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660results about "Microscopic object acquisition" patented technology

Method and system for processing regions of interest for objects comprising biological material

A method and apparatus are disclosed for processing regions of interest for objects comprising biological material. A region of interest can be denoted for a physical object and information indicating the region of interest can be stored in a computer-readable medium for later retrieval. Subsequently, when the object is retrieved, the information indicating the region of interest can be used to generate information specifying a physical location within the region of interest. An operation can then be performed on the physical location within the region of interest. Reference pints within the object can assist in regeneration of the region of interest, and the reference points can be arranged in such a fashion that processing can take rotation of the object into account. The invention includes various features advantageous for constructing tissue microarrays.
Owner:GOVERNMENT OF THE UNITED STATES OF AMERICAS AS REPRESENTED BY THE SEC OF THE DEPT OF HEALTH & HUMAN SERVICES THE +1

Rapid Microscope Scanner for Volume Image Acquisition

Apparatus for and method of rapid three dimensional scanning and digitizing of an entire microscope sample, or a substantially large portion of a microscope sample, using a tilted sensor synchronized with a positioning stage. The system also provides a method for interpolating tilted image layers into a orthogonal tree dimensional array or into its two dimensional projection as well as a method for composing the volume strips obtained from successive scans of the sample into a single continuous digital image or volume.
Owner:LEICA BIOSYST IMAGING

System and method for generating digital images of a microscope slide

An improved system and method for obtaining images of a microscope slide are provided. In one embodiment, a focus camera includes an optical sensor that is tilted relative to the focal plane of a scanning camera. A scan of a target region is performed, and multiple overlapping images of the target region are captured from a plurality of x-y positions. Each image contains information associated with multiple focal planes. Focus information is obtained from the images, and a desired-focus position is determined for the target region based on the focus information. The scanning camera then captures an image of the target region from the desired-focus position. This procedure may be repeated for selected regions on the microscope slide and the resulting images of the respective regions are merged to create a virtual slide.
Owner:CARL ZEISS MICROIMAGING AIS

Image quality assessment including comparison of overlapped margins

Image quality is assessed for a digital image that is a composite of tiles or other image segments, especially focus accuracy for a microscopic pathology sample. An algorithm or combination of algorithms correlated to image quality is applied to pixel data at margins where adjacent image segments overlap and thus contain the same content in separately acquired images. The margins may be edges merged to join the image segments smoothly into a composite image, and typically occur on four sides of the image segments. The two versions of the same image content at each margin are processed by the quality algorithm, producing two assessment values. A sign and difference value are compared with other image segments, including by subsets selected for the orientation of the margins on sides on the image segments. The differences are mapped to displays. Selection criteria determine segments to be re-acquired.
Owner:GENERAL ELECTRIC CO +1

High Resolution Structured Light Source

A structured light source comprising VCSEL arrays is configured in many different ways to project a structured illumination pattern into a region for 3 dimensional imaging and gesture recognition applications. One aspect of the invention describes methods to construct densely and ultra-densely packed VCSEL arrays with to produce high resolution structured illumination pattern. VCSEL arrays configured in many different regular and non-regular arrays together with techniques for producing addressable structured light source are extremely suited for generating structured illumination patterns in a programmed manner to combine steady state and time-dependent detection and imaging for better accuracy. Structured illumination patterns can be generated in customized shapes by incorporating differently shaped current confining apertures in VCSEL devices. Surface mounting capability of densely and ultra-densely packed VCSEL arrays are compatible for constructing compact on-board 3-D imaging and gesture recognition systems.
Owner:PRINCETON OPTRONICS

Automated microscope slide tissue sample mapping and image acquisition

A method comprises receiving an image of a tissue-sample set. A position in the image of each tissue sample relative to at least one other tissue sample is electronically identified. Each tissue sample is electronically identified based on the tissue sample position identification.
Owner:THE CHAMBERLAIN GRP INC +1

Whole Slide Fluorescence Scanner

A whole slide fluorescence digital pathology system is provided that uses a monochrome TDI line scan camera, which is particularly useful in fluorescence scanning where the signal is typically much weaker than in brightfield microscopy. The system uses oblique brightfield illumination for fast and accurate tissue finding and employs a unique double sweep focus scoring and objective lens height averaging technique to identify focus points and create a focus map that can be followed during subsequent scanning to provide autofocusing capability. The system also scans and analyzes image data to determine the optimal line rate for the TDI line scan camera to use during subsequent scanning of the digital slide image and it also creates a light profile to compensate for loss of illumination light due to roll off.
Owner:LEICA BIOSYST IMAGING

System for creating microscopic digital montage images

An imaging apparatus is disclosed. The imaging apparatus includes a motorized stage, a camera focussed relative to the stage, and a processor coupled to the camera. The processor contains instructions which, when executed by the processor, cause the processor to capture an image incident on the camera, convert the image into a plurality of pixels having a characteristic such as intensity, establish the characteristic for each pixel; and determine which pixels contain a target image based on the characteristic of the pixels. Another imaging apparatus includes a motorized stage, a camera having a lens directed toward the motorized stage, and a processor coupled to the camera. The processor contains instructions which, when executed by the processor, cause the processor to select at least three regions of a sample adjacent the motorized stage, develop a focus surface based on a normalized distribution of the at least three selected regions, position the camera on a region of the sample, focus the camera on the surface, and capture an image of the region. In addition, an imaging apparatus having a motorized stage, a camera focussed relative to the motorized stage, a stage position sensor adjacent the motorized stage, and a pulsed light directed toward the motorized stage and coupled to the stage position sensor such that the pulsed light illuminates in response to the stage position sensor is disclosed.
Owner:CARL ZEISS MICROSCOPY GMBH

Method and apparatus for automated image analysis of biological specimens

A method and apparatus for automated cell analysis of biological specimens automatically scans at a low magnification to acquire images which are analyzed to determine candidate cell objects of interest. The low magnification images are converted from a first color space to a second color space. The color space converted image is then low pass filtered and compared to a threshold to remove artifacts and background objects from the candidate object of interest pixels of the color converted image. The candidate object of interest pixels are morphologically processed to group candidate object of interest pixels together into groups which are compared to blob parameters to identify candidate objects of interest which correspond to cells or other structures relevant to medical diagnosis of the biological specimen. The location coordinates of the objects of interest are stored and additional images of the candidate cell objects are acquired at high magnification. The high magnification images are analyzed in the same manner as the low magnification images to confirm the candidate objects of interest which are objects of interest. A high magnification image of each confirmed object of interest is stored for later review and evaluation by a pathologist.
Owner:CARL ZEISS MICROSCOPY GMBH

Apparatus and methods for verifying the location of areas of interest within a sample in an imaging system

An apparatus and method for verifying the location of an area of interest within a sample are disclosed. An imaging system includes an optical system and a stage movable relative to the optical system. A computer server is in communication with the imaging system and with a review station. The imaging system is capable of spatially locating a datum mark on the sample and determining a spatial offset value of the mark relative to a nominal position thereof. The coordinate systems of a respective one of the imaging system and the review station can be standardized. The method includes locating a datum mark on the sample, and identifying an area of interest within the sample. The method further includes determining the location of the area of interest relative to the mark. The method further includes locating again the datum, and checking that a dimensional error in locating the datum mark is less than a tolerance value to verify location of the area of interest.
Owner:CYTYC CORP

Four-dimensional imaging of periodically moving objects via post-acquisition synchronization of nongated slice-sequences

InactiveUS20050259864A1Fast executionMicroscopic object acquisitionLeast squares minimizationOrganism
When the studied motion is periodic, such as for a beating heart, it is possible to acquire successive sets of two dimensional plus time data slice-sequences at increasing depths over at least one time period which are later rearranged to recover a three dimensional time sequence. Since gating signals are either unavailable or cumbersome to acquire in microscopic organisms, the invention is a method for reconstructing volumes based solely on the information contained in the image sequences. The central part of the algorithm is a least-squares minimization of an objective criterion that depends on the similarity between the data from neighboring depths. Owing to a wavelet-based multiresolution approach, the method is robust to common confocal microscopy artifacts. The method is validated on both simulated data and in-vivo measurements
Owner:CALIFORNIA INST OF TECH

Apparatus and Method for Rapid Microscope Image Focusing

A method of capturing a focused image of a continuously moving slide / objective arrangement is provided. A frame grabber device is triggered to capture an image of the slide through an objective at a first focus level as the slide continuously moves laterally relative to the objective. Alternatingly with triggering the frame grabber device, the objective is triggered to move to a second focus level after capture of the image of the slide. The objective moves in discrete steps, oscillating between minimum and maximum focus levels. The frame grabber device is triggered at a frequency as the slide continuously moves laterally relative to the objective so multiple images at different focus levels overlap, whereby a slide portion is common to each. The image having the maximum contrast value within overlapping images represents an optimum focus level for the slide portion, and thus the focused image. Associated apparatuses and methods are also provided.
Owner:TRIPATH IMAGING INC

Auto focus for a flow imaging system

A pair of optical gratings are used to modulate light from an object, and the modulated light from either optical is used to determine the velocity of the object. Each optical grating is offset from a reference focal point by the same distance, one grating being offset in a positive direction, the other in a negative direction. Signals produced in response to the modulated light can be processed to determine a direction in which a primary collection lens should be moved in order to improve a focus of the imaging system on the object. The lens is moved incrementally in the direction so determined, and the process is repeated until an optimal focus is achieved. In a preferred embodiment, the signals are weighted, so that the optical grating disposed closest to the optimal focus position contributes the most to velocity detection.
Owner:CYTEK BIOSCI

Extended electron tomography

A method for improving image resolution of a three dimensional structure of at least one molecule conformation includes: determining a three dimensional structure of at least one conformation of a molecule in a sample from a first data set obtained from a series of 2D measurements of different geometrical projections of the molecule at a low electron beam dose in an electron microscope; producing a second data set including calculated two dimensional projections of the determined three dimensional structure of the at least one conformation of the same molecule; correlating data from a third data set obtained from at least one measurement of the same molecule using a higher electron beam dose with the second data set; and using the correlated data to improve the resolution of the three dimensional structure of the at least one conformation of the molecule by increasing the first data set with the correlated data and re-determining a three dimensional structure.
Owner:OKINAWA INST OF SCI & TECH PROMOTION CORP

Fourier Ptychographic X-ray Imaging Systems, Devices, and Methods

Methods, systems, and devices of Fourier ptychographic X-ray imaging by capturing a plurality of variably-illuminated, low-resolution intensity X-ray images of a specimen and computationally reconstructing a high-resolution X-ray image of the specimen by iteratively updating overlapping regions in Fourier space with the variably-illuminated, low-resolution intensity X-ray images.
Owner:CALIFORNIA INST OF TECH

Measurement apparatus, method and computer program

A measurement apparatus for enumeration of particles or white blood cells in a sample comprises: a holder, which is arranged to receive a sample acquiring device that holds a sample, an imaging system, comprising a magnifying means and at least one digital image acquiring means, said imaging system being arranged to acquire at least one digital image of the sample, and an image analyser, which is arranged to analyse the digital image for identifying particles or white blood cells and determining the number of particles or white blood cells and which is arranged to analyse the digital image for identifying particles or white blood cells that are imaged in focus, determining types of these particles or white blood cells, the types being distinguished by physical features and determining the ratio of different types of particles or white blood cells.
Owner:HEMOCUEAB

Display Apparatus Capable of Image Scanning and Driving Method Thereof

According to an embodiment of the present invention, a display apparatus capable of image scanning and a method for driving the same are provided.According to an embodiment of the present invention, a display apparatus capable of image scanning is provided including a contact sensor arranged in each unit pixel. The contact sensor includes a pixel electrode forming a contact capacitance by contact with a contact means; a reset transistor where a drain electrode is connected to a node where the contact capacitance is formed, and each of a gate electrode and a source electrode is connected to a first scan line to which a selective signal is applied; an amplifying transistor where a gate electrode is connected to the drain electrode of the reset transistor, and a source electrode is connected to a power input terminal; and a detecting transistor where a drain electrode is connected to the drain electrode of the amplifying transistor, a gate electrode is connected to a second scan line to which a selective signal is applied, and a source electrode is connected to a readout line detecting a current corresponding to the contact capacitance.
Owner:CRUCIALTEC

Biological Fluid Analysis System and Method

A biological fluid analysis system and method for measuring optical characteristics of a sample of biological fluid using a commercially available portable computing device having a camera, such as a smart phone. The system includes a scope, a case that attaches to the portable computing device, and a software application that runs on the portable computing device. Some embodiments of the invention include a sample slide having a viewing chamber that can be filled with a biological fluid to be analyzed by the biological fluid analysis system. The system may be adapted to analyze cow's milk to estimate the number of somatic cells per unit volume contained in the milk using a reagent that stains the somatic cells so that they will fluoresce when excited by light with a particular wavelength, with the light source in the scope being adapted to generate light of that particular wavelength.
Owner:DAIRY QUALITY

System and methods for generating a brightfield image using fluorescent images

A method for generating a brightfield type image, which resembles a brightfield staining protocol of a biological sample, using fluorescent images is provided. The steps comprise acquiring two or more fluorescent images of a fixed area on a biological sample, mapping said fluorescent image into a brightfield color space, and generating a bright field image. Also provided is an image analysis system for generating a brightfield type image of a biological sample using fluorescent images.
Owner:LEICA MICROSYSTEMS CMS GMBH

Image processing method and system for microfluidic devices

A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.
Owner:FLUIDIGM CORP

Method and apparatus for recognition of microorganisms using holographic microscopy

Disclosed herein is a method that relates to identifying a microorganism. The method comprising, diffracting laser light through a microorganism, combining a reference beam with the diffracted light on a single axis, and recording the combined light holographically as a three-dimensional image with a single exposure on a detector array. The method further comprising, reconstructing the holographic image and matching the reconstructed image with reference images of known microorganisms. Further disclosed herein is an apparatus for identifying a microorganism. The apparatus comprising, an interferometer to record an image of an unknown microorganism using single-exposure on-line (SEOL) digital holography, a means for reconstructing the recorded image, and a means for matching the reconstructed image with images of known microorganism.
Owner:UNIV OF CONNECTICUT

Personal airborne particle monitor with quantum dots

An airborne biological particle monitoring device collects particles floating in air. The monitor includes a camera sensor, illumination source, and quantum-dot illumination source. The camera sensor captures a first image of the particles when the collected particles are illuminated by the illumination source. The camera sensor captures a second image of the particles when the collected particles are illuminated by the quantum-dot illumination source. The first and second images are analyzed to identify the collected particles.
Owner:SCANIT TECH INC

Measurement apparatus, method and computer program

A measurement apparatus for enumeration of particles or white blood cells in a sample comprises: a holder, which is arranged to receive a sample acquiring device that holds a sample, an imaging system, comprising a magnifying means and at least one digital image acquiring means, said imaging system being arranged to acquire at least one digital image of the sample, and an image analyser, which is arranged to analyse the digital image for identifying particles or white blood cells and determining the number of particles or white blood cells and which is arranged to analyse the digital image for identifying particles or white blood cells that are imaged in focus, determining types of these particles or white blood cells, the types being distinguished by physical features and determining the ratio of different types of particles or white blood cells.
Owner:HEMOCUEAB
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