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Method for preparing quality control product for five-class blood cell analyze

A blood cell analyzer and five-classification technology, applied in the field of quality control material preparation for five-class blood cell analyzers, can solve the problems of differences in measured values, difficulty in evaluating and interpreting results, etc., achieving low production costs, filling domestic gaps, and ensuring The effect of consistency

Inactive Publication Date: 2019-06-28
江苏中济万泰生物医药有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defect in the prior art that the measured values ​​of the analytical instruments of the manufacturers of the blood cell analyzers are different, which brings certain difficulties to the evaluation and interpretation results, and to provide a five-classification blood cell analysis Method for preparing quality control products for instruments

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  • Method for preparing quality control product for five-class blood cell analyze
  • Method for preparing quality control product for five-class blood cell analyze

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Embodiment

[0027] 1. A method for preparing a quality control product for a five-differentiation hematology analyzer

[0028] The first step, the preparation of fixative:

[0029] Fixative solution A: 0.3% formaldehyde, 0.15% glutaraldehyde, 1% methanol;

[0030] Fixative solution B: formaldehyde 0.7%, glutaraldehyde 0.2%, ethanol 2%;

[0031] Fixative solution C: formaldehyde 1%, glutaraldehyde 0.5%, methanol 0.5%, ethanol 2%.

[0032] The second step, the preparation of blood cell preservation solution:

[0033] Trisodium citrate 14.7g, glucose 27.0g, adenine 0.4g, sodium chloride 8.2g, edetate disodium 0.3g, inosine 2.7g, potassium dihydrogen phosphate 0.122g, disodium hydrogen phosphate 0.716 g, 13.8g of sucrose, 1.8g of polyethylene glycol 4000, 0.7g of chloramphenicol, 1.0g of neomycin sulfate, add purified water to 2000ml.

[0034] The third step is to weigh the "O" type fresh blood that has passed the inspection by a professional organization, perform a complete blood cell co...

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Abstract

The invention discloses a method for preparing a quality control product for a five-class blood cell analyzer. The method comprises: step one, preparing fixing liquid; step two, preparing blood cell preservation liquid; step three, carrying out preliminary fixation on whole blood for several hours, carrying out centrifugal separation, fixing red blood cells and blood platelets again for several hours and then carrying out centrifugal separation, washing a PBS buffer solution with the pH of 7.2 for various blood cells and removing the fixing liquid for standby; step four, allocating cell concentration values form first to third levels or from first to fifth levels for each standard; step five, adding the blood cell preservation liquid into the fixed blood cells, carrying out mixing, carrying out sampling detection, and carrying fine adjusting based on the detection result; and step six, after successful detection, carrying out subpackaging, labeling, and sampling and quality checking after packaging. Therefore, the consistency with the clinical test specimen medium and the cell parameter is ensured; the precision of the whole blood cell measurement work of the quality control laboratory in the clinical laboratory is met; and the accuracy change is detected. The quality control product can reach the quality of the similar foreign products.

Description

technical field [0001] The invention relates to a method for preparing a quality control product for a five-classification blood cell analyzer, and the invention belongs to the field of biotechnology. Background technique [0002] As a routine blood test item, blood cell analysis plays an indispensable and important role in clinical diagnosis and treatment. Especially in the diagnosis of infectious diseases and blood diseases. Therefore, it is very important to ensure the accuracy of blood routine test results in the daily inspection work of the laboratory department of the hospital. [0003] The quality control product for five-differentiation blood cell analyzer is a substance used to check the accuracy of the analysis process (or instrument) in the routine work of blood cell analysis. The substance is similar in properties to the specimen being analyzed and is analyzed along with the patient sample. [0004] The necessity of quality control products for five-classifica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 陈亚平吴明磊华强
Owner 江苏中济万泰生物医药有限公司
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