detecting novel aminoglycoside antibiotic etimicin sulfate related substances, which comprises the following steps of: precisely weighing a proper amount of each of etimicin sulfate related substance comparison products, and adding water to the etimicin sulfate related substance comparison products respectively so as to obtain etimicin sulfate related substance comparison product stock solutions with a certain concentration; and precisely absorbing a proper amount of each of aminoglycoside related substance comparison product stock solutions, transferring the aminoglycoside related substance comparison product stock solutions to the same volumetric flask, and adding water for diluting until the required scale is reached so as to obtain aminoglycoside impurity mixed comparison product solutions, wherein flowing phases comprise a phase A and a phase B, the phase A is water-ammonia water-glacial acetic acid (96 : 3.6 : 0.4), and the phase B is methanol. A gradient elution method is employed, a measuring process for the novel aminoglycoside antibiotic etimicin sulfate related substances is carried out by using HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector), and the content of the novel aminoglycoside antibiotic etimicin sulfate related substances is calculated by an area normalization method.

The invention discloses a human insulin monoclonal antibody crosslinking magnetic particle as well as a preparation method thereof and a human insulin detection kit comprising the human insulin monoclonal antibody crosslinking magnetic particle. Before the human insulin monoclonal antibody is cross-linked with the magnetic particles, the human insulin monoclonal antibody is preprocessed by utilizing a nonionic surface active agent. The human insulin detection kit has the advantages of high detection sensitivity, strong specificity, small medicine interference, good result stability and the like; the human insulin monoclonal antibody crosslinking magnetic particle can be widely suitable for clinical auxiliary diagnosis and screening of diabetes.

The invention relates to a fluorescent probe and a preparation and application thereof. The structure of the fluorescent probe is as in formula I. The preparation method of the fluorescent probe includes the steps of dissolving 5, 7-dialdehyde-based 8 hydroxyquinoline and 2-aminothiophenol in a solvent for reflux reaction, and then conducting purification to obtain the fluorescent probe. The fluorescent probe has the advantages of being simple in synthesis, high in reaction yield and highly selective for cadmium ions, having no obvious interference to other common ions, being capable of working under an aqueous solution environment of physiological pH 7.4, and having an application prospect in bio-fluorescence imaging.

The invention relates to an lncRNA MEG3 dual detecting electrochemical gene sensor, a preparation method and an application thereof. On the basis of an enzyme assisted target circulation signal amplification and DNA chain hybridizing (HCR) signal enhancement strategy, the ultra-sensitive quantitative analysis for double component is realized through a double signal probe, under the condition of adopting a tungsten disulfide / dendritic gold nanocomposite for modifying an electrode and adopting specific complementary base pairing. The linear scope of double component detected by the electrochemical gene sensor provided by the invention is 1fM-100pM; the detection limitation is low, respectively 0.25fM and 0.3fM; due to high sensitivity, repeatability, specificity, stability and low cost, thelncRNA MEG3 dual detecting electrochemical gene sensor has higher application value in the fields of biochemical research, clinical analysis, and the like.

The invention discloses a C-peptide monoclonal antibody cross-linked with magnetic particles, a preparation method thereof and a C-peptide test kit including the same. The C-peptide monoclonal antibody is pretreated with a non-ionic surfactant after cross-linked with the magnetic particles. The invention further discloses a C-peptide enzyme conjugate diluent with trehalose for improving the stability of the enzyme conjugate. The C-peptide test kit has the advantages of high sensitivity, good specificity, low drug interfere and good stability in results, thereby being applicable to clinical diagnosis and screening of diabetes widely.

The invention discloses troponin I monoclonal antibody magnetic particles and a preparation method thereof, and a troponin detection kit comprising the troponin I monoclonal antibody magnetic particles. According to a multi-stage amplification magnetic particle crosslinking method, biotin derivative pre-crosslinked magnetic particles are adopted and then are coated with streptavidin, and the particles are closed and react with troponin I monoclonal antibody-biotin, so that the troponin I monoclonal antibody magnetic particles are prepared. The troponin I detection kit adopts a streptavidin biotin multi-stage amplification system and has the advantages of high detection sensitivity, high specificity, small medicine interference, high result stability and the like, so that the troponin I detection kit can be suitable for cardiovascular disease clinical auxiliary diagnosis on a large scale.

The invention discloses a method for measuring methanesulfonate in methanesulfonic acid by a derivatization HPLC-UV method. The method uses a dithiocarbamates derivatization reagent for derivatization of methanesulfonate to generate product having strong absorption capacity in an ultraviolet light zone; a derivative reaction liquid is taken as a sample introduction sample, based on an anti-phase high-performance liquid chromatography principle, the derivatization products of the methanesulfonate are separated and determined, so that qualitative or quantitative determination can be carried out on the methanesulfonate. According to the invention, the methanesulfonate and the dithiocarbamates derivatization reagent are subjected to a reaction, the generate product has UV absorption characteristic, and the invention establishes the simple and general method for measuring methanesulfonate in methanesulfonic acid by the derivatization HPLC-UV method. The method has good specificity and good sensitivity through results by methodology validation.

The invention discloses a method for determining phenylhydrazine and phenylhydrazine derivatives in medicine or synthetic intermediate by a derivatization HPLC-DAD method. The method is characterizedin that a benzaldehyde type derivatization reagent, and the phenylhydrazine and the phenylhydrazine derivative react to generate a product has strong absorption performance in a near visible light-visible light region; derivatization reaction liquid is used as a sample; the reaction product of the phenylhydrazine and the phenylhydrazine derivative is determined in the near visible light-visible light region on the basis of reversed-phase high performance liquid chromatography principle, so that the qualitative or quantitative detection of the phenylhydrazine and the phenylhydrazine derivativeis realized; the absorption wavelength of the derivatization product has obvious redshift, so that the substrate interference of the medicine or synthetic intermediate can be effectively avoided. Inaddition, the methodology validation result shows that the method has high specificity and sensitivity.

Provided in the invention is a method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry. The invention relates to amethod for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic. Therefore, a problem that pre treatment of the sample becomes difficult in the existing pesticide residuescreening and measuring because the ground substances of the onion, ginger and garlic are complicated and contain lots of interfering substances is solved. A DPX spearhead type dispersed solid phasemicro-extraction column is established and the residual amounts of 54 kinds of pesticides in green onion, ginger and garlic are measured by combining GC-MS / MS. The DPX method has a good purification effect and a few of operation steps; and no interference is caused on the detection result by the ground substances. The method having advantages of many test items, simplenness and rapidness, high accuracy and flux is suitable for screening and detecting the pesticides in the onion, ginger and garlic. The method is applied to the field of pesticide detection.

The invention discloses a method for removing impurity compounds in lidocaine and an obtained product, and the method comprises the following steps: using or not using inert gas protection, using hydrogen chloride gas to adjust the pH value of a reaction liquid system of lidocaine, hydrochloric acid and acetone to 2.5-3.8, and then separating and purifying. Wherein the lidocaine comprises a compound as shown in a formula II in the specification. According to the method, lidocaine can form hydrochloride and / or hydrate to improve water solubility and bioavailability, a small amount of newly found impurity compounds in lidocaine can be effectively removed, impurity limit control is carried out on lidocaine, and then it is guaranteed that the quality of a raw material medicine product is controllable; moreover, the process conditions are mild, the obtained product is high in yield and purity, low in impurity content, small in toxic and side effects and higher in safety, and the safety, effectiveness and stability of lidocaine hydrochloride (and / or hydrate thereof) and a preparation thereof can be remarkably improved.

The invention provides a preparation method of a signal turnover type photoelectrochemical biosensor for detecting cancer markers. The preparation method comprises the following steps: preparing an indium tin oxide / titanium dioxide electrode, preparing an indium tin oxide / titanium dioxide / single-chain auxiliary probe electrode, preparing a conjugate Ab1-Fe3O4 (at) Au-DNA, preparing a conjugate Ab2-AuNPs-DNA, constructing an intermediate probe and preparing the sensor. The invention also provides the biosensor prepared by the method and application thereof. The biosensor for detecting the cancer marker is based on the organic combination of a direct contact type photocurrent direction overturning strategy, a targeted induction three-dimensional dual-support DNA walker circulation signal amplification technology and a metal organic framework material adsorption characteristic, and photocurrent signal overturning design is carried out, so that the biosensor can be used for detecting the cancer marker. The biosensor can realize environment-friendly detection of cancer markers, and has the advantages of high sensitivity, high selectivity, good reproducibility, high stability and the like.

The invention discloses troponin I monoclonal antibody magnetic particles and a preparation method thereof, and a troponin detection kit comprising the troponin I monoclonal antibody magnetic particles. According to a multi-stage amplification magnetic particle crosslinking method, biotin derivative pre-crosslinked magnetic particles are adopted and then are coated with streptavidin, and the particles are closed and react with troponin I monoclonal antibody-biotin, so that the troponin I monoclonal antibody magnetic particles are prepared. The troponin I detection kit adopts a streptavidin biotin multi-stage amplification system and has the advantages of high detection sensitivity, high specificity, small medicine interference, high result stability and the like, so that the troponin I detection kit can be suitable for cardiovascular disease clinical auxiliary diagnosis on a large scale.

The invention belongs to the technical field of drug analysis and detection, and in particular relates to a method for measuring mesylate by a derivatization HPLC-UV method. Use 2-naphthylthiol derivatization reagent to derivatize mesylate to generate a derivatized product that absorbs at 252-253nm in the ultraviolet region, and use HPLC reverse-phase partition chromatography to measure methanesulfonic acid at 252-253nm in the ultraviolet region Derivatized products of esters, so as to realize the qualitative and quantitative detection of mesylate in raw materials containing methanesulfonic acid. When adopting the assay method of the present invention to measure, imatinib mesylate and other impurities in imatinib mesylate will not interfere with the analysis, and the specificity of the method is good; the detection limit of the method is 0.03 μg / ml, the limit of quantification is 0.10 μg / ml, and the linear relationship is good; the average recovery rate is between 87.5‑102.1%, without obvious matrix interference; and the derivatized product has good stability within 6 hours.

The invention belongs to the technical fields of analysis and detection of drugs and particularly relates to a method for measuring methanesulfonate by virtue of a derivatization HPLC-UV method. According to the method, methanesulfonate is derivatized by virtue of a 2-thionaphthol derivatization agent to generate a derivated product with absorptivity in an ultraviolet region of 252nm-253nm, and the derivated product of methanesulfonate is measured in the ultraviolet region of 252nm-253nm by virtue of an HPLC reversed phase partition chromatography, thereby realizing the qualitative and quantitative determination of methanesulfonate in a raw material drug containing methanesulfonic acid. In the measurement process of the method, the analysis is not interfered by imatinib mesylate and otherimpurities in imatinib mesylate, so that the specificity of the method is good; the detection limit of the method is 0.03microg / ml, the quantitation limit is 0.10microg / ml, and the linear relation isgood; the average recovery rate is 87.5%-102.1%, and the obvious matrix interference avoided; and the derivated product has good stability within 6 hours.

The invention relates to an lncRNA MEG3 dual detecting electrochemical gene sensor, a preparation method and an application thereof. On the basis of an enzyme assisted target circulation signal amplification and DNA chain hybridizing (HCR) signal enhancement strategy, the ultra-sensitive quantitative analysis for double component is realized through a double signal probe, under the condition of adopting a tungsten disulfide / dendritic gold nanocomposite for modifying an electrode and adopting specific complementary base pairing. The linear scope of double component detected by the electrochemical gene sensor provided by the invention is 1fM-100pM; the detection limitation is low, respectively 0.25fM and 0.3fM; due to high sensitivity, repeatability, specificity, stability and low cost, thelncRNA MEG3 dual detecting electrochemical gene sensor has higher application value in the fields of biochemical research, clinical analysis, and the like.

The invention discloses a human insulin monoclonal antibody crosslinking magnetic particle as well as a preparation method thereof and a human insulin detection kit comprising the human insulin monoclonal antibody crosslinking magnetic particle. Before the human insulin monoclonal antibody is cross-linked with the magnetic particles, the human insulin monoclonal antibody is preprocessed by utilizing a nonionic surface active agent. The human insulin detection kit has the advantages of high detection sensitivity, strong specificity, small medicine interference, good result stability and the like; the human insulin monoclonal antibody crosslinking magnetic particle can be widely suitable for clinical auxiliary diagnosis and screening of diabetes.

The invention discloses a primary air automatic leveling algorithm for a power station boiler, which comprises the following steps: (1) after receiving primary air speed measured by a pipeline grinding sensor, a leveler analyzes whether the primary air speed needs to be adjusted or not; (2) when adjustment is needed, a pipeline with the maximum primary air speed and a pipeline with the minimum primary air speed are found and marked as Pmax and Pmin; (3) when a Pmin throttling valve is not fully opened, a pre-adjusting pipeline is Pmin and is adjusted in the opening direction; and when the Pmin throttle valve is fully opened, the pre-adjusting pipeline is Pmax and is adjusted towards the closing direction; (4) whether a risk exists after the adjusted pipeline is adjusted is judged, and the process enters the next period if the risk exists; and (5) the adjusted pipeline throttle valve acts, and then the next period is started. The primary air automatic leveling algorithm has the following beneficial effects: 1, at least one pipeline throttle valve is ensured to be fully opened when one mill is used for leveling, and the resistance to primary air conveying is minimum; 2, only one pipeline is operated in each period, so that the safe operation of a unit is not obviously interfered; and 3, the primary air of the mill is leveled intelligently, and manpower is saved.

A kind of DPX-GC-MS detection method of 2-clodiazepam and 4-clodiazepam in urine, it relates to the field of forensic science toxicology analysis, the purpose of the present invention is to solve the problem of 2-clodiazepam in urine Pam and 4-chlordiazepam need to use different organic solvents, manual operation, time-consuming, laborious and low efficiency problems, the present invention adopts the pipette type dispersed solid-phase microextraction column that is filled with HLB filler to extract and purify samples, and establishes A GC-MS high-throughput and rapid detection method for 2-clodiazepam and 4-clodiazepam residues in urine. The method can screen and analyze 2-clodiazepam and 4-clodiazepam in urine, meeting the testing needs of actual cases. The invention is applied to the field of forensic science urine detection.

The invention discloses a water-soluble Rhodamine-based ion liquid probe for selectively distinguishing and identifying bimetallic ions and a preparation method thereof, which are applied into pure water phase to singly and selectively distinguish and identify copper ions and aluminum ions. The water-soluble Rhodamine-based ion liquid probe is prepared by dehydrating and condensing salicylaldehyde imidazole chlorate ion liquid and Rhodamine B hydrazide, and has the advantages that the cost of raw materials is low, the obtaining is easy, the preparation is simple and convenient, the water solubility is good, and the like; the trace copper and alumnum can be detected at high efficiency in pure water environment; by utilizing the obvious difference of response times of copper ions and aluminum ions, the selective distinguishing and identifying under the copper and aluminum co-existence environment is realized.

The invention discloses a C-peptide monoclonal antibody cross-linked with magnetic particles, a preparation method thereof and a C-peptide test kit including the same. The C-peptide monoclonal antibody is pretreated with a non-ionic surfactant after cross-linked with the magnetic particles. The invention further discloses a C-peptide enzyme conjugate diluent with trehalose for improving the stability of the enzyme conjugate. The C-peptide test kit has the advantages of high sensitivity, good specificity, low drug interfere and good stability in results, thereby being applicable to clinical diagnosis and screening of diabetes widely.

The invention discloses a method for measuring methanesulfonate in methanesulfonic acid by a derivatization HPLC-UV method. The method uses a dithiocarbamates derivatization reagent for derivatization of methanesulfonate to generate product having strong absorption capacity in an ultraviolet light zone; a derivative reaction liquid is taken as a sample introduction sample, based on an anti-phase high-performance liquid chromatography principle, the derivatization products of the methanesulfonate are separated and determined, so that qualitative or quantitative determination can be carried out on the methanesulfonate. According to the invention, the methanesulfonate and the dithiocarbamates derivatization reagent are subjected to a reaction, the generate product has UV absorption characteristic, and the invention establishes the simple and general method for measuring methanesulfonate in methanesulfonic acid by the derivatization HPLC-UV method. The method has good specificity and good sensitivity through results by methodology validation.

The invention relates to the technical field of electrochemical detection, and specifically discloses a chemically modified electrode for detecting cephalexin and an electrochemical determination method for cephalexin. The chemically modified electrode is prepared by a method comprising the following steps: drop a colloidal gold solution on the surface of the cleaned gold electrode, dry it and immerse it in a hydrochloric acid solution containing cysteine, and automatically Assemble, then take it out and rinse with water. The electrochemical determination method comprises the steps of: adding a copper sulfate solution to the sample solution to be tested for hydrolysis to obtain a hydrolyzate, using the above-mentioned chemically modified electrode as a working electrode, and measuring the remaining concentration in the hydrolyzate by square wave voltammetry The concentration of copper ions, thereby indirectly measuring the content of cephalexin in the sample. Using the chemically modified electrode prepared by the invention to measure cephalexin has the advantages of high sensitivity, quickness, convenience, low cost and the like.

The invention relates to a fluorescent probe and its preparation and application. The structure of the probe is as formula I. Preparation: Dissolve 5,7-dialdehyde-8-hydroxyquinoline and 2-aminothiophenol in a solvent, reflux reaction, and purify to obtain that product. The fluorescent probe of the present invention is simple to synthesize, has high reaction yield, is highly selective to cadmium ions, and has no obvious interference with other common ions; it can work under the environmental conditions of an aqueous solution with a physiological pH of 7.4, and has applications in bioluminescence imaging prospect.

The invention discloses lidocaine impurities, application of the lidocaine impurities in a detection method and the detection method. The lidocaine impurity compound disclosed by the invention is a compound as shown in a formula I which is described in the specification. The lidocaine impurity compound provides a basis for quality and safety evaluation of lidocaine or intermediates, salts, crystal forms and solvates of lidocaine, and is of great significance in improving the quality of lidocaine or intermediates, salts, crystal forms and solvates of lidocaine.