Colloidal coomassie stain

Abstract

Colloidal formulation for staining proteins and methods of their use are provided.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Application Serial No. 61 / 777,801, filed March 12, 2013, which is incorporated herein by reference. Background of the invention [0003] Electrophoresis gels can be stained to a defined endpoint with Coomassie Brilliant Blue G-250, since the dye can be prepared in colloidal form, which does not enter the gel to be stained, but is absorbed by the protein bands in the gel, Allow sufficient time to diffuse throughout the strip to saturate the strip. Because the colloidal form does not enter the gel itself, the background is very low, thereby reducing the need for a destaining step that can result in a decrease in the intensity of the stained protein bands. [0004] Electrophoresis gels are usually stained with two-part stains. The 2-part stain needs to be prepared fresh before each use to have the desired properties (eg, low background). 2-Part stains quickly form...

AI Technical Summary

Problems solved by technology

Simply substituting ethanol for methanol would render the formulation unacceptable

Images