Platelet-rich plasma rapid separating device and method
A platelet-rich plasma and platelet-rich technology, applied in chemical instruments and methods, centrifuges, laboratory equipment, etc., can solve the problems of limiting the clinical application of platelet-rich plasma, slow enrichment speed of platelet-rich plasma, high preparation cost, etc. Achieve the effects of increasing concentration and preparation efficiency, solving long preparation cycle and low cost
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Embodiment 1
[0064] A device for rapidly extracting high-concentration platelet-rich plasma, including a centrifuge tube, the centrifuge tube includes an inner tube body, an outer tube body and a sealing cover sleeved on the inner tube body, and the upper part of the inner tube body in sealing contact with the sealing cover is a hard It is made of high-quality polymer materials, and the rest is made of soft polymer materials. There is a contraction channel in the middle, and the inner tube body and the outer tube body are set together; the contraction channel is equipped with a buckle device, which can close the contraction channel. The outer tube body is a conical centrifugal tube made of hard polymer material; the top surface of the sealing cover body is provided with a syringe needle penetrable area, and the side lower edge of the sealing cover body and the upper edge of the outer tube body are in the inner tube body external connections.
[0065] The inner tube body is made of polyethy...
Embodiment 2
[0083] The method for one-step separation and preparation of low-concentration platelet-rich plasma using a device for rapidly extracting high-concentration platelet-rich plasma is characterized by comprising the following steps:
[0084] A. Collect 25ml of peripheral blood from volunteers for anticoagulation;
[0085] B. Add the blood sample into a 50ml centrifuge tube with a flat needle syringe;
[0086] C. Put the centrifuge tube into the centrifuge and centrifuge for 10 minutes at a centrifugal force of 1000g. The blood is divided into three layers: the upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer;
[0087] D. If the buckle is a split type buckle, lift the centrifuge tube with the cover out of the inner tube, use the buckle to close the contraction channel at the interface between plasma and red blood cells, and put the inner tube back into the outer tube; if the buckle is integrated Buckle, then ...
Embodiment 3
[0090] The method for two-step separation and preparation of high-concentration platelet-rich plasma using a device for rapidly extracting high-concentration platelet-rich plasma is characterized by comprising the following steps:
[0091] A. Collect 25ml of peripheral blood from volunteers for anticoagulation;
[0092] B. Add the blood sample into a 50ml centrifuge tube with a flat needle syringe;
[0093] C. Put the centrifuge tube into the centrifuge and centrifuge for 10 minutes at a centrifugal force of 1000g. The blood is divided into three layers. The upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer;
[0094] D. If the buckle is a split type buckle, lift the centrifuge tube with the cover out of the inner tube, use the buckle to close the contraction channel at the interface between plasma and red blood cells, and put the inner tube back into the outer tube; if the buckle is integrated Buckle, then...
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