Rapid protein staining solution

Abstract

The invention discloses a rapid protein staining solution. The rapid protein staining solution is used for protein staining in SDS-PAGE. The rapid protein staining solution comprises, by mass volume,0.05%-0.1% of coomassie brilliant blue G250, 5%-15% of sulfate, 5%-20% of absolute ethyl alcohol, 1%-5% of polyethylene glycol and 1%-10% of an organic acid. The rapid protein staining solution is optimized on the basis of a traditional staining solution, a clearer protein strip is obtained, background staining is basically avoided, a decolorizing solution does not need to be used for decolorizingafter staining is finished, only tap water is used for washing, the detection time is greatly shortened, the efficiency is improved, no toxic or harmful reagent is used in the staining process, and harm to a human body is avoided.

Description

technical field [0001] The invention relates to the field of SDS-PAGE protein staining, in particular to a rapid protein staining solution. Background technique [0002] Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has become the most commonly used protein analysis and detection method in molecular biology experiments. After separating proteins by electrophoresis, the gel is stained to detect the protein in the gel. of protein. Among them, protein staining is one of the key steps that affect the sensitivity of protein detection. Commonly used protein staining methods include the following: [0003] (1) Silver staining, which is one of the most sensitive protein staining methods, can detect proteins at the nanogram level, however, this method uses a variety of reagents with strong odor and irritation, such as formaldehyde, glutaraldehyde, methanol and acetic acid, and the experimental repetition rate is low, in addition, stained proteins are sometime...

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Problems solved by technology

[0003] (1) Silver staining, which is one of the most sensitive protein staining methods, can detect proteins at the nanogram level, however, this method uses a variety of reagents with strong odor and irritation, such as formaldehyde, glutaraldehyde, methanol and acetic acid, and the experimental repetition rate is low, in addition, stained proteins are sometimes incompatible with downstream mass spectrometry (MS) proteomics analysis

[0004] (2) Fluorescent staining method, which often uses SYPRO Ruby, Deep Purple or Flamingo dyes for fluorescent staining, can also detect proteins in the nanogram range, but also requires the use of strong odor and irritating reagents, and the price of reagents Expensive, plus, fluorescent staining requires relatively long processing times and requires special equipment such as fluorescent imaging scanners and advanced software

[0007] However, none of the above methods can get rid of the irritating and volatile solvents such as acetic acid or methanol, and these reagents will cause serious harm to the human respiratory system during preparation and use.

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